Protective role of extracellular vesicles against oxidative DNA damage DOI Creative Commons
Jordi Ribas‐Maynou, Ana Parra, Pablo Martínez‐Díaz

et al.

Biological Research, Journal Year: 2025, Volume and Issue: 58(1)

Published: March 13, 2025

Abstract Background Oxidative stress, a source of genotoxic damage, is often the underlying mechanism in many functional cell disorders. Extracellular vesicles (EVs) have been shown to be key regulators cellular processes and may involved maintaining redox balance. Herein, we aimed develop method assess effects EVs on DNA oxidation using porcine seminal plasma extracellular (sEVs). Results The technique was set cell-free plasmid avoid bias generated by uptake sEVs sperm cells, employing increasing concentrations hydrogen peroxide (H 2 O ) that generate single-strand breaks (SSBs). Because SSBs contain free 3’-OH end allow extension through quantitative PCR, such -and therefore SYBR intensity- showed proportional amount SSB. In next stage, H co-incubated with two size-differentiated subpopulations (small large) permeabilized non-permeabilized whether intravesicular content (IC) or surface protects from oxidative damage. obtained small reduced incidence ( P < 0.05), whereas large had no impact generation > 0.05). IC protective effect against Conclusions Our results suggest sEVs, including peripheral corona layer, exert function alterations are originated mechanisms. addition, our vitro study opens path detect, localize quantify derived different fluids, elucidating their physiopathological states.

Language: Английский

Protective role of extracellular vesicles against oxidative DNA damage DOI Creative Commons
Jordi Ribas‐Maynou, Ana Parra, Pablo Martínez‐Díaz

et al.

Biological Research, Journal Year: 2025, Volume and Issue: 58(1)

Published: March 13, 2025

Abstract Background Oxidative stress, a source of genotoxic damage, is often the underlying mechanism in many functional cell disorders. Extracellular vesicles (EVs) have been shown to be key regulators cellular processes and may involved maintaining redox balance. Herein, we aimed develop method assess effects EVs on DNA oxidation using porcine seminal plasma extracellular (sEVs). Results The technique was set cell-free plasmid avoid bias generated by uptake sEVs sperm cells, employing increasing concentrations hydrogen peroxide (H 2 O ) that generate single-strand breaks (SSBs). Because SSBs contain free 3’-OH end allow extension through quantitative PCR, such -and therefore SYBR intensity- showed proportional amount SSB. In next stage, H co-incubated with two size-differentiated subpopulations (small large) permeabilized non-permeabilized whether intravesicular content (IC) or surface protects from oxidative damage. obtained small reduced incidence ( P < 0.05), whereas large had no impact generation > 0.05). IC protective effect against Conclusions Our results suggest sEVs, including peripheral corona layer, exert function alterations are originated mechanisms. addition, our vitro study opens path detect, localize quantify derived different fluids, elucidating their physiopathological states.

Language: Английский

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