Brain neurons internalise polymeric micron-sized capsules: insights from in vitro and in vivo studies

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101493 - 101493

Published: Jan. 22, 2025

Nanoengineered encapsulation presents a promising strategy for targeted drug delivery to specific regions in the body. While polyelectrolyte-based biodegradable microcapsules can achieve highly localised release tissues and cell cultures, delivering drugs intracellular sites brain remains significant challenge. In this study, we utilized advanced imaging techniques, both vitro vivo, investigate whether neurons internalise designed delivery. High-resolution live-cell revealed that differentiating N2A cells actively microcapsules, often incorporating multiple capsules per cell. Likewise, primary hippocampal cortical were observed effectively polymeric microcapsules. intact brain, multiplexed two-photon excitation vivo confirmed internalisation of by following somatosensory region. This was time-dependent, correlated with particle size mediated macropinocytosis mechanism appears bypass lysosomal formation. Importantly, presence internalised did not impair neuronal function, as maintained normal firing activity action potential characteristics. Furthermore, no adverse effects after week microcapsule mouse brain. Our findings indicate are effective safe carriers neurons, providing approach therapeutic applications.

Language: Английский

Enhanced Epithelial Cell Uptake of Glycol Chitosan‐Coated PLGA Nanoparticles for Oral Drug Delivery

Advanced Therapeutics, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract It is reported that poly(lactic‐co‐glycolic acid) (PLGA) nanoparticles (NPs) coated with chitosan and its derivatives, such as glycol (GC), can enhance the targeted uptake of PLGA NPs by intestinal epithelial cells. However, optimal amount GC for coating specific mechanisms which it facilitates endocytosis remain unclear. In this study, PLGA‐NPs are prepared using either single‐ or double‐emulsion methods varying amounts GC. The results confirmed GC‐coated internalized via both clathrin‐mediated caveolae‐mediated endocytosis, whereas uncoated relied on only in Caco‐2 HT‐29 optimized formulation further modified layering alginate to oral delivery insulin. subsequent vivo studies, alginate‐coated demonstrated stability prolonged efficacy, achieving approximately a 50% reduction blood glucose levels at 6 h post‐administration streptozotocin‐induced diabetic mice. These findings provide compelling evidence molecular platform, underscoring feasibility commercial potential platform based GC‐ NPs.

Language: Английский

Biochemical investigation and in silico analysis of the therapeutic efficacy of Ipriflavone through Tet-1 Surface-Modified-PLGA nanoparticles in Streptozotocin-Induced Alzheimer’s like Disease: Reduced oxidative damage and etiological Descriptors

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 669, P. 125021 - 125021

Published: Dec. 2, 2024

Language: Английский