NOSOCOMIAL KLEBSIELLA PNEUMONIAE INFECTION IN A CARDIAC SURGERY HOSPITAL: CLINICAL AND MICROBIOLOGICAL FEATURES DOI Creative Commons
М. В. Кузнецова, Veronika S. Mihailovskaya,

L. G. Kudryavtseva

et al.

Russian Journal of Infection and Immunity, Journal Year: 2024, Volume and Issue: 14(6), P. 1117 - 1130

Published: Dec. 9, 2024

Klebsiella pneumoniae dominates among the pathogens that cause healthcare-associated infections and closely associated with high mortality of patients undergoing heart surgery. K. spp. are classified into classical (cKp) hypervirulent (hvKp): cKp causing nosocomial often exerting a multidrug resistance phenotype (MDR-cKp), whereas hvKp sensitive to antibiotics, but have virulence. Earlier, we presented molecular genetic characteristics bacteria isolated from at cardiac surgical hospital. Here, clinical microbiological risk factors as well thoase underlying infection development adverse outcome caused by were evaluated. Along initially severe comorbidities, most significant following: female gender, age over 65 years, length stay Department Anesthesiology Intensive Care other detected comorbidities. The average was 1.7 times longer MDR-cKp vs patients. Complications (multiple organ dysfunction syndrome, bloodstream infections), rate recorded only in patient group. Thus, hospital period, rather than hypervirulence turned out be more contributing factor. It should noted one convergent MDR-hvKp isolate has been detected, which may pose serious problem treatment upon further spread such bacteria.

Language: Английский

Hypervirulent Klebsiella pneumoniae: Epidemiology outside Asian countries, antibiotic resistance association, methods of detection and clinical management DOI Creative Commons
Silvia García-Cobos, Jesús Calatayud, Marı́a Pérez-Vázquez

et al.

Enfermedades Infecciosas y Microbiología Clínica, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Pathogenic analysis of Andinoacara rivulatus skin ulcer disease caused by Klebsiella pneumoniae in China DOI
Xuezheng Shi,

Senting Pu,

Yihao Li

et al.

Aquaculture International, Journal Year: 2025, Volume and Issue: 33(2)

Published: Jan. 24, 2025

Language: Английский

Citations

0
Systematic review and meta-analysis on the carbapenem-resistant hypervirulent Klebsiella pneumoniae isolates DOI Creative Commons

Motahareh Sabaghi Qala Nou,

Zahra Amirian, Fatemeh Dehghani

et al.

BMC Pharmacology and Toxicology, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 30, 2025

The global dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) poses a critical threat to public health. However, comprehensive data on the prevalence and resistance rates CR-hvKp are limited. This systematic review meta-analysis aim estimate pooled carbapenem among hvKp strains assess distribution carbapenemase genes. A search ISI Web Science, PubMed, Google Scholar was conducted identify studies reporting in strains. genes calculated using event with 95% confidence intervals. total 36 encompassing 1,098 were included. 49% for imipenem, 53.2% meropenem, 38.2% ertapenem. Carbapenemase gene 19.1% blaVIM, 22.0% blaNDM, 43.4% blaOXA-48, 58.8% blaKPC. high widespread underscore their significant These findings highlight urgent need enhanced surveillance, rapid diagnostic tools, stringent infection control measures mitigate spread CR-hvKp. Future research should focus understanding mechanisms developing targeted therapeutic strategies address this challenge.

Language: Английский

Citations

0
Hypervirulent Klebsiella pneumoniae: Epidemiology outside Asian countries, antibiotic resistance association, methods of detection and clinical management DOI Creative Commons
Silvia García-Cobos, Jesús Calatayud, Marı́a Pérez-Vázquez

et al.

Enfermedades infecciosas y microbiologia clinica (English ed ), Journal Year: 2025, Volume and Issue: 43(2), P. 102 - 109

Published: Feb. 1, 2025

Two main Klebsiella pneumoniae pathotypes are of public health concern, classical K. (cKP), with high antibiotic resistance acquisition capacity, and hypervirulent (hvKP). The emergence antibiotic-resistant pneumoniae, especially carbapenem resistance, is worrisome require effective methods for detection treatment. Different evolutionary paths contribute to the hypervirulence commonly via plasmids by hvKP (CR-hvKP) or virulence CRKp (hv-CRKp). ST11-KL64 together blaKPC-2, most extended hv-CRKP lineage acquiring associated biomarkers, rmpA, rmpa2, iroBCDEN, iucABCDiutA, peg344. In addition ST11, other clones have been reported in Europe such as ST101, ST147 ST512, highlighting association OXA-48 NDM carbapenemases. Although still very rare Spain, cases increasing recent years, mainly due ST23-K1, ST380-K2 ST86-K2. Management infections requires active therapy based primarily on susceptibility patters site infection.

Language: Английский

Citations

0
Major Predominant Serotypes and Virulence Genes and Antibiotic Resistance Characteristics of Klebsiella pneumoniae Clinical Isolates in Middle and East China DOI Creative Commons
Wei Pan,

Sui-Ning Chen,

Meijuan Yang

et al.

