First Isolation of the Heteropathotype Shiga Toxin-Producing and Extra-Intestinal Pathogenic (STEC-ExPEC) E. coli O80:H2 in French Healthy Cattle: Genomic Characterization and Phylogenetic Position

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5428 - 5428

Published: May 16, 2024

The emerging heteropathotype shigatoxigenic (STEC) and extra-intestinal pathogenic Escherichia coli (ExPEC) O80:H2 has been the second leading cause of pediatric HUS in France since mid-2010s. In contrast with other highly STEC serotypes, for which ruminants have clearly identified as main human infection source, this heteropathotype’s reservoir remains unknown. context, we describe first time isolation seven strains from healthy cattle on a single farm France. This study aimed at (i) characterizing genome (ii) investigating phylogenetic positions these strains. virulomes, resistomes, bovine isolates were investigated using silico typing tools, antimicrobial susceptibility testing cgMLST analysis after short-read whole sequencing (WGS). One representative isolate (A13P112V1) was also subjected to long-read sequencing. possessed ExPEC-related virulence genes pR444_A-like mosaic plasmid, previously described strain RDEx444 known confer multi-drug resistance. All clonally related clustered clinical Switzerland range locus differences only one five. conclusion, our findings suggest that could potentially act STEC-ExPEC pathotype.

Language: Английский

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Occurrence of Extended-spectrum β-lactamase (ESBL) and Carbapenemase-producing Escherichia coli isolated from Childhood Diarrhoea in Yaoundé, Cameroon

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 10, 2024

Extended-spectrum β-lactamase (ESBL)-producing pathogenic E. coli is a global public health issue, especially in sub-Saharan African countries such as Cameroon. It contributes to increase significantly hospital length of stay, morbidity, mortality and economic costs because treatment failures. This study aims at determining the resistance background virulence profiles ESBL-E. isolates among childhood diarrhoea during cholera outbreak occuring Yaoundé,

Language: Английский

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Risk factors for diarrheagenic Escherichia coli infection in children aged 6–24 months in peri-urban community, Nairobi, Kenya

PLOS Global Public Health, Journal Year: 2023, Volume and Issue: 3(11), P. e0002594 - e0002594

Published: Nov. 22, 2023

Escherichia coli commonly inhabits the gut of humans and animals as part their microbiota. Though mostly innocuous, some strains have virulence markers that make them pathogenic. This paper presents results a cross-sectional epidemiological study examining prevalence diarrheagenic E . (DEC) pathotypes in stool samples asymptomatic healthy children (n = 540) Dagoretti South subcounty, Nairobi, Kenya. was cultured pathotyped using PCR to target specific associated with Shiga-toxin, enteropathogenic, enterotoxigenic, enteroaggregative, entero-invasive diffusely adherent Overall DEC 20.9% (113/540) enteropathogenic being most prevalent (34.1%), followed by enteroaggregative (23.5%) Shiga-toxin producing (22.0%) among positive samples. We found evidence co-infection multiple 15% Our models indicated at household level, carriage age group [12–18 months] (OR 1.78; 95%CI 1.03–3.07; p 0.04), eating matoke (mashed bananas) 2.32; 1.44–3.73; 0.001) pulses/legumes 1.74; 1.01–2.99; 0.046) while livestock ownership or contact showed no significant association (p>0.05). findings revealed pathogenic circulating presumptive community. Since there has been previous an between any food type carriage, unhygienic handling, preparation could be reason for carriage. Children 12–18 months old are more prone infections due exploration hand-to-mouth behavior. A detailed understanding is required on what proportion cases developed severe symptomatology well fatal outcomes. The rapidly urbanizing environment needs investigated hybrid hetero-pathotype circulation implicated infection outbreaks.

