Daptomycin Liposomes Exhibit Enhanced Activity against Staphylococci Biofilms Compared to Free Drug DOI Creative Commons
Foteini Gkartziou, Maria Plota,

Charikleia Kypraiou

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(4), P. 459 - 459

Published: March 26, 2024

The purpose of the present study was to investigate anti-staphylococcal activity liposomal daptomycin against four biofilm-producing S. aureus and epidermidis clinical strains, three which are methicillin-resistant. Neutral negatively charged daptomycin-loaded liposomes were prepared using methods, namely, thin-film hydration (TFH), a dehydration–rehydration vesicle (DRV) method, microfluidic mixing (MM); moreover, they characterized for drug encapsulation (EE%), size distribution, zeta-potential, stability, release, integrity. Interestingly, whilst loading in THF DRV nanosized (by extrusion) vesicles around 30–35, very low (~4%) possible MM vesicles, requiring further explanatory investigations. Liposomal protected from degradation preserved its bioactivity. Biofilm mass (crystal violet, CV), biofilm viability (MTT), growth curve (GC) assays evaluated antimicrobial neutral daptomycin-liposomes towards planktonic bacteria biofilms. exhibited dramatically enhanced inhibition bacterial (compared free drug) all species studied, while totally inactive. prevention treatment studies revealed high antibiofilm daptomycin. more active negative charge ones treating established Planktonic as well matured biofilms daptomycin-susceptible, methicillin-resistant Staphylococcus (MRSA) (MRSE) strains almost completely eradicated by liposomal-daptomycin, indicating need their exploration therapeutics.

Language: Английский

Pharmacophore-guided computational modeling of quinolone-ATPase conjugate inhibitors targeting DNA GyrB subunit of Staphylococcus aureus DOI
Md. Al Amin,

Md. Jakir Hossain,

Md. Kawsar Habib

et al.

Computers in Biology and Medicine, Journal Year: 2025, Volume and Issue: 186, P. 109727 - 109727

Published: Jan. 25, 2025

Language: Английский

Citations

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An engineered, biocide-grafted TiO2-based nanohybrid material with enhanced photocatalytic and antimicrobial activity DOI Creative Commons
Olga Ferreira, Marvin Katherine Amaya Gil, Ana P. Carapeto

et al.

Journal of Cleaner Production, Journal Year: 2025, Volume and Issue: unknown, P. 144937 - 144937

Published: Feb. 1, 2025

Language: Английский

Citations

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Antimicrobial susceptibility and genetic diversity of Staphylococcus pseudintermedius isolated from companion animals and human clinical patients in Japan: potential zoonotic implications DOI Creative Commons
Masaru Usui,

Rana Fahmi Sabala,

Shigeaki Morita

et al.

Journal of Global Antimicrobial Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

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Decoding Host-Pathogen Interactions in Staphylococcus aureus: Insights into Allelic Variation and Antimicrobial Resistance Prediction Using Artificial Intelligence and Machine Learning based approaches DOI Open Access
Joyeta Ghosh, Jyoti Taneja,

Ravi Kant

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

Abstract This novel study leveraged advanced machine learning techniques to elucidate the molecular mechanisms of antimicrobial resistance (AMR) in 300 Staphylococcus aureus isolates across six critical antibiotics. Employing a diverse array deep and ensemble models, we conducted an in-depth analysis genetic markers allelic variations characterize determinants. Our investigation revealed that XGBoost model demonstrated most exceptional performance, achieving remarkable 95% test accuracy, 100% training unprecedented ROC AUC 0.9855. Comparative multiple approaches, including Long Short-Term Memory (LSTM), Convolutional Neural Network (CNN), Multi-Layer Perceptron (MLP), Decision Tree, Stochastic Gradient Descent (SGD) provided detailed insights into prediction. The SHAP (SHapley Additive exPlanations) unveiled markers, with “cat_allele_Cluster_1015_Allele_8” emerging as influential feature driving predictions. Notably, models exhibited varying performance different antibiotics, consistently high accuracy F1-scores for ciprofloxacin, clindamycin, gentamicin, sulfamethoxazole/trimethoprim. findings not only demonstrate potential predicting but also provide crucial underlying S. drug resistance. By identifying key determinants their relative importance, this offers sophisticated approach understanding patterns, potentially guiding future diagnostic strategies, targeted therapies, stewardship practices clinical settings.

