International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(13), P. 11088 - 11088
Published: July 4, 2023
Danshen
has
been
widely
used
for
the
treatment
of
central
nervous
system
diseases.
We
investigated
effect
dihydroisotanshinone
I
(DT),
a
compound
extracted
from
Danshen,
as
well
corresponding
mechanisms
in
an
vitro-based
6-OHDA-induced
Parkinson's
disease
(PD)
model.
SH-SY5Y
human
neuroblastoma
cell
lines
were
pretreated
with
6-hydroxydopamine
(6-OHDA)
and
challenged
DT.
Subsequently,
viability
levels
reactive
oxygen
species
(ROS)
caspase-3
analyzed.
The
DT
on
6-OHDA-treated
cells
expression
core
circadian
clock
genes
measured
using
real-time
quantitative
polymerase
chain
reaction.
Our
results
indicated
that
attenuated
death
suppressed
ROS
caspase-3.
Moreover,
reversed
both
RNA
protein
BMAL1
SIRT1
cells.
Additionally,
inhibitor
reduced
viability.
activators
activated
BMAL1,
then
damaged
by
6-OHDA.
silencing
was
enhanced
resulted
downregulation
reduction
In
conclusion,
our
investigation
suggested
reduces
apoptosis,
including
antioxidative
due
to
ROS,
regulates
enhancing
suppressing
BMAL1.
may
possess
novel
therapeutic
potential
PD
future,
but
further
vivo
studies
are
still
needed.
Polysaccharides,
Journal Year:
2024,
Volume and Issue:
5(3), P. 380 - 398
Published: Aug. 5, 2024
Gel
electrophoresis
and
size
exclusion
chromatography
(SEC)
are
vital
techniques
in
biochemical
research,
employing
gel
matrix
structures
made
of
polysaccharides
or
synthetic
polymers
like
polyacrylamide
for
the
analysis
separation
macromolecules.
Polysaccharides,
such
as
agarose,
offer
safer
alternatives
to
acrylamide.
Polysaccharide
gels,
notably
facilitate
purification
proteins
nucleic
acids
through
a
molecular
sieving
mechanism.
is
mainly
divided
into
denaturing
native
methods.
Denaturing
with
sodium
dodecyl
sulfate
(SDS)
simplifies
protein
migration
but
disrupts
interactions.
Conversely,
electrophoresis,
without
SDS,
allows
migrate
based
on
running
pH
isoelectric
point
proteins,
while
consistently
toward
anode.
The
variable
charges
presents
complexes.
This
review
focuses
use
polysaccharides,
particularly
highlighting
their
applications
separating
It
also
discusses
limitations
agarose
gels
when
used
electrophoresis.
Such
information
should
help
designing
experiments
using
polysaccharides.
Frontiers in Oncology,
Journal Year:
2023,
Volume and Issue:
13
Published: May 1, 2023
Sarcoma
is
a
rare
and
complex
disease
comprising
over
80
malignant
subtypes
that
frequently
characterized
by
poor
prognosis.
Challenges
in
clinical
management
include
uncertainties
diagnosis
classification,
limited
prognostic
predictive
biomarkers,
incompletely
understood
heterogeneity
among
within
subtypes,
lack
of
effective
treatment
options,
progress
identifying
new
drug
targets
novel
therapeutics.
Proteomics
refers
to
the
study
entire
complement
proteins
expressed
specific
cells
or
tissues.
Advances
proteomics
have
included
development
quantitative
mass
spectrometry
(MS)-based
technologies
which
enable
analysis
large
numbers
with
relatively
high
throughput,
enabling
be
studied
on
scale
has
not
previously
been
possible.
Cellular
function
determined
levels
various
their
interactions,
so
offers
possibility
insights
into
cancer
biology.
therefore
potential
address
some
key
current
challenges
described
above,
but
it
still
its
infancy.
This
review
covers
proteomic
sarcoma
studies
findings
pertain
utility.
Proteomic
methodologies
applied
human
research
are
briefly
described,
including
recent
advances
MS-based
technology.
We
highlight
illustrate
how
may
aid
improve
classification
distinguishing
histologies
identify
distinct
profiles
histological
understanding
heterogeneity.
also
where
prognostic,
therapeutic
biomarkers.
These
traverse
range
chordoma,
Ewing
sarcoma,
gastrointestinal
stromal
tumors,
leiomyosarcoma,
liposarcoma,
peripheral
nerve
sheath
myxofibrosarcoma,
rhabdomyosarcoma,
synovial
osteosarcoma,
undifferentiated
pleomorphic
sarcoma.
Critical
questions
unmet
needs
can
potentially
addressed
outlined.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(13), P. 11088 - 11088
Published: July 4, 2023
Danshen
has
been
widely
used
for
the
treatment
of
central
nervous
system
diseases.
We
investigated
effect
dihydroisotanshinone
I
(DT),
a
compound
extracted
from
Danshen,
as
well
corresponding
mechanisms
in
an
vitro-based
6-OHDA-induced
Parkinson's
disease
(PD)
model.
SH-SY5Y
human
neuroblastoma
cell
lines
were
pretreated
with
6-hydroxydopamine
(6-OHDA)
and
challenged
DT.
Subsequently,
viability
levels
reactive
oxygen
species
(ROS)
caspase-3
analyzed.
The
DT
on
6-OHDA-treated
cells
expression
core
circadian
clock
genes
measured
using
real-time
quantitative
polymerase
chain
reaction.
Our
results
indicated
that
attenuated
death
suppressed
ROS
caspase-3.
Moreover,
reversed
both
RNA
protein
BMAL1
SIRT1
cells.
Additionally,
inhibitor
reduced
viability.
activators
activated
BMAL1,
then
damaged
by
6-OHDA.
silencing
was
enhanced
resulted
downregulation
reduction
In
conclusion,
our
investigation
suggested
reduces
apoptosis,
including
antioxidative
due
to
ROS,
regulates
enhancing
suppressing
BMAL1.
may
possess
novel
therapeutic
potential
PD
future,
but
further
vivo
studies
are
still
needed.
