Exploring the ncRNA landscape in exosomes: Insights into wound healing mechanisms and therapeutic applications DOI

A Gowtham,

Ravinder K. Kaundal

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 139206 - 139206

Published: Dec. 1, 2024

Language: Английский

Calcitriol Treated Mesenchymal Stem Cells Modulated Immune Response in Collagen-Induced Rheumatoid Arthritis in BALB/c Mice DOI

Alireza Rafati,

Reihaneh Ramezani, Hadi Esmaeili Gouvarchin Ghaleh

et al.

Transplantation Proceedings, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Inflammatory memory-activated biomimetic nanovesicles regulate neutrophil plasticity and metabolic reprogramming for rapid diabetic wound healing via targeting miR-193a-5p/TLR4/JNK/P38 MAPK pathways DOI Creative Commons
Yunlong Fan,

Jiaman Yang,

Yulin Xie

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 17, 2025

Language: Английский

Citations

0
Repeat-dose toxicity of human umbilical cord mesenchymal stem cells via subcutaneous injection in NOG mice DOI Creative Commons

Lijuan Xia,

Jinjin Shao,

Qian Yang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 3, 2025

Background Stem cell therapy shows promise for treating skin diseases and enhancing medical aesthetics. However, safety data subcutaneous injection of stem cells remain limited. In this study, we evaluated the toxicity human umbilical cord mesenchymal (hUC-MSCs) in NOD. Cg-Prkdc scid IL2rg tm1Sug /JicCrl (NOG) mice. Methods Mice received hUC-MSC injections at doses 2.5 × 10 7 2.0 8 cells/kg on days 1, 8, 12, 16, 20, followed by withdrawal observation 6 weeks. Toxicity was assessed through clinical observation, behavioral analysis, pathology, organ weight measurements, histopathology. distribution determined via validated quantitative (q)PCR colonization using immunohistochemistry. Results No abnormal effects responses, body weight, or food intake were observed following five repeated hUC-MSCs administrations, except masses administration site high-dose group. Mouse activity levels increased both dose groups h post-final injection. Foamy under pleural membrane primarily colonized distributed within tissues 24 after last administration. Conclusion The no-observed-adverse-effect level NOG mice over 3 weeks cells/kg. Our results will help advancing use hUC-MSCs, particularly conditions such as atopic dermatitis.

Language: Английский

Citations

0
Methacryloylated chitosan hydrogel-mediated polyphenol-Ga/hUCMSC-Exo release platform: Possessing antibacterial, anti-inflammatory, and osteogenic capabilities DOI
Hui Su,

Fengzhen Jia,

Zhi Hui Tian

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 309, P. 142893 - 142893

Published: April 9, 2025

Language: Английский

Citations

0
Therapeutic Efficacy and Promise of Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles in Aging and Age-Related Disorders DOI Open Access
Anyuan Zhang, Qiubai Li, Zhichao Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 225 - 225

Published: Dec. 30, 2024

The global issue of aging populations has become increasingly prominent, thus the research and development for anti-aging therapies to assure longevity as well ameliorate age-related complications is put high on agenda. young humoral milieu been substantiated impart youthful characteristics aged cells or organs. Extracellular vesicles (EVs) are a heterogeneous group cell-derived membrane-limited structures that serve couriers proteins genetic material regulate intercellular communication. Of note, EVs appeared be an indispensable component blood in prolonging lifespans, circulating have indicated mediate beneficial effect aging. Human umbilical cord mesenchymal stem (HUCMSC-EVs), isolated from youngest adult cell source, speculated reproduce function partially revitalize numerous organs old animals. Robust evidence suggested HUCMSC-EVs muti-target therapeutic agents combating alleviating degenerative disorders. Here, we provide comprehensive overview effects brain, heart, vasculature, kidney, muscle, bone, other Furthermore, critically discuss current investigation engineering strategies HUCMSC-EVs, intending unveil their full potential field research.

Language: Английский

Citations

2
Application of exosomes for the regeneration of skin wounds: Principles, recent applications and limitations DOI
Ali Esmaeili, Ghasem Noorkhajavi, Masoud Soleimani

et al.

Tissue and Cell, Journal Year: 2024, Volume and Issue: 91, P. 102611 - 102611

Published: Nov. 10, 2024

Language: Английский

Citations

0
Exploring the Molecular Mechanism of Estrogen Therapy Effectiveness after TCRA in IUA Patients at single-cell level DOI Creative Commons
Yue Du, Ruzhen Shuai, Sang Luo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Background Intrauterine adhesion (IUA) is a common cause of clinically refractory infertility, and there exists significant heterogeneity in the treatment outcomes among IUA patients with similar severity after transcervical resection adhesion(TCRA). The underlying mechanism different occur remains elusive, precise contribution various cell subtypes this process uncertain. Results Here, we performed single-cell transcriptome sequencing on 10 human endometrial samples to establish atlas differences between who responded estrogen therapy those did not. results showed increased infiltration immune cells such as monocyte macrophages, T cells, natural killer (NK) not respond therapy. Our findings indicate that distinct fibroblast subsets are implicated modulation Wnt, Hippo, Hedgehog signaling pathways, evidenced by functional enrichment analyses. This may have implications for therapeutic efficacy IUA. Furthermore, delineated markers transcriptional status macrophage identified two clusters, CXCL10high CCL4L2high subsets, which intimately associated inflammation fibrosis. state fibrosis inflammatory response tissues disparate revealed, providing evidence clarify determinants sensitivity Conclusions We described groups patients, new ideas exploring molecular difference effectiveness theoretical basis individualized plans patients.

Language: Английский

Citations

0
Exploring the molecular mechanism of estrogen therapy effectiveness after TCRA in IUA patients at single-cell level DOI Creative Commons
Yue Du, Ruzhen Shuai, Sang Luo

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 26, 2024

Intrauterine adhesion (IUA) is a common cause of clinically refractory infertility, and there exists significant heterogeneity in the treatment outcomes among IUA patients with similar severity after transcervical resection adhesion(TCRA). The underlying mechanism different occur remains elusive, precise contribution various cell subtypes this process uncertain. Here, we performed single-cell transcriptome sequencing on 10 human endometrial samples to establish atlas differences between who responded estrogen therapy those did not. results showed increased infiltration immune cells such as monocyte macrophages, T cells, natural killer (NK) not respond therapy. Our findings indicate that distinct fibroblast subsets are implicated modulation Wnt, Hippo, Hedgehog signaling pathways, evidenced by functional enrichment analyses. This may have implications for therapeutic efficacy IUA. Furthermore, delineated markers transcriptional status macrophage identified two clusters, CXCL10high CCL4L2high subsets, which intimately associated inflammation fibrosis. state fibrosis inflammatory response tissues disparate revealed, providing evidence clarify determinants sensitivity We described groups patients, new ideas exploring molecular difference effectiveness theoretical basis individualized plans patients.

Language: Английский

Citations

0
Exploring the ncRNA landscape in exosomes: Insights into wound healing mechanisms and therapeutic applications DOI

A Gowtham,

Ravinder K. Kaundal

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 139206 - 139206

Published: Dec. 1, 2024

Language: Английский

Citations

0