Insights into MLH1 Methylation in Endometrial Adenocarcinoma through Pyrosequencing Analysis: A Retrospective Observational Study DOI Open Access
Fábio França Vieira e Silva, Andrea Ballini, Vito Carlo Alberto Caponio

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(11), P. 2119 - 2119

Published: June 1, 2024

Background: In cancer care, the MLH1 gene is crucial for DNA mismatch repair (MMR), serving as a vital tumor suppressor. Evaluating protein expression status, followed by analysis of promoter methylation, has become key diagnostic and prognostic approach. Our study investigates complex link between methylation prognosis in endometrial adenocarcinoma (EA) patients. Methodology: status was accessed Pyrosequencing (PSQ) assay. Qualitative positivity established if it exceeded 11% cut-off; well, quantitative conducted to establish correlations with clinicopathological data, relapse-free survival, disease-free survival. Results: revealed that 33.3% patients without experienced relapses, surpassing 23.3% methylation. Furthermore, 16.7% succumbed death, slightly higher rate 17.6% methylated comparisons highlighted mean experiencing relapse 35.8%, whereas those relapse, 42.2%. This pattern persisted disease-specific survival (DSS), where deceased exhibited level 49.1% compared living 38.8%. Conclusions: findings emphasize efficacy PSQ evaluating While unmethylation appears be associated rate, does not seem influenced Quantitative percentages suggest elevated linked mortality, though larger sample size would essential statistically significant results.

Language: Английский

Landscape of Endometrial Cancer: Molecular Mechanisms, Biomarkers, and Target Therapy DOI Open Access

Ioana-Stefania Bostan,

Mirela Mihăilă, Viviana Roman

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(11), P. 2027 - 2027

Published: May 27, 2024

Endometrial cancer is one the most prevalent gynecological cancers and, unfortunately, has a poor prognosis due to low response rates traditional treatments. However, progress in molecular biology and understanding genetic mechanisms involved tumor processes offers valuable information that led current classification describes four subtypes of endometrial cancer. This review focuses on pathogenesis cancers, such as mutations, defects DNA mismatch repair pathway, epigenetic changes, or dysregulation angiogenic hormonal signaling pathways. The preclinical genomic investigations presented allowed for identification some molecules could be used biomarkers diagnose, predict, monitor progression Besides therapies known clinical practice, targeted therapy described new treatment involves identifying specific targets cells. By selectively inhibiting these targets, key pathways can disrupted while normal cells are protected. connection between vital fight against Ongoing research trials exploring use standard agents combination with other strategies like immunotherapy anti-angiogenesis improve outcomes personalize patients approach potential transform management patients. In conclusion, enhancing tools essential stratifying risk guiding surgery, adjuvant therapy, women addition, from this may have an value personalized patient’s life.

Language: Английский

Citations

5
Role of Folate Receptor and CD44 in Targeting of Docetaxel and Paclitaxel Fabricated Conjugates for Efficient Cancer Therapy DOI Creative Commons
Amol Tatode, Pranav Agrawal, Jayshree Taksande

et al.

Journal of Medicine Surgery and Public Health, Journal Year: 2024, Volume and Issue: unknown, P. 100163 - 100163

Published: Dec. 1, 2024

Language: Английский

Citations

4
Clinical translation of metabolomics markers in endometrial carcinoma DOI
Nur Fatin Nabilah Mohd Sahardi,

M Vishnu Priya,

Suzana Makpol

et al.

Journal of Obstetrics and Gynaecology Research, Journal Year: 2025, Volume and Issue: 51(3)

Published: Feb. 27, 2025

Abstract Objective This comprehensive review highlights the current research on metabolomics and metabolic pathways involved in endometrial cancer (EC), offering potential non‐invasive biomarkers for EC. Methods The data was extracted from published manuscripts between 2015 2024 using reputed search engine “Pubmed.” All gathered were organized into a single table, facilitating comparison with earlier findings. Results results of this study revealed most metabolites identified previous metabolomic EC are associated lipid, glucose, amino acid metabolism. Conclusion Therefore, understanding these pathway alterations is crucial improving diagnosis, prognosis, treatments by specially targeting pathways.

Language: Английский

Citations

0
Insights into MLH1 Methylation in Endometrial Adenocarcinoma through Pyrosequencing Analysis: A Retrospective Observational Study DOI Open Access
Fábio França Vieira e Silva, Andrea Ballini, Vito Carlo Alberto Caponio

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(11), P. 2119 - 2119

Published: June 1, 2024

Background: In cancer care, the MLH1 gene is crucial for DNA mismatch repair (MMR), serving as a vital tumor suppressor. Evaluating protein expression status, followed by analysis of promoter methylation, has become key diagnostic and prognostic approach. Our study investigates complex link between methylation prognosis in endometrial adenocarcinoma (EA) patients. Methodology: status was accessed Pyrosequencing (PSQ) assay. Qualitative positivity established if it exceeded 11% cut-off; well, quantitative conducted to establish correlations with clinicopathological data, relapse-free survival, disease-free survival. Results: revealed that 33.3% patients without experienced relapses, surpassing 23.3% methylation. Furthermore, 16.7% succumbed death, slightly higher rate 17.6% methylated comparisons highlighted mean experiencing relapse 35.8%, whereas those relapse, 42.2%. This pattern persisted disease-specific survival (DSS), where deceased exhibited level 49.1% compared living 38.8%. Conclusions: findings emphasize efficacy PSQ evaluating While unmethylation appears be associated rate, does not seem influenced Quantitative percentages suggest elevated linked mortality, though larger sample size would essential statistically significant results.

Language: Английский

Citations

1