Unveiling environmental transmission risks: comparative analysis of azole resistance in Aspergillus fumigatus clinical and environmental isolates from Yunnan, China DOI Creative Commons

Jianchuan Gong,

Jiarui Huang,

Yongju Liu

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

ABSTRACT Azole resistance in Aspergillus fumigatus poses a significant clinical challenge globally. Our previous epidemiological analysis revealed remarkably high frequency (~80%) of azole-resistant A. Yunnan’s greenhouse environments, prompting increased local and regional research for targeted control strategies. In this study, we analyzed 94 isolates from Yunnan, comparing their susceptibility profiles genotypic characteristics with environmental strains previously isolated. While the overall azole was lower than that samples, prevalence cross-resistance, varying patterns based on minimum inhibitory concentration (MIC) levels observed, which exceeded rates other regions China. Specific mutation combinations cyp51A gene were linked to elevated MIC values and/or while some resistant wild-type remain unexplained, indicating need further investigation into mechanisms. The differences unique genetic elements distinct differentiation observed between can be attributed geomorphology potential genotype importation provinces abroad. Extensive allele exchanges sharing contributed selection isolates, suggesting common origin, transmission routes drug-resistant cannot excluded. These findings emphasize imperative surveillance monitor trends guide effective antifungal therapy, management strategies mitigate invasive aspergillosis risk region. IMPORTANCE is major global health concern, particularly environments. This study compares Yunnan strains, revealing but cross-resistance patterns. mutations associated values, though had , highlighting research. external influences surveillance. Effective monitoring are essential manage aspergillosis.

Language: Английский

Molecular Identification of Aspergillus Species, Antifungal Susceptibility, and Phenotypic Identification of Azole-Resistant Mutations in Cyp51A Gene Isolated from Xinjiang DOI Creative Commons

Aikedai Yusufu,

Zubaidanmu Aizezi,

Xiyidan Nuermaimaiti

et al.

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 1699 - 1711

Published: April 1, 2025

This study aimed to determine the clinical distribution characteristics, in vitro antifungal susceptibility, and cyp51A mutation types of clinically isolated Aspergillus species Xinjiang. In this study, a total 111 were identified by sequencing internal transcribed spacer (ITS) β-tubulin (BenA) genes for molecular identification, performed susceptibility testing on these isolates using Sensititre YeastOne, selected azole-resistant based results amplified gene identification azole resistance phenotype isolates. The most common was A. fumigatus (40.54%), followed niger (18.02%), tubingensis (16.22%), terreus (13.51%), flavus (6.31%), welwitschiae (2.70%), fumigatiaffinis (1.80%), lentulus (0.90%). test showed that fumigatus, niger, tubingensis, completely sensitive itraconazole, with sensitivity rates posaconazole voriconazole 99.10% 88.29%, respectively. rate amphotericin B lowest (62.16%). MIC values two cryptic species, high (>1mg/L). non-susceptible or non-wild type (15/111, 13.51%). Eleven had mutations, while four strains did not have any mutations. pathogenic from cases Xinjiang diverse. Common best activity against posaconazole, echinocandins, whereas significantly higher. Resistant may be mediated point mutations cyp51A, phenotypic are information is great significance guiding early diagnosis therapy aspergillosis.

Language: Английский

Citations

0
Regulation of Ergosterol Biosynthesis in Pathogenic Fungi: Opportunities for Therapeutic Development DOI Creative Commons
Lijiang Song, Sha Wang, Hang Zou

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 862 - 862

Published: April 10, 2025

Ergosterol plays a dual role in fungal pathogenesis and azole resistance, driving key advancements the understanding of its biosynthesis regulation. This review integrates latest research progress on regulation ergosterol drug resistance pathogenicity. We comprehensively discuss functions enzymes (such as Erg11p/Cyp51A, Erg6p, Erg3p, Erg25p) mevalonate, late, alternative pathways. Notably, we highlight complex cyp51A expression by factors such SrbA, AtrR, CBC, HapX, NCT Aspergillus fumigatus, elucidate distinctive roles Upc2, Adr1, Rpn4 Candida species. Importantly, summarize recent discoveries CprA-dependent Cyp51A/Erg11p heme-mediated stability control. Based these findings, propose innovative antifungal strategies, including dual-target inhibition multi-enzyme natural products, which provide novel insights potential directions for development next-generation therapies.

