Comparative genomic analysis of emerging non-typeable Haemophilus influenzae (NTHi) causing emerging septic arthritis in Atlanta

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19081 - e19081

Published: March 21, 2025

Background Haemophilus influenzae is a Gram-negative bacterium that can exist as commensal organism or cause range of diseases, from ear infections to invasive conditions like meningitis. While encapsulated H. strains have historically been linked severe non-typeable (NTHi) strains, lacking an intact capsule locus, emerged the leading infections, particularly following widespread use serotype b (Hib) vaccine. Methods In response significant increase in NTHi among persons living with HIV metropolitan Atlanta during 2017–2018, we conducted comparative genomic analysis two predominant clones, C1 and C2, identified this period. These clones correspond multilocus sequence types ST164 ST1714, respectively. We analyzed characteristics C2 using whole genome sequencing data compared them broader pangenome identify potential virulence factors genetic adaptations. Results Both isolates were highly related within their clusters, showing maximum 132 SNPs 149 respective core genomes. Genomic revealed deletions known genes, surprisingly suggesting possible attenuation virulence. No unique accessory genes distinguished other although both clusters exhibited consistent loss pxpB gene (encoding 5-oxoprolinase subunit), replaced by mobile cassette containing potentially involved sugar metabolism. All showed enrichment associated systemic infections. Conclusions Our study suggests while possess some markers there are no definitive distinguish these more virulent than strains. The expansion vulnerable population may reflect chance introduction adaptations host environment. Further research needed understand implications findings on clinical management prevention

Language: Английский

---

Efficacy of lavender essential oil against respiratory tract bacteria is influenced by harvesting time

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Antibiotic resistance can be largely associated with the biofilm-forming properties of bacteria, since in this form pathogens show increased to antibiotics and disinfectants. Pathogens, for example airway colonizing Pseudomonas aeruginosa, Streptococcus pneumoniae members genus Haemophilus, are frequently arranged a biofilm on mucous membrane. Essential oils increasingly used both prevention diseases complementary therapies. This study investigated composition antimicrobial activities Lavandula angustifolia Mill. essential oil (LEO) distilled from flowers collected before, during after flowering period. Our results confirm that collection time significantly influences antibacterial activity LEO. The most active sample was LEO before period each experimental setup. resistant pathogen P. aeruginosa (MIC: 2.5 mg/mL), while sensitive S. 0.5 mg/mL). In experiment, too, pre-bloom effective. Its anti-biofilm decreased parallel increasing incubation (4, 6, 8, 12, 24 h). summary, we demonstrated effectiveness LEOs against respiratory biofilms different maturity, exhibited strongest inhibition.

Language: Английский

---

Molecular characteristics and biofilm formation capacity of nontypeable Haemophilus influenza strains isolated from lower respiratory tract in children

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 190, P. 106632 - 106632

Published: March 26, 2024

Language: Английский

---

Lactiplantibacillus plantarum MH-301 reduces Helicobacter pylori treatment-related adverse events via gut-vaginal axis: a randomized, double-blind, placebo-controlled trial

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Background: Adverse events and microbiota dysbiosis resulting from Helicobacter pylori eradication therapy have become increasingly evident. This study investigates the adjunctive therapeutic effects of Lactiplantibacillus plantarum MH-301 in H. therapy, along with its on gut vaginal microbiota. Method: 257 sexually active, premenopausal women aged 18-50 years, diagnosed pyloriinfection alongside chronic gastritis, were randomly allocated to undergo either bismuth-containing quadruple (amoxicillin, furazolidone, potassium bismuth citrate, rabeprazole) combined probiotics (L. MH-301) or a placebo for 14 days. Stool samples swabs collected before after treatment facilitate high-throughput sequencing analysis. Result: The probiotic group exhibited lower incidences bloating (10.2% vs 19.4%, P=0.037), constipation (2.3% 7.8%, P =0.048), excessive discharge (3.1% 9.3%, P=0.040), showed better tolerability compared (P<0.05), although no statistically significant difference eradication rates between two groups. High-throughput revealed that pylori led decrease relative abundance of Prevotella microbiota, an increase Streptococcus Haemophilus. In Lactobacillus decreased while Gardnerellaincreased. Compared group, higher Megamonas, Prevotella, Lactobacillusin levels Gardnerella was significantly (P<0.05). Correlation analysis positive association gastrointestinal adverse events, correlation both Conclusion: is first demonstrate at clinical level L. plantarumMH-301 reduces treatment-related via gut-vaginal axis. addition, modulation could be possible mechanism through which it achieves efficacy.

Language: Английский

---

A Hunt for the Resistance of Haemophilus influnezae to Beta-Lactams

Antibiotics, Journal Year: 2024, Volume and Issue: 13(8), P. 761 - 761

Published: Aug. 12, 2024

Infections due to

Language: Английский

---

Clinical relevance of lung microbiota composition in critically ill children with acute lower respiratory tract infections: insights from a retrospective analysis of metagenomic sequencing

European Journal of Clinical Microbiology & Infectious Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 9, 2024

Acute lower respiratory tract infections (ALRIs) is a leading cause of child mortality worldwide. Metagenomic next-generation sequencing (mNGS) identifies ALRIs pathogens and explores the lung microbiota's role in disease severity clinical outcomes. This study examines association between microbiota outcomes children, exploring its potential as prognostic biomarker.

