Cited by Advancements, challenges, and future perspectives in developing feline herpesvirus 1 as a vaccine vector

Advances in CRISPR-Cas technology and its applications: revolutionising precision medicine DOI

Sarkar Sardar Azeez,

Rahin Shareef Hamad,

Bahra Kakamin Hamad

et al.

Frontiers in Genome Editing, Journal Year: 2024, Volume and Issue: 6

Published: Dec. 12, 2024

CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-associated proteins) has undergone marked advancements since its discovery as an adaptive immune system in bacteria and archaea, emerged a potent gene-editing tool after the successful engineering of synthetic guide RNA (sgRNA) toward targeting specific DNA sequences with high accuracy. Besides editing ability, further-developed Cas variants can also edit epigenome, rendering versatile for genome epigenome manipulation pioneering force precision medicine. This review explores latest technology therapeutic biomedical applications, highlighting transformative impact on Moreover, current status CRISPR therapeutics clinical trials is discussed. Finally, we address persisting challenges prospects technology.

Language: Английский

Citations

Overcoming CRISPR-Cas9 off-target prediction hurdles: A novel approach with ESB rebalancing strategy and CRISPR-MCA model DOI

Yanpeng Yang, Yanyi Zheng, Quan Zou

et al.

PLoS Computational Biology, Journal Year: 2024, Volume and Issue: 20(9), P. e1012340 - e1012340

Published: Sept. 3, 2024

The off-target activities within the CRISPR-Cas9 system remains a formidable barrier to its broader application and development. Recent advancements have highlighted potential of deep learning models in predicting these effects, yet they encounter significant hurdles including imbalances datasets intricacies associated with encoding schemes model architectures. To surmount challenges, our study innovatively introduces an Efficiency Specificity-Based (ESB) class rebalancing strategy, specifically devised for featuring mismatches-only instances, marking pioneering approach this realm. Furthermore, through meticulous evaluation various One-hot alongside numerous hybrid neural network models, we discern that moderate complexity ideally balance performance efficiency. On foundation, advance novel model, CRISPR-MCA, which capitalizes on multi-feature extraction enhance predictive accuracy. empirical results affirm ESB strategy surpasses five conventional methods addressing extreme dataset imbalances, demonstrating superior efficacy applicability across diverse models. Notably, CRISPR-MCA excels effect prediction four distinct significantly outperforms contemporary state-of-the-art comprising both mismatches indels. In summation, coupled offers profound insights robust framework future explorations field.

Language: Английский

Citations

Integrating CRISPR Technology with Exosomes: Revolutionizing Gene Delivery Systems DOI

Mahintaj Dara, Mehdi Dianatpour, Negar Azarpira

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 740, P. 151002 - 151002

Published: Nov. 17, 2024

Language: Английский

Citations

CRISPR challenges in clinical developments DOI

Mohadeseh Khoshandam, Hossein Soltaninejad,

Iman Bhia

et al.

Progress in molecular biology and translational science, Journal Year: 2024, Volume and Issue: unknown, P. 263 - 279

Published: Aug. 15, 2024

Language: Английский

Citations

Advancements, challenges, and future perspectives in developing feline herpesvirus 1 as a vaccine vector DOI

Xinru Luo,

Ruiying Liang,

Lin Liang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 12, 2024

As the most prevalent companion animal, cats are threatened by numerous infectious diseases and carry zoonotic pathogens such as

Language: Английский

Citations