Abstract
Early
tumor
recurrence
in
hepatocellular
carcinoma
(HCC)
remains
a
challenging
area,
as
the
mechanisms
involved
are
not
fully
understood.
While
microvascular
invasion
is
linked
to
early
recurrence,
established
biomarkers
for
diagnosis
and
prognostication
lacking.
In
this
study,
our
objective
was
identify
DNA
methylation
sites
that
can
predict
outcomes
of
liver
cancer
patients
elucidate
molecular
driving
HCC
aggressiveness.
Using
methylome
data
from
patient
samples
CGRC
TCGA
databases,
we
pinpointed
hypermethylated
CpG
HCC.
Our
analysis
revealed
cg02746869
acts
crucial
regulatory
site
VIM-AS1
(vimentin
antisense
RNA1),
1.8
kb
long
noncoding
RNA.
RNA
sequencing
cells
with
manipulated
expression
EPHA3
pathogenic
target
,
which
performs
an
oncogenic
function
Hypermethylation-induced
suppression
significantly
impacted
cell
dynamics,
particularly
impairing
motility
invasiveness.
Mechanistically,
reduced
stabilized
mRNA
by
enhancing
binding
IGF2BP1
mRNA,
leading
increased
promotion
progression.
vivo
experiments
further
confirmed
VIM-AS1‒EPHA3
axis
controlled
growth
microenvironment
These
findings
suggest
downregulation
due
hypermethylation
at
via
m6A-dependent
mechanism
increase
PubMed,
Journal Year:
2024,
Volume and Issue:
44(1), P. 17 - 24
Published: Jan. 20, 2024
To
investigate
the
effect
of
overexpression
LncRNA
MEG3
on
proliferation,
migration
and
cisplatin
sensitivity
hepatoma
cells
HepG2
LM3
explore
underlying
mechanism.
Frontiers in Medicine,
Journal Year:
2024,
Volume and Issue:
11
Published: Sept. 11, 2024
Polycystic
ovary
syndrome
(PCOS)
can
lead
to
infertility
and
increase
the
risk
of
endometrial
cancer.
Analyzing
macrophage
polarization
characteristics
in
ovarian
tissues
PCOS
is
crucial
for
clinical
treatment.
Heliyon,
Journal Year:
2023,
Volume and Issue:
9(11), P. e22083 - e22083
Published: Nov. 1, 2023
The
increasing
evidence
suggests
that
necroptosis
mediates
many
behaviors
of
tumors,
as
well
the
regulation
tumor
microenvironment.
Long
non-coding
RNAs
(lncRNAs)
are
involved
in
a
variety
regulatory
processes
during
development
and
significantly
associated
with
patient
prognosis.
It
necroptosis-related
lncRNAs
(NRlncRNAs)
may
serve
biomarkers
for
prognosis
hepatocellular
carcinoma
(HCC).lncRNA
expression
profiles
HCC
were
obtained
from
TCGA
database.
LncRNAs
extracted
using
correlation
analysis.
Prognostic
models
constructed
based
on
least
absolute
shrinkage
selection
operator
algorithm
(LASSO)
multivariate
Cox
regression
differences
microenvironment
between
high-risk
low-risk
groups
further
analyzed.
Single-cell
RNA
sequencing
data
was
performed
to
assess
enrichment
genes
immune
cell
subsets.
Finally,
real-time
RT-PCR
used
detect
prognosis-related
different
lines.We
prognostic
signature
8
NRlncRNAs,
which
also
showed
good
predictive
accuracy.
model
patients
score
worse
than
(P
<
0.05).
Combined
clinical
characteristics
risk
HCC,
Nomogram
drawn
reference
practice.
In
addition,
infiltration
analysis
single
low
level
observed
at
high
there
significant
NRlncRNAs
macrophages.
results
RT-qPCR
highly
expressed
lines
human
liver
cancer
tissues.This
provide
meaningful
insights
immunotherapy
responses
HCC.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 5, 2024
Abstract
Background
Early
tumor
recurrence
observed
in
hepatocellular
carcinoma
(HCC)
remains
challenging,
as
the
mechanisms
involved
have
not
been
completely
elucidated.
Microvascular
invasion
is
associated
with
early
recurrence;
however,
well-established
biomarkers
for
diagnosis
and
predicting
prognosis
are
lacking.
In
this
study,
we
aimed
to
identify
DNA
methylation
sites
liver
cancer
patient
validate
HCC
aggressiveness
molecular
mechanisms.
Methods
methylome
data
from
samples
(CGRC
TCGA)
were
analyzed
hypermethylated
CpG
sites.
RNA-sequencing
was
performed
on
cells
modulated
expression
of
VIM-AS1
,
a
long
non-coding
RNA
regulated
by
methylation.
vitro
vivo
studies
investigated
intracellular
mechanism
action
CRISPR-dCas9
system
used
validating
potential
targeted
therapeutic
target.
Results
We
that
cg02746869,
significantly
prognosis,
acted
pivotal
regulatory
site
.
Suppression
due
profoundly
influenced
cellular
dynamics,
specifically
impairing
motility
invasiveness
cells.
This
effect
modulating
EPHA3
its
subsequent
interaction
m6A-associated
protein,
IGF2BP1.
Additionally,
modifications
state
cg02746869
directly
affected
invasive
properties
cells,
underscoring
critical
role
oncogenic
behavior
HCC.
Conclusions
Our
results
highlighted
significant
controlling
lncRNA
impact
pathophysiology.
Thus,
an
emergent
biomarker
prognostic
evaluation
intervention
BioData Mining,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: April 17, 2024
Abstract
Competing
endogenous
RNAs
play
key
roles
in
cellular
molecular
mechanisms
through
cross-talk
post-transcriptional
interactions.