Language: Английский

---

Antibiofilm Effects of Novel Compounds in Otitis Media Treatment: Systematic Review

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12841 - 12841

Published: Nov. 29, 2024

Otitis media (OM) is a frequent disease with incidence rate of 5300 cases per 100,000 people. Recent studies showed that polymicrobial biofilm formation represents significant pathogenic mechanism in recurrent and chronic forms OM. Biofilm enables bacteria to resist antibiotics would typically be recommended guidelines, contributing the ineffectiveness current antimicrobial strategies. Given challenges successfully treating bacterial biofilms, there an growing interest identifying novel effective compounds overcome antibacterial resistance. The objective this review was provide overview antibiofilm effects on formed by clinical isolates systematic included evaluated effect natural or synthetic from obtained patients eligibility criteria were defined using PICOS system: (P) Population: all human OM; (I) Intervention: compound effect; (C) Control standard therapeutic agents untreated (O) Outcome: (biofilm inhibition, eradication), (S) Study design. PRISMA protocol for reviews meta-analysis followed. From 3564 potentially eligible studies, 1817 duplicates removed, 1705 excluded according exclusion criteria. A total 41 available full texts retrieved two independent authors. Fifteen articles selected inclusion which 125 17 different examined, including N-acetyl-L-cysteine (NAC), tea tree oil, xylitol, eugenol, Aloe barbadensis, Zingiber officinale, Curcuma longa, Acacia arabica, antisense peptide nucleic acids, probiotics Streptococcus salivarius oralis, Sodium 2-mercaptoethanesulfonate (MESNA), bioactive glass, green synthesized copper oxide nanoparticles, radish, silver nanoparticles acetic acid. Staphylococcus aureus most commonly studied pathogen, followed Pseudomonas aeruginosa Haemophilus influenzae. inhibition only examined assessed six studies; eradication four both five studies. This indicates some like NAC, prebiotics, MESNA have are safe could researched more extensively further use. However, lack data about reliable efficient used therapy types otitis still remains literature.

Language: Английский

---

Haemophilus influenzae Invasive Infections in Children in Vaccine Era: Phenotypic and Genotypic Characterization Tunis, Tunisia

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2666 - 2666

Published: Dec. 23, 2024

The changing epidemiological profile of invasive Haemophilus influenzae infections (IIHi) is noted in the post-vaccination era. aim this study was to characterize phenotypically and genotypically (Hi) isolates detected Tunisian pediatric patients. A retrospective conducted microbiology laboratory Children's Hospital Tunis over ten years (2013-2023). All IIHi cases were included. Molecular identification serotyping through qPCR. typing analysis resistance genes extracted from whole genome sequencing data. Fifty-three collected. Children under five old most affected (81%). Non-typable (NTHi) predominant (79%) followed by serotype b (17%) a (4%). Genetic diversity observed, essentially among NTHi isolates. Resistance Hi ampicillin, amoxicillin-clavulanic acid cefotaxime (CTX) 42%, 20% 4%, respectively. Thirteen (29%) produced beta-lactamase 14 carried blaTEM-1 gene (kappa = 0.95). For non-enzymatic resistance, group 3 (n 12) showed ampicillin. Groupe 4 9, NTHi) discordances with CTX. emergence CTX concerning. Continuous surveillance molecular tools conjunction phenotypic clinical data necessary ensure better management these infections.

Language: Английский

---

Evolution of Haemophilus influenzae infection during the vaccination period: literature review

CHILDREN INFECTIONS, Journal Year: 2023, Volume and Issue: 22(4), P. 48 - 57

Published: Nov. 18, 2023

Mass immunization with H. influenzae type b (Hib) vaccines has led to a sharp decrease in the incidence of invasive and non-invasive forms Haemophilus influenza infection. causes diverse spectrum diseases from usually asymptomatic carriage otitis, sinusitis, epiglottitis, pneumonia, endopericarditis, inflammation subcutaneous tissue, arthritis, is one four main causative agents bacterial meningitis. Materials methods . In order identify significance Hemophilus infection during vaccination period, an analysis was carried out on spread infection, its clinical forms, as well antibiotic resistance pathogen. A review domestic foreign publications over past 20 years conducted search engines PubMed, Google Scholar, Cochrane Library Elibrary. Results. Studies various countries show that after start mass immunization, no longer dominant serotype pathogen causing infections most countries. Everywhere world, leading positions are occupied by cases caused non-typable serotypes a, f, (Hia, Hif, Hib) predominate among capsular forms. Unprotected aminopenicillins, second-generation cephalosporins, sulfonamides classes antibacterial drugs which strains have developed resistance. variety still relevant for hemophilic Bacteremia, meningitis, pneumonia manifestations typed non-typed

Language: Английский

---