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 1451 - 1464

Published: March 1, 2025

Klebsiella pneumoniae is a common opportunistic pathogen. Predominant serotypes, virulence genes, and resistance characteristics of K. isolates from patients in different regions China require further investigation. healthy individuals middle east were identified using an auto-bacterial detector. Major serotypes genes the detected by polymerase chain reaction, while drug was determined broth microdilution assays. Respiratory infection observed 70.0% patients. Of patients, 42.3% hypervirulent K (hvKp) which 30.1% 37.0% belonged to K1 K2 with 78.6-87.8% positive rates rmpA rmpA2 genes. The had fewer hvKp rmpA/rmpA2 (7.2% 22.9%/26.5%). Resistance (38.6-79.5%) against 14 antibiotics higher than those (16.4-40.8%). sensitive amikacin (83.6%) polymyxin-B (93.9%) but presented 20.3% 26.6% imipenem meropenem, respectively. urinary infections exhibited resistances (42.1-52%) cefoxitin, cefuroxime, ceftriaxone, ciprofloxacin, levofloxacin respiratory or blood (22.4-39.3%). In K47 K64 multiple (65-90%) all displayed much lower antibiotic (1.9-26.0%). K1/K2 major predominant carbapenem-resistant strains prevalent China. have low resistance, resist cephalosporin/quinolone for treatment bacterial infections. Amikacin can be used treat drug-resistant

Language: Английский

Citations

0
Detection of Klebsiella pneumoniae in Veterinary and Food Matrices Using Loop-Mediated Isothermal Amplification DOI Creative Commons

Icía Bermúdez-Fornos,

Alberto Cepeda, Alejandro Garrido‐Maestu

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(3), P. 296 - 296

Published: March 19, 2025

Klebsiella pneumoniae is an opportunistic human pathogen of high relevance due to its ability acquire antibiotic resistance. This included, along with Enterococcus faecium, Staphylococcus aureus, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter spp., in the ESKAPE group, which consists most important bacterial pathogens resistant antibiotics clinical setups. Due importance rapid identification infection-causative agents, a novel method for K. was developed present work. based on loop-mediated isothermal amplification (LAMP) evaluated real-time LAMP, as well end-point colorimetric LAMP. Additionally, two different samples, namely, blood urine, food sample, milk; four DNA purification protocols were also (thermal lysis, chelex, magnetic beads, glass milk). The results revealed differences performance LAMP assays depending specific combination matrix–DNA protocol. Overall, protocol reporting best all matrices one it possible reach LOD50 below 10 CFU/mL after short pre-enrichment step 6 h TSB. demonstrated reliability, sensitivity, simplicity could be performed by non-trained personnel thanks format.

Language: Английский

Citations

0
Multidrug-resistant hypervirulent Klebsiella pneumoniae : an evolving superbug DOI
Yuzhong Zheng,

Xiaojue Zhu,

Chao Ding

et al.

Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: March 26, 2025

Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) combines high pathogenicity with multidrug resistance to become a new superbug. MDR-hvKP reports continue emerge, shattering the perception that K. (hvKP) strains are antibiotic sensitive. Patients infected have been reported in Asia, particularly China. Although hvKP can acquire drug genes, seems be more easily transformed from classical (cKP), which has strong gene uptake ability. To better understand biology of MDR-hvKP, this review discusses virulence factors, mechanisms, formation pathways, and identification MDR-hvKP. Given their destructive transmissible potential, continued surveillance these organisms enhanced control measures should prioritized.

Language: Английский

Citations

0
Epidemiological and genomic insights of mcr-1-positive colistin-resistant Klebsiella pneumoniae species complex strains from wastewater treatment plants in Shanghai DOI
Min Jia, Jing Zhang, Jun Feng

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126146 - 126146

Published: March 1, 2025

Language: Английский

Citations

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New insights and perspectives on the virulence of hypervirulent Klebsiella pneumoniae DOI
Ifeanyi Elibe,

Toluwalase Odunayo,

Chibuzor Kenneth Uwazie

et al.

Folia Microbiologica, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Language: Английский

Citations

0
Precise Detection of Carbapenem-Resistant and Hypervirulent Klebsiella pneumoniae Using MOF-Derived Bimetallic Nanocube Hybrid Nanosheet DOI
Dumei Ma, Yongqi Wang,

Jiacheng Ye

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Carbapenem resistance and hypervirulence represent two distinct evolutionary pathways in Klebsiella pneumoniae, posing significant challenges clinical settings. Of particular concern are convergent strains that combine both traits, complicating timely diagnosis treatment. Herein, we present a novel MOF-derived bimetallic nanocube hybrid nanosheet (denoted Pt-G@Cu5Zn8C@Au) designed to enhance laser desorption/ionization mass spectrometry (LDI-MS) distinguishing from other variants. The material, synthesized through the pyrolysis of pristine MOFs, features uniformly distributed Cu Zn synergistic metal sites within carbon matrix, addressing critical limitations current nanomatrices for situ extraction metabolic fingerprints microbial cells, such as limited sensitivity (e.g., amorphous silicon, TiO2, nanoparticles) or relatively weak conductivity stability (MOF-based materials). Utilizing this advanced 248 K. pneumoniae isolates were rapidly extracted, identifying 23 top VIP-score peaks potential biomarkers differentiating their Combined with machine learning, prediction model achieved 100% accuracy carbapenem-sensitive (CS_cKP) hypervirulent (hvKP) using SVM model, while achieving 78.26% them carbapenem-resistant (CR_cKP) KNN/NB models. These findings highlight high efficacy our assay

Language: Английский

Citations

0