Study on collateral sensitivity of tigecycline to colistin-resistant Enterobacter cloacae complex DOI Creative Commons
Kaixin Yu,

Jiming Wu,

Mingjing Liao

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: May 23, 2025

ABSTRACT The past decade has witnessed the emergence and spread of carbapenem-resistant Enterobacter cloacae complex (CRECC), presenting a significant clinical challenge urgently demanding new treatment strategies against antimicrobial resistance (AMR). This study focused on impact tigecycline susceptibility CRECC isolates to colistin collateral sensitivity in CRECC. Under pressure, five clinically isolated strains was converted from sensitivity. These mutants exhibited significantly higher expression acrA, acrB , ramA genes, with mutations ramR gene. Overexpression certain did not alter expression. No were identified lipid A synthesis genes; however, phoQ consistently downregulated, arnA varied among CRECC405-resistant mutants. Low-dose combination therapy outperformed monotherapy efficacy. Overall, observed resistance. overexpression acrB, due mutations, led Inconsistent levels genes affected modification, which turn upregulated Increased associated downregulation IMPORTANCE Antimicrobial (AMR) is escalating faster than our ability manage bacterial infections, antibiotic-resistant bacteria emerging as public health risk. Innovative are needed curb AMR dissemination. Our research following (TGC) resistance, resulting newfound (COL), drug it once resistant. Synergistic suppress growth, offering promising approach combat infections progression through strategic pairing antibiotics complementary sensitivities.

Language: Английский

Emergence of concurrently transmissible mcr-9 and carbapenemase genes in bloodborne colistin-resistant Enterobacter cloacae complex isolated from ICU patients in Kolkata, India DOI Creative Commons
Gourab Halder, Bhaskar Chaudhury,

Priyanka Denny

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

ABSTRACT Colistin resistance in carbapenem-resistant Enterobacter cloacae complex (CR-ECC) infections has grown expeditiously but detecting the underlying mechanism of is often challenging clinical settings. This study, first its kind from India, determined mechanisms and characterized colistin-resistant hospital isolates. Twenty-nine bloodborne CR-ECC isolated ICU patients eight tertiary care hospitals Kolkata, India between 2022 2023 were screened for colistin resistance. The plasmid-encoded mcr -9 gene, acrAB-tolC efflux pump (EP) & phoP/Q, pmr A/B two-component system (TCS) involved examined. In addition, AMR gene profiling molecular subtypes -9-producing isolates also investigated. All study showed to ≥5 antimicrobial classes six (21%) them colistin-resistant. transferable by IncHI2-HI2A plasmid was detected both (67%) colistin-sensitive (4%) bla NDM-5 significantly ( P < 0.05) associated with co-harboring genes. A ≥8-fold increase minimum inhibitory concentration (MIC) observed strain after induction. Overexpression acrA, ramA, soxS , pmrA/B genes found non- wild-type appeared be mediated part an active EP, regulatory TCS. typed into ST932, ST270, ST1997 MLST. Heterogeneity (29 pulsotypes; 48.40% similarity coefficient) among circulating revealed PFGE. Robust monitoring -sensitive strains warranted curb menace nosocomial pathogens. IMPORTANCE Carbapenem-resistant become a global pathogen last few years. Colistin, “last resort antibiotic,” being widely used treatment and, consequently, there been brisk rise necessitated dearth comprehensive investigation India. notorious plasmid-borne mcr-9 along phoP/Q-pmr mediate Interestingly, discovered MIC under pressure. Diverse phylogenetic clones novel sequence types detected. highlights necessity intense conjunction existing epidemic harboring diverse arrays transmissible

Language: Английский

Citations

1
Study on collateral sensitivity of tigecycline to colistin-resistant Enterobacter cloacae complex DOI Creative Commons
Kaixin Yu,

Jiming Wu,

Mingjing Liao

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: May 23, 2025

ABSTRACT The past decade has witnessed the emergence and spread of carbapenem-resistant Enterobacter cloacae complex (CRECC), presenting a significant clinical challenge urgently demanding new treatment strategies against antimicrobial resistance (AMR). This study focused on impact tigecycline susceptibility CRECC isolates to colistin collateral sensitivity in CRECC. Under pressure, five clinically isolated strains was converted from sensitivity. These mutants exhibited significantly higher expression acrA, acrB , ramA genes, with mutations ramR gene. Overexpression certain did not alter expression. No were identified lipid A synthesis genes; however, phoQ consistently downregulated, arnA varied among CRECC405-resistant mutants. Low-dose combination therapy outperformed monotherapy efficacy. Overall, observed resistance. overexpression acrB, due mutations, led Inconsistent levels genes affected modification, which turn upregulated Increased associated downregulation IMPORTANCE Antimicrobial (AMR) is escalating faster than our ability manage bacterial infections, antibiotic-resistant bacteria emerging as public health risk. Innovative are needed curb AMR dissemination. Our research following (TGC) resistance, resulting newfound (COL), drug it once resistant. Synergistic suppress growth, offering promising approach combat infections progression through strategic pairing antibiotics complementary sensitivities.

Language: Английский

Citations

0