Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 26, 2025

Asthma is a prevalent respiratory disease, and its management remains largely unsatisfactory. Mesenchymal stem cells (MSCs) have been demonstrated to be efficacious in reducing airway inflammation experimental allergic diseases, representing potential alternative treatment for asthma. Migrasomes are recently identified extracellular vesicles (EVs) generated migrating facilitate intercellular communication. The objective of this study was investigate the therapeutic effects migrasomes obtained from MSC model produced by human umbilical cord MSCs (hUCMSCs) were isolated sequential centrifugation. Characterization hUCMSC-derived carried out transmission electron microscopy western blot analysis. on ovalbumin (OVA)-induced asthmatic mice evaluated hematoxylin-eosin (HE) periodic-acid schiff (PAS) staining, their mechanism further testified immunofluorescent real-time PCR flow cytometry. Here, we showed that inhibition migrasomes' production dramatically impaired anti-inflammatory hUCMSCs OVA animals, as evidenced notable increase both infiltration inflammatory number epithelial goblet cells. We successfully hUCMSC-migrasomes, which morphologically intact positive specific markers. administration hUCMSC-migrasomes observed significantly ameliorate symptoms mucus mice. Additionally, expression Th2 cytokines (IL-4, IL-5 IL-13) found reduced, while activation dendritic (DCs) inhibited. HUCMSC-migrasomes could possibly delivered lung region after injection, able taken DCs vivo vitro. Notably, vitro, migraosmes decreased capacity BMDCs stimulate OVA-specific Th2-cell responses. More importantly, adoptive transfer hUCMSC-migrasomes-treated sufficient protect inflammation. In addition, inhibited receptor advanced glycation end-products (RAGE) signal OVA-treated vitro asthma vivo. Our results provided first evidence possess properties OVA-induced mice, may provide novel "MSC-cell free" agent

Language: Английский

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Exploring the landscape of Lipid Nanoparticles (LNPs): A comprehensive review of LNPs types and biological sources of lipids

International Journal of Pharmaceutics X, Journal Year: 2024, Volume and Issue: 8, P. 100305 - 100305

Published: Nov. 18, 2024

Language: Английский

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Safety, Efficacy and Bio-Distribution Analysis of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells for Effective Treatment of Bronchopulmonary Dysplasia by Intranasal Administration in Mice Model

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 2521 - 2553

Published: Feb. 1, 2025

Exosomes (Exos) derived from human umbilical cord mesenchymal stem cells (hUC-MSCs) hold great potential for treating bronchopulmonary dysplasia (BPD); however, safety concerns and effects of intranasal administration remain unexplored. This study aimed to explore the hUC-MSCs Exos investigate efficacy bio-distribution repeated in neonatal BPD models. Characteristics were analyzed. A subcutaneous tumor formation assay using a single dose or was conducted Crl:NU-Foxn1nu mice. Vital signs, biochemical indices, pathological alterations, 18F-FDG microPET/CT analysis examined. Pulmonary pathology, three-dimensional reconstructions, ultrastructural structures, vivo ex imaging analyses, enzyme-linked immunoassay assays, reverse transcription-quantitative polymerase chain reaction analyses lung tissues all documented following administration. satisfied specifications. mice did not exhibit overt toxicity carcinogenicity after 60 days observation. Repeated effectively alleviated injuries, restored pulmonary ventilation reconstruction, recovered endothelial cell layer integrity analysis. steadily accumulated postnatal day 1 14. also interrupted epithelial-mesenchymal transition inflammation reactions As nanoscale, non-cellular therapy, an effective, noninvasive treatment BPD. approach free toxic, tumorigenic risks repaired alveolar damage while interrupting with

Language: Английский

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Induced Pluripotent Stem Cell-Derived Exosomes Promote Peripheral Nerve Regeneration in a Rat Sciatic Nerve Crush Injury Model: A Safety and Efficacy Study

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 529 - 529

Published: April 2, 2025

Peripheral nerve injury (PNI) remains a significant clinical challenge, often leading to long-term functional impairment. Despite advances in therapies, current repair strategies offer unsatisfactory outcomes. Exosomes derived from induced pluripotent stem cells (iPSC-Exos) have emerged as promising therapeutic approach regenerative medicine. This study assesses the efficacy and safety of iPSC-Exos rat model sciatic crush injury. Briefly, iPSCs were generated peripheral blood mononuclear (PBMCs) healthy donors using Sendai virus vectors validated for pluripotency. characterized injected at site. Functional recovery was assessed through gait analysis, grip strength, pain response. Histological molecular analyses used examine axonal regeneration, myelination, Schwann cell (SC) activation, angiogenesis, changes gene expression. efficiently internalized by SC, promoting their proliferation. No adverse effects observed between groups on body weight, organ histology, or hematological parameters. injection significantly enhanced muscle preservation, vascularization, with RNA sequencing revealing activation PI3K-AKT focal adhesion pathways. These findings support safe effective non-cell-based therapy PNIs, highlighting potential applications

Language: Английский

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Immunological Safety Evaluation of Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells in Mice

Stem Cells International, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Mounting evidence indicates that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs‐exosomes) combine the advantages of hucMSC pluripotency with their nanoscale dimensions, enhancing clinical potential through prolonged circulation half‐life. Despite these promising characteristics, research on immunological toxicity remains insufficient. This study focuses impact hucMSC‐exosomes general and immunopathological indicators. When mice received tail vein injections 6 × 10 particles, we observed no significant changes in body weight, feed intake, blood composition, organ indices, or histopathological findings throughout 14 days observation period. Similarly, levels immunoglobulins, cytokines, lymphocyte subpopulations remained stable. The produced detectable negative effects immune organs including thymus, spleen, bone marrow. These indicate intravenous administration particles appears relatively safe at murine level. assessment safety following infusion offers experimental support for applications future analyses this field.

Language: Английский

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Identification of chemical composition in Gnetum montanum extract and plasma components after oral administration in cynomolgus monkey by UPLC-Q-TOF-MS and its anti-tumor active components analysis

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2024, Volume and Issue: 249, P. 116347 - 116347

Published: July 9, 2024

Gnetum montanum Markgr. (Gnetaceae) is a commonly used traditional herbal medicine among the Yao ethnic group, with potential effects in preventing and treating tumors. However, substance basis of its anti-tumor properties remains unclear. This study utilized ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to identify chemical components G. extract (GME) absorbed prototypes cynomolgus monkey plasma after oral administration. A total 57 compounds were detected GME, 14 positive ion mode 43 negative mode. In plasma, 17 identified, 3 Subsequently, we high content screening technology investigate GME on colon cancer, lung breast gastric liver esophageal cancer. We found that exhibited significant proliferation inhibition cancer cells SW480, an IC

Language: Английский

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