MicroRNA-142-3p Overcomes Drug Resistance in Hepatocellular Carcinoma by Targeting YES1 and TWF1

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4161 - 4161

Published: April 27, 2025

Resistance to tyrosine kinase inhibitors (TKIs, e.g., sorafenib and lenvatinib) presents a significant hurdle for hepatocellular carcinoma (HCC) treatment, underscoring the need decipher underlying mechanisms improved therapeutic strategies. MicroRNAs (miRNAs) have emerged as critical modulators in HCC progression TKI resistance. In this study, we report positive correlation between expression levels of tumor suppressor miRNA, miR-142-3p, increased sensitivity lenvatinib, supported by clinical data from BIOSTORM cohort. Overexpression miR-142-3p TKI-resistant cells significantly inhibited proliferation colony formation, induced apoptosis, cell cycle arrest at G2 phase, reduced migration invasion reversing epithelial–mesenchymal transition. Notably, combining with lenvatinib synergistically growth both inherent acquired modulating signaling pathways, including STAT3, PI3K/AKT, MAPK, YAP1, impeding autophagic influx. RNA-sequencing line ± overexpression identified YES1 TWF1 direct downstream target genes which are key associated drug resistance HCC. Small interfering RNA (siRNA)-mediated knockdown these mirrored antitumor effects enhanced sensitivity, decreasing YAP1 phosphorylation, inhibiting autophagy. Collectively, findings indicate that restoring or targeting its effectors offers promising strategy overcome improve outcome

Language: Английский

Global research status and frontiers on ferroptosis in hepatocellular carcinoma: a comprehensive bibliometric and visualized analysis

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 2, 2025

Objective The purpose of this study was to analyze the research hotspots and future trends in field ferroptosis hepatocellular carcinoma past 10 years by using bibliometrics visualization software, provide reference for directions field. Methods Web Science database searched from January 1, 2012, October 30, 2024, annual publication volume, countries, institutions, journals, authors, references, keywords, other information were analyzed bibliometrics, VOSviewer, CiteSpace. Results A total 645 English articles 729 institutions 32 countries included study, with 4545 authors published 261 journals. In three years, 518 published, accounting 80.3%. China has most publications, followed United States. Frontiers Oncology had highest number papers (n=26), while Cell citations (n=1206). current mainly focuses on two aspects: one is mechanism explore new therapeutic targets, exploration methods, such as photodynamic therapy nanomaterials, order inhibit proliferation tumor cells, reduce drug resistance, enhance efficacy regulating ferroptosis, which may become a development trend. Conclusion recent there have been increasing studies association between carcinoma. This first comprehensive bibliometric provides reliable promotes its further development.

Language: Английский

Brusatol improves the efficacy of sorafenib in Huh7 cells via ferroptosis resistance dependent Nrf2 signaling pathway

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 734, P. 150762 - 150762

Published: Sept. 27, 2024

Language: Английский