Language: Английский

Citations

0
Antifungal susceptibility of a collection of Aspergillus fumigatus strains isolated from patients with invasive pulmonary aspergillosis and COVID-19 associated aspergillosis DOI Creative Commons
Andrea Liberatore, Giulia Lombardi, Donatella Lombardo

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 113(1), P. 116884 - 116884

Published: May 1, 2025

We assessed the antifungal susceptibility of 86 Aspergillus fumigatus strains by a phenotypic test. Azole sensitivity was compared to molecular test detecting cyp51A mutations. resistance quite limited, whereas from COVID-19 patients showed higher amphotericin B MICs. The 100 %-agreement with assay.

Language: Английский

Citations

0
Epidemiology, antifungal susceptibility and biological characteristics of clinical Aspergillus fumigatus in a tertiary hospital DOI Creative Commons
Yuting Kang,

Wanting Ma,

Qiujie Li

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 15, 2025

Azole resistance in Aspergillus fumigatus poses a significant clinical challenge globally. In this study, we analyzed 307 A. isolates from General Hospital of Ningxia Medical University China, collected between July 2023 and 2024, to explore their susceptibility profiles, genotypic characteristics biological traits. The overall frequency azole among province was found be 1.20% (7 azole-resistant fumigatus, designated AF1-AF7), each exhibiting distinct phenotypes terms resistance, spore viability, resilience environmental stress. Among these strains, the cyp51A mutations predominantly included TR43/L98H, while hmg1 were primarily S212P/Y564H. Additionally, novel discovered cyp51B, specifically t-215c point mutation base deletion (gatgccta) - 213 206 region. AF3 strain demonstrated highest activity anti-SDS efficacy. contrast, AF1 AF2 strains resistant three azoles also exhibited Menadione, similar AF7. Notably, six out seven displayed NaCl KCl, indicating strong tolerance saline conditions. These findings suggest that possess varying degrees stressors, implying they may adapt surroundings through different evolutionary pathways, which could complicate treatment strategies. Effective surveillance control strategies are essential widespread prevalence reduce risk infection patients.

Language: Английский

Citations

0
Antifungal Susceptibility and Genotypic Analysis of cyp51A Mutations in Aspergillus fumigatus Isolates in Malaysia DOI Creative Commons
Xue Ting Tan,

Nurin Nazirah Mokhtar,

Shirley Yi Fen Hii

et al.

Infection and Drug Resistance, Journal Year: 2024, Volume and Issue: Volume 17, P. 2159 - 2168

Published: May 1, 2024

Azole resistance in

Language: Английский

Citations

0
Unveiling environmental transmission risks: comparative analysis of azole resistance in Aspergillus fumigatus clinical and environmental isolates from Yunnan, China DOI Creative Commons

Jianchuan Gong,

Jiarui Huang,

Yongju Liu

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

ABSTRACT Azole resistance in Aspergillus fumigatus poses a significant clinical challenge globally. Our previous epidemiological analysis revealed remarkably high frequency (~80%) of azole-resistant A. Yunnan’s greenhouse environments, prompting increased local and regional research for targeted control strategies. In this study, we analyzed 94 isolates from Yunnan, comparing their susceptibility profiles genotypic characteristics with environmental strains previously isolated. While the overall azole was lower than that samples, prevalence cross-resistance, varying patterns based on minimum inhibitory concentration (MIC) levels observed, which exceeded rates other regions China. Specific mutation combinations cyp51A gene were linked to elevated MIC values and/or while some resistant wild-type remain unexplained, indicating need further investigation into mechanisms. The differences unique genetic elements distinct differentiation observed between can be attributed geomorphology potential genotype importation provinces abroad. Extensive allele exchanges sharing contributed selection isolates, suggesting common origin, transmission routes drug-resistant cannot excluded. These findings emphasize imperative surveillance monitor trends guide effective antifungal therapy, management strategies mitigate invasive aspergillosis risk region. IMPORTANCE is major global health concern, particularly environments. This study compares Yunnan strains, revealing but cross-resistance patterns. mutations associated values, though had , highlighting research. external influences surveillance. Effective monitoring are essential manage aspergillosis.

Language: Английский

Citations

0