Language: Английский

Citations

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Evaluation of aminoglycoside- and methicillin-resistant Staphylococcus aureus: phenotypic and genotypic insights from clinical specimens in Ardabil, Iran DOI Creative Commons

Samira Hushyar,

Hadi Peeri Doghaheh,

Mohsen Arzanlou

et al.

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 27, 2025

Language: Английский

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Antibacterial and anti-biofilm activities of Derazantinib (ARQ-087) against Staphylococcus aureus DOI
Weiguo Wang, Q.P. Zhong,

Xincheng Huang

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(4)

Published: March 6, 2025

Language: Английский

Citations

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Global prevalence of macrolide-resistant Staphylococcus spp.: a comprehensive systematic review and meta-analysis DOI Creative Commons
Tahereh Navidifar,

Abbas Zare Banadkouki,

Elnaz Parvizi

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 14, 2025

Background Staphylococcus is a genus of bacteria responsible for various infections ranging from mild skin to severe systemic diseases. Methicillin-resistant aureus (MRSA) and coagulase-negative staphylococci (CoNS) are significant challenges owing their resistance multiple antibiotics, including macrolides, such as erythromycin, clarithromycin, azithromycin. Objective This study aimed systematically review synthesize data on the prevalence macrolide in spp., identify trends changes patterns over time, assess how testing methods guidelines affect reported rates. Methods The conducted systematic search Scopus, PubMed, Web Science, EMBASE databases. Studies have proportion macrolide-resistant spp. Two authors independently extracted analyzed using random-effects model. Heterogeneity was assessed, subgroup analyses were performed based country, continent, species, AST guidelines, methods, period. Results In total, 223 studies 76 countries included. pooled azithromycin 57.3, 52.6, 57.9%, respectively. Significant heterogeneity observed across (I 2 > 95%, p < 0.001). Oceania (72%) had highest erythromycin resistance, whereas Europe lowest (40.7%). Subgroup revealed variations with higher MRSA than MSSA CoNS other species. Over slight decrease has been (59.6% 2015–2019 55% 2020–2023). Conclusion emphasizes high its notable regional variation. These findings highlight necessity standardized methodologies global surveillance manage effectively. Controlling antibiotic should prioritize enhancing public health measures updating treatment guidelines. Systematic registration https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=557756 , CRD42024557756.

Language: Английский

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Probing the Multitargeted Potency of FDA-approved Amifostine against MRSA and Control Comparison with Sulfamethoxazole to Establish Alternative Medications DOI
Fawaz M. Almufarriji, Bader S. Alotaibi, Ahlam Saleh Alamri

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107485 - 107485

Published: March 1, 2025

Language: Английский

Citations

0
Biofilm-Mediated Infections; Novel Therapeutic Approaches and Harnessing Artificial Intelligence for Early Detection and Treatment of Biofilm-Associated Infections DOI

Muhammad Bilal Habib,

Ghanwa Batool,

Naseer Ali Shah

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107497 - 107497

Published: March 1, 2025

Language: Английский

Citations

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In Vitro and In Vivo Investigations into the Potential of Quinazoline and Quinoline Derivatives as NorA Efflux Pump Inhibitors Against Resistant Staphylococcus aureus Strains DOI Creative Commons
Nishtha Chandal, Nidhi Sharma, Giada Cernicchi

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(4), P. 339 - 339

Published: March 26, 2025

Background: Staphylococcus aureus is a highly lethal Gram-positive bacterium that responsible for over one million deaths annually. As member of the ESKAPE pathogens, its methicillin-resistant strains (MRSA) are prevalent worldwide and exhibit significant antimicrobial resistance (AMR). Bacterial efflux pumps play pivotal role in development AMR by facilitating expulsion range agents. Methods: The S. strain SA-1199B, which overexpresses NorA carries GrlA mutation, was utilized to comprehensively profile mechanism compounds PQQ16P PQK4F. To assess toxicity genotoxicity these compounds, RAW macrophages, HEK 293T, HepG2 cell lines were utilized. Female BALB/c mice vivo synergism EPIs with CPX, Results: pump inhibitors (EPIs), PQK4F, enhanced efficacy antibacterial ciprofloxacin (CPX) against resistant strains. involved inhibition pump, without compromising bacterial membrane permeability, ATP levels, or mammalian calcium channels. Moreover, significantly augmented bactericidal post-antibiotic effects elevating mutation prevention concentration manifesting substantial human cells. Furthermore, reduced invasiveness indicating virulence. Notably, CPX observed mouse infection model. Conclusions: This study provides evidence potential employing combination counteract AMR, both vitro vivo.

Language: Английский

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