Studies
on
ceRNA
cross-talk,
which
is
particularly
dependent
the
abundance
of
free
transcripts,
generally
involve
large-
and
small-scale
studies
involving
integration
transcriptomic
data
from
tissues
correlation
analyses.
This
abundance-dependent
nature
interactions
suggests
that
tissue-
condition-specific
dynamics
may
fluctuate.
However,
there
are
no
comprehensive
investigating
normal
tissue,
ceRNAs
lost
and/or
appear
cancerous
or
their
In
this
study,
we
comprehensively
analyzed
tumor-specific
fluctuations
observed
three
highest-incidence
cancers,
LUAD,
PRAD,
BRCA,
compared
to
healthy
lung,
prostate,
breast
tissues,
respectively.
Our
observations
pertaining
competing
RNA
(ceRNA)
revealed
that,
cases
lung
adenocarcinoma
(LUAD),
prostate
(PRAD),
invasive
carcinoma
(BRCA),
3,204,
1,233,
406
ceRNAs,
respectively,
engage
intercommunication
within
tumor
contrast
absence
corresponding
samples.
We
also
found
90
shared
by
cancer
types
these
participate
those
tissues.
Among
directly
interact
with
miRNAs,
uncovered
a
core
network
165
miRNAs
63
should
be
considered
RNA-targeted
RNA-mediated
approaches
future
could
used
aggressive
types.
More
specifically,
interaction
network,
such
as
GALNT7,
KLF9,
DAB2
like
miR-106a/b-5p,
miR-20a-5p,
miR-519d-3p
have
potential
common
targets
critical
cancers.
conventional
methods
construct
networks
using
differentially
expressed
genes
our
proposed
approach
identifies
players
considering
context
ceRNA:miRNA
results
reveal
distinct
pinpoint
RNAs,
thereby
paving
way
for
RNA-based
strategies
battle
against
cancer.
Current Pharmaceutical Design,
Journal Year:
2024,
Volume and Issue:
30(27), P. 2120 - 2128
Published: June 10, 2024
:
Primary
liver
cancer
is
the
second
leading
cause
of
cancer-related
death
worldwide.
At
present,
often
in
an
advanced
stage
once
diagnosed,
and
treatment
effects
are
generally
poor.
Therefore,
there
urgent
need
for
other
powerful
treatments.
Macrophages
important
component
tumor
microenvironment,
macrophage
polarization
crucial
to
proliferation
differentiation.
Regulatory
interactions
between
subtypes,
such
as
M1
M2,
lead
a
number
clinical
outcomes,
including
progression
metastasis.
So,
it
study
drivers
this
process.
Long
non-coding
RNA
has
been
widely
proven
be
great
value
early
diagnosis
tumors.
Many
studies
have
shown
that
long
participates
through
its
ability
drive
or
M2
polarization,
thereby
participating
occurrence
development
cancer.
In
article,
we
systematically
elaborated
on
RNAs
involved
macrophages,
hoping
provide
new
idea
Liver
cancer-
related
were
retrieved
from
PubMed.
Based
our
identification
lncRNA
therapies
cancer,
analyzed
research
articles
PubMed
system
last
ten
years
crosstalk
polarization.
By
targeting
M1/M2
may
promote
suppress
references
determined
primarily
by
article's
impact
factor.
Consequently,
specific
mechanism
action
was
explored,
along
with
role
their
occurrence,
proliferation,
metastasis
LncRNA
bidirectionally
expressed
can
target
regulate
behavior.
mainly
functions
ceRNA
participate
cells
macrophages
extracellular
vesicles.
potentially
immunotherapy
becoming
biomolecular
marker
World Journal of Gastrointestinal Oncology,
Journal Year:
2024,
Volume and Issue:
16(7), P. 3082 - 3096
Published: July 11, 2024
Hepatocellular
carcinoma
(HCC)
is
a
malignant
tumor
that
has
high
incidence
and
mortality
worldwide.
Despite
extensive
studies,
the
detailed
molecular
mechanism
of
HCC
development
remains
unclear.
Studies
have
shown
occurrence
are
closely
related
to
abnormal
gene
expression.
Abstract
Early
tumor
recurrence
in
hepatocellular
carcinoma
(HCC)
remains
a
challenging
area,
as
the
mechanisms
involved
are
not
fully
understood.
While
microvascular
invasion
is
linked
to
early
recurrence,
established
biomarkers
for
diagnosis
and
prognostication
lacking.
In
this
study,
our
objective
was
identify
DNA
methylation
sites
that
can
predict
outcomes
of
liver
cancer
patients
elucidate
molecular
driving
HCC
aggressiveness.
Using
methylome
data
from
patient
samples
CGRC
TCGA
databases,
we
pinpointed
hypermethylated
CpG
HCC.
Our
analysis
revealed
cg02746869
acts
crucial
regulatory
site
VIM-AS1
(vimentin
antisense
RNA1),
1.8
kb
long
noncoding
RNA.
RNA
sequencing
cells
with
manipulated
expression
EPHA3
pathogenic
target
,
which
performs
an
oncogenic
function
Hypermethylation-induced
suppression
significantly
impacted
cell
dynamics,
particularly
impairing
motility
invasiveness.
Mechanistically,
reduced
stabilized
mRNA
by
enhancing
binding
IGF2BP1
mRNA,
leading
increased
promotion
progression.
vivo
experiments
further
confirmed
VIM-AS1‒EPHA3
axis
controlled
growth
microenvironment
These
findings
suggest
downregulation
due
hypermethylation
at
via
m6A-dependent
mechanism
increase
