Unveiling the potency of ZnO and CuO nanocomposites in combating hepatocellular carcinoma by inducing cell death and suppressing migration DOI Creative Commons
Rasha Mahmoud Allam,

Nesma M. E. Abo El-Nasr,

Marawan A. Elbaset

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 3, 2025

Abstract Human hepatocellular carcinoma (HCC) is recognized as one of the leading causes death globally and resistant to several anticancer drugs. As a result, it critical identify more effective druggable therapies. Metal oxide nanoparticles (MO-NPs), especially nanocomposites, have recently received much attention owing their potential applications in cancer therapy. In this study, we synthesized zinc (ZnO) copper (CuO) nanocomposites different ratios (N1, N2, N3). We evaluated cytotoxicity against two HCC cell lines (HepG2 HuH-7) normal liver (BNL), compared with Sorafenib standard Then, investigated underlying mechanisms action employing flow cytometry, migration assay, western blot. The results showed that nanocomposite an equal ratio both ZnO CuO-NPs (N1) exhibited highest cytotoxic activity on HuH7 line while exerting no detrimental impact rat epithelial cells. Further investigation into toxicity N1 revealed three modalities induced (apoptotic, necrotic, autophagic) along S- G2/M cycle arrest, suggesting mitotic catastrophe. Furthermore, displayed potent anti-migratory activity, surpassing sorafenib, upregulated protein level autophagy marker beclin-1, downregulated EMT-marker vimentin. Overall, our findings combining ZnO-NPs intriguing combating HCC, providing prospective guidance for evolving therapy bimetallic NPs.

Language: Английский

The Dual Role of LncRNAs in Hepatocellular Carcinoma: Friend and Foe DOI Creative Commons

Farzaneh Abam,

‪Saeid Ghorbian

Gastroenterology & Endoscopy, Journal Year: 2024, Volume and Issue: 2(4), P. 186 - 195

Published: June 4, 2024

In eukaryotes, RNA molecules called long non-coding RNAs (LncRNAs) have been found. Molecules that exceed 200 base pairs in length are incapable of coding for proteins. Studying the function LncRNAs cancer has main focus current investigation, which significantly impacted protein integrity and stability. can be regulated through various pathways, leading to chromatin changes methylation or as miRNA sponges. The most common type liver tumor worldwide is hepatocellular carcinoma (HCC), accounting around 80% subjects. Nonetheless, insufficiency trustworthy molecular markers remains a significant challenge HCC diagnosis treatment evaluation. HCC, such MEG3, MALAT1, HULC, HOTAIR, H19 dysregulation closely associated with expansion, metastasis, prognosis, diagnosis. primary goal this investigation was investigate newly discovered roles mechanisms pathophysiology cells identify potential therapeutic targets.

Language: Английский

Citations

4
CCDC154 knockdown inhibits growth of liver cancer via suppressing expression of Snail DOI Creative Commons

Rulan Ma,

Hongmei Nie,

Caijing Mo

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: Jan. 30, 2025

The effect of coiled-coil domain-containing 154 (CCDC154) in liver cancer (LC) remains unexplored. objective this study was to investigate the role CCDC154 LC and its underlying mechanism. analysis expression prognosis performed using UALCAN, Human Protein Atlas Kaplan–Meier plotter websites. measured Western blotting assay. Lentivirus used silence cells. proliferation apoptosis cells evaluated by cell counting assay, colony formation assay flow cytometry. migration invasion were investigated scratch wound-healing Transwell results showed that highly expressed related tumor grade stage. High associated with poor outcomes patients. Silencing inhibited proliferation, It also increased After knockdown, Twist, Vimentin Snail down-regulated. Overexpression abated inhibitory caused knockdown on growth. suppressed development through reducing expression.

Language: Английский

Citations

0
MICAL1 Mediates TGF-β1-Induced Epithelial-to-Mesenchymal Transition and Metastasis of Hepatocellular Carcinoma by Activating Smad2/3 DOI
Xun Zhuang,

Chunrong Wang,

Zhenghui Ge

et al.

Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Language: Английский

Citations

0
Unveiling the potency of ZnO and CuO nanocomposites in combating hepatocellular carcinoma by inducing cell death and suppressing migration DOI Creative Commons
Rasha Mahmoud Allam,

Nesma M. E. Abo El-Nasr,

Marawan A. Elbaset

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 3, 2025

Abstract Human hepatocellular carcinoma (HCC) is recognized as one of the leading causes death globally and resistant to several anticancer drugs. As a result, it critical identify more effective druggable therapies. Metal oxide nanoparticles (MO-NPs), especially nanocomposites, have recently received much attention owing their potential applications in cancer therapy. In this study, we synthesized zinc (ZnO) copper (CuO) nanocomposites different ratios (N1, N2, N3). We evaluated cytotoxicity against two HCC cell lines (HepG2 HuH-7) normal liver (BNL), compared with Sorafenib standard Then, investigated underlying mechanisms action employing flow cytometry, migration assay, western blot. The results showed that nanocomposite an equal ratio both ZnO CuO-NPs (N1) exhibited highest cytotoxic activity on HuH7 line while exerting no detrimental impact rat epithelial cells. Further investigation into toxicity N1 revealed three modalities induced (apoptotic, necrotic, autophagic) along S- G2/M cycle arrest, suggesting mitotic catastrophe. Furthermore, displayed potent anti-migratory activity, surpassing sorafenib, upregulated protein level autophagy marker beclin-1, downregulated EMT-marker vimentin. Overall, our findings combining ZnO-NPs intriguing combating HCC, providing prospective guidance for evolving therapy bimetallic NPs.

Language: Английский

Citations

0