Language: Английский

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Ensuring safe drinking water: microbial evaluation of restaurants in Patuakhali district, Bangladesh

Asian-Australasian Journal of Food Safety and Security, Journal Year: 2024, Volume and Issue: 8(1), P. 5 - 12

Published: April 5, 2024

Drinking water (DW) from restaurants is a major source of microbial exposure among consumers that causes diseases. The evaluation the microbiological quality drinking in provides foundation for appropriate action to minimize contamination and protect patrons food-borne illnesses. aim this study was determine restaurant’s water. A total 20 samples were collected sterile test tube Dumki Patuakhali Sadar Upazilla district. Total viable count on Nutrient agar coliform MacConkey done by standard plate (SPC) technique bacterial identification different biochemical tests. present found all (100%) contaminated with bacteria ranges 4.50 × 104 7.00 x 105, 95% E. coli, range 0 2.8 102. 26 distinct colony isolated various morphologically characterized. Based characteristics, we identified 10 species. Among them, 6 (23.10%) both Enterobacter aerogenes Staphylococcus epidermidis followed Escherichia coli (E. coli) 5 (19.23%), Pseudomonas spp. 3(11.54%), rest Vibrio cholera, Klebsiella oxytoca, S. auerous, Bacillus spp., Aeromonas salmonicida Salmonella 1 (3.85%) each. diverse fecal coliform, enteropathogenic pathogenic indicates unsanitary handling storage practices restaurants; could make customers more susceptible illnesses ailments linked Asian Australas. J. Food Saf. Secur. 2024, 8(1), 5-12

Language: Английский

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Study of plasmid mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from pediatric patients with sepsis

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 24, 2024

Abstract The resistance to antibiotics in Gram-negative bacilli causing sepsis is a warning sign of failure therapy. Klebsiella pneumoniae ( K . ) and Escherichia coli E. represent major associated with sepsis. Quinolone an emerging among K. Therefore, the present study aimed presence plasmid-mediated quinolone PMQR genes qnrA, qnrB, qnrS by polymerase chain reaction (PCR) isolated from pediatric patients This was retrospective cross-sectional that included healthcare-associated isolates were identified microbiological methods. namely detected E PCR. results analyzed SPPS24, qualitative data as numbers percentages comparison performed Chi-square test, P significant if < 0.05. most prevalent gene PCR qnrA (75%), followed qnrB (28.1%), (25%). frequently qnr (28.8%, 16.3% respectively). highlights high prevalence ciprofloxacin There frequency patients. it important monitor spread clinical ensure efficient antibiotic use those children. finding denotes importance surveillance program.

Language: Английский

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Molecular Characterization and Antibiotic Resistance Profile ofEscherichia coliIsolated From Liver Abscess

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 23, 2024

Abstract Background Bacterial liver abscess is the most common hepatic infection, which can lead to death. Escherichia coli among many species of bacteria that cause it. This study was conducted isolate E. from and then characterise bacteria’s molecular makeup antibiotic resistance profile. Methods A total 208 stool samples were collected patients showing symptoms abscess. isolated these followed by identification biochemical tests. Pure biochemically positive colonies confirmed polymerase chain reaction. The disk diffusion method used ascertain pattern exhibited isolates. Results PCR amplification efficiency nearly 100% since all appeared at 284 base pairs (bp), considered be optimal parameter assay. antimicrobial susceptibility showed isolates resistant drugs but 92% susceptible imipenem azithromycin, respectively. All ampicillin, vancomycin, cefotaxime. ceftazidime (72%), tetracycline (84%), trimethoprim (80%), streptomycin (96%), linezolid (92%), Teicoplanin nalidixic acid ciprofloxacin chloramphenicol (72%). Conclusion Multiple drug one causes abscesses in humans.

Language: Английский

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Effect of Wastewater Irrigation on the Presence of Extended Spectrum Beta-Lactamase (Esbl) Producing Escherichia Coli in Agricultural Soil

Published: Jan. 1, 2024

Language: Английский

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Occurrence of extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Escherichia coli isolated from childhood diarrhoea in Yaoundé, Cameroon

