Creating coveted bioluminescence colors for simultaneous myriad-color bioimaging DOI Creative Commons
Mitsuru Hattori, Tetsuichi Wazawa,

Mariko Orioka

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Abstract Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of bioluminescent proteins must sufficiently vary enable simultaneous detection. Here, we aimed introduce method can be used expand color variation tuning dual-acceptor bioluminescence resonance energy transfer (BRET). Using this approach, could visualize targets with up 20 through single-shot acquisition using CMOS camera. Overall, enables simple and observation numerous biological phenomena.

Language: Английский

Creating coveted bioluminescence colors for simultaneous multi-color bioimaging DOI Creative Commons
Mitsuru Hattori, Tetsuichi Wazawa,

Mariko Orioka

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(4)

Published: Jan. 22, 2025

Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of bioluminescent proteins must sufficiently vary enable simultaneous detection. Here, we aimed introduce method can be used expand color variation tuning dual-acceptor bioluminescence resonance energy transfer. Using this approach, could visualize targets with up 20 through single-shot acquisition using complementary metal-oxide semiconductor camera. Overall, enables simple and observation biological phenomena.

Language: Английский

Citations

1
The latest developments of near-infrared fluorescent probes from NIR-I to NIR-II for bioimaging DOI Open Access

Yuzuka Kuronuma,

Reiko Watanabe, Yuki Hiruta

et al.

Analytical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Near-infrared I (NIR-I: 650–950 nm) fluorescence imaging is a powerful tool for deep-tissue biological imaging, addressing the limitations of photon penetration depth in visible-light region. Over past decade, NIR has extended to near-infrared II (NIR-II: 1000–1700 region, offering high-resolution and low background by suppressing light scattering, autofluorescence tissues. fluorescent probes from NIR-I NIR-II, with diverse functionalities, are increasingly utilized across fields meet various detection needs explore physiological events real time spatial dimensions. This review discusses recent advancements small-molecule dyes probes, particularly those based on cyanine rhodamine scaffolds, highlighting examples their applications bioimaging.

Language: Английский

Citations

0
Exploring the Synergy Between Bioluminescence and Nanomaterials: Innovations in Analytical and Therapeutic Applications DOI
Polina M. Ivantcova,

Adilya R Sungatullina,

Kristina V Pidgirnaya

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: unknown, P. 114631 - 114631

Published: March 1, 2025

Language: Английский

Citations

0
Creating coveted bioluminescence colors for simultaneous myriad-color bioimaging DOI Creative Commons
Mitsuru Hattori, Tetsuichi Wazawa,

Mariko Orioka

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Abstract Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of bioluminescent proteins must sufficiently vary enable simultaneous detection. Here, we aimed introduce method can be used expand color variation tuning dual-acceptor bioluminescence resonance energy transfer (BRET). Using this approach, could visualize targets with up 20 through single-shot acquisition using CMOS camera. Overall, enables simple and observation numerous biological phenomena.

Language: Английский

Citations

0