Revealing the Mechanism of Esculin in Treating Renal Cell Carcinoma Based on Network Pharmacology and Experimental Validation

Biomolecules, Journal Year: 2024, Volume and Issue: 14(8), P. 1043 - 1043

Published: Aug. 22, 2024

Purpose: This study aims to explore the potential mechanisms of esculin in treatment renal cell carcinoma (RCC). Methods: We employed network pharmacology predict and targets RCC. Molecular docking techniques were then validate predicted targets. Additionally, a series vitro experiments conducted verify anticancer effects on RCC cells, including CCK-8 assay, EdU wound healing apoptosis Western blot. Results: Network molecular results identified GAPDH, TNF, GSK3B, CCND1, MCL1, IL2, CDK2 as core GO KEGG analyses suggested that may influence apoptotic processes target PI3K/Akt pathway Furthermore, assay demonstrated inhibited viability. Microscopic observations revealed following treatment, there was an increase crumpling, reduction density, accumulation floating dead cells. with increasing concentrations, proportion EdU-positive cells decreased, closure ratio PI-positive increased, expression levels BAX cleaved-caspase-3 proteins level Bcl2 protein decreased. These findings inhibits proliferation migration while promoting apoptosis. Moreover, found GAPDH inhibit pathway. Conclusions: is first elucidate therapeutic The provide evidence supporting clinical application introduce promising new candidate for treatment.

Language: Английский

Potential mechanisms of ZiGongDing in treating HPV-induced cervical intraepithelial neoplasia: a network pharmacology and experimental verification study

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Language: Английский

Ebeiedinone and peimisine inhibit cigarette smoke extract-induced oxidative stress injury and apoptosis in BEAS-2B cells

Cell Stress and Chaperones, Journal Year: 2024, Volume and Issue: 29(6), P. 697 - 708

Published: Oct. 4, 2024

Language: Английский