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Introduction: Extended-spectrum β-lactamase (ESBL)-producing pathogenic E. coli is a global public health issue, especially in sub-Saharan African countries such as Cameroon. It contributes to increase hospital length of stay, morbidity, mortality, and economic costs because treatment failures. This study aims at determining the resistance virulence profiles ESBL-E. among childhood diarrhoea during cholera outbreak Yaoundé, Materials Methods: During four-month period from March 1st June 30^th, 2023, stool samples were collected 90 under five children presenting clinical signs gastroenteritis attending four hospitals Bacterial identification was done using API20E antimicrobial susceptibility test performed Kirby-Bauer disc diffusion method. After extraction, genomic DNA subjected conventional multiplex polymerase chain reaction (PCR) for detection genes. Statistical analysis Epi info^TM (7.2.5.0). significance considered p-value <0.05. Results: Out 150 patients contacted, enrolled, 84 collected, 52.38%(44/84) 3.57%(03/84) confirmed extended-spectrum carbapenemase-producing respectively. The risk factors analyzed, who drank natural fruit juice (OR: 0.4, p-value: 0.03) found be significantly associated with ESBL-Ec. ESBL-Ec isolates showed high level amoxicillin-clavulanic acid, cefotaxime, ceftazidime, cefepime, colistin, tetracycline. bla_CTX-M was more prevalent ß-lactamase gene. tetracycline genes tet(A) tet(B) also detected. most important detected FimH (81.81%) papA (79.54%). Conclusion: These findings suggest implementing routine surveillance screening resistance. Antimicrobial stewardship strategies (ASP) need implemented curb emergence dissemination In addition, national program needs local regional levels order reduce morbidity

Language: Английский

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Molecular characterization of multidrug-resistant (MDR) Escherichia coli in the Greater Accra Region, Ghana: a ‘One Health’ approach.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 25, 2024

Background: ‘One health’, a concept that highlights the need to bring on board multiple players and actors together addressing major health problems, have been proposed be effective in data gathering mitigate menace of antimicrobial drug resistance (AMR). Data MDR extended-spectrum-beta-lactamase-producingEscherichia coli(ESBL-EC) across humans, animals environment are limited low-and-middle-income-countries (LMICs) including Ghana.Objective: This study used one approach determine prevalence, antibiogram AMR genes ESBL-ECfrom diverse sources.Methodology: A cross-sectional conducted Accra Tema metropolis, Ghana. We randomly collected 1500 non-duplicated specimens from healthy human, cattle, pigs, lettuce, spring onions, pork, beef soil samples.Escherichia coli(E. coli)was isolated confirmed by MALDI-TOF MS.E. coliisolates were screened for their susceptibility against 13 antibiotic agents ESBL-production. ESBL-ECisolates whole-genome sequenced (WGS) andin silicoanalysis was genes, sequence types (STs) plasmid replicon types.Result: Overall,E. coliwas recovered 140 (9.3%) processed. About one-third theseE. 50 (35.7%) resistant three or more antibiotics, 30 (21.5%) ESBL-EC. The proportion ESBL-ECidentified pig, 14 (20.0%), 9 (22.5%), 3 (15.0%), 1 (50.0%) (37.5%), respectively. NoE. onions pork. Overall, ESBL-ECexhibited high levels ampicillin (100%), cefuroxime ciprofloxacin (53.6%), tetracycline (58.2%). However, all sensitive meropenem. prevalent detected wereblaTEM-1B (32.0%; n=8),tetA (48.0%; n=12) andsul2(32.0%; n=8). dominant STs ST10 (12%; n=3), ST 9312 206 n=3) 4151 n=3). IncFIB(Apoo1918) (40.0%; n=10) IncFII(pCoo) (36.0%; n=9) replicons commonly detected.Conclusion: Within metropolis surveyed, we identified ESBL-ECharbouring various with diverseE. coliSTs environment. finding ofblaCTX-M-15 agricultural isolate is worrisome, emphasizing one-health combating AMR.

Language: Английский

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El «segundo enterramiento» de los represaliados del franquismo: dispositivo sacrificial, víctima y liminaridad ritual

Disparidades Revista de Antropología, Journal Year: 2024, Volume and Issue: 79(1), P. 947 - 947

Published: July 10, 2024

En este texto se tratan los proyectos familiares de búsqueda, exhumación y reinhumación restos personas desaparecidas durante la represión franquista como una práctica ritual funeraria semejante a un «segundo enterramiento». Un aspecto central es situación liminaridad en que permanecen desaparecidos sus familiares, imposibilitando clausura del duelo. Ello influye las relaciones filiación familiar con antepasados violentamente, así modos reconocimiento e inclusión calidad víctimas espacios representación social más amplios. Sostengo el argumento categoría «desaparecido» espacio acción jurídica política pero, vez, también forma capturar reivindicaciones colectivos memorialistas modo acto reclamar derechos quedan neutralizados políticamente.

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