Sitagliptin regulates the AMPK/NF-κB signaling pathway to alleviate lipopolysaccharide-induced inflammatory responses and promote osteogenic differentiation in rat bone marrow mesenchymal stem cells DOI Creative Commons
Xi Yao, Min Liu, Pei Wang

et al.

Archives of Oral Biology, Journal Year: 2025, Volume and Issue: unknown, P. 106253 - 106253

Published: April 1, 2025

This study aimed to explore the impact of sitagliptin on inflammatory response and osteogenic differentiation in lipopolysaccharide (LPS)-stimulated rat bone marrow mesenchymal stem cells (BMSCs) clarify underlying mechanisms action. In vitro-cultured BMSCs were identified, treated with a range doses, assessed cell counting kit-8 assay quantify viability. The expression proteins genes relevant inflammation osteogenesis was measured using enzyme-linked immunosorbent real-time quantitative polymerase chain reaction techniques. ability analyzed by alkaline phosphatase staining, activity assay, alizarin red s staining. Adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-kappa B (NF-κB) signaling pathway activation detected through western blotting. High but not low concentrations significantly suppressed cellular Sitagliptin dose-dependently inhibited LPS-induced responses while facilitating their differentiation. It also activated AMPK suppressing NF-κB activity. inhibitor treatment partially reversed these beneficial effects osteogenesis. suppresses promoting modulation AMPK/NF-κB activity, thereby mitigating functional impairment under microenvironmental conditions.

Language: Английский

Sitagliptin regulates the AMPK/NF-κB signaling pathway to alleviate lipopolysaccharide-induced inflammatory responses and promote osteogenic differentiation in rat bone marrow mesenchymal stem cells DOI Creative Commons
Xi Yao, Min Liu, Pei Wang

et al.

Archives of Oral Biology, Journal Year: 2025, Volume and Issue: unknown, P. 106253 - 106253

Published: April 1, 2025

This study aimed to explore the impact of sitagliptin on inflammatory response and osteogenic differentiation in lipopolysaccharide (LPS)-stimulated rat bone marrow mesenchymal stem cells (BMSCs) clarify underlying mechanisms action. In vitro-cultured BMSCs were identified, treated with a range doses, assessed cell counting kit-8 assay quantify viability. The expression proteins genes relevant inflammation osteogenesis was measured using enzyme-linked immunosorbent real-time quantitative polymerase chain reaction techniques. ability analyzed by alkaline phosphatase staining, activity assay, alizarin red s staining. Adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-kappa B (NF-κB) signaling pathway activation detected through western blotting. High but not low concentrations significantly suppressed cellular Sitagliptin dose-dependently inhibited LPS-induced responses while facilitating their differentiation. It also activated AMPK suppressing NF-κB activity. inhibitor treatment partially reversed these beneficial effects osteogenesis. suppresses promoting modulation AMPK/NF-κB activity, thereby mitigating functional impairment under microenvironmental conditions.

Language: Английский

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