Novel Clinical Insights into the Pathogenesis of Posttraumatic Elbow Stiffness: An Expression Profile Analysis of Contracted Joint Capsule in Human

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 167 - 182

Published: Jan. 1, 2025

Posttraumatic elbow stiffness is a complex complication with two characteristics of capsular contracture and heterotopic ossification. Currently, genomic mechanisms pathogenesis posttraumatic remain inadequately understood. This study aims to identify differentially expressed genes (DEGs) elucidate molecular networks stiffness, providing novel insights into disease at transcriptome level. Global sequencing was conducted on six samples from individuals three control fractures. Differentially (DEGs), microRNAs, long non-coding RNAs (LncRNAs) were identified analyzed. Functional enrichment analysis performed, the associated protein-protein interaction (PPI) network constructed. MicroRNAs targeting these DEGs identified, transcription factors (TFs) predicted using ENCODE database. Finally, key validated by quantitative real-time polymerase chain reaction (qRT-PCR). A total 4909 protein-coding, LncRNA microRNA detected, including 2124 upregulated 2785 downregulated. Kyoto Encyclopedia Genes Genomes (KEGG) pathway revealed that significantly enriched in 36 signaling pathways, notably involving inflammatory responses extracellular matrix (ECM) receptor interactions. The highlighted such as SPP1, IBSP, MMP13 MYO1A having higher degrees connectivity. Key microRNAs (hsa-miR-186-5p, hsa-miR-515-5p, hsa-miR-590-3p) (TFDP1 STAT3) be implicated through microRNA-transcription factor regulatory analysis. provided underlying changes contracted capsules stiffness. Hub MMP13, MYO1A, (has-miR-186-5p, has-miR-515-5p, TFs may serve prognostic therapeutic targets provide new idea for future research direction clinical treatment.

Language: Английский

Metrnl plays a crucial role in the regulation of glycolipid metabolism

Metabolism and Target Organ Damage, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 25, 2025

An imbalance in the metabolism of glycolipids largely causes numerous metabolic diseases that negatively affect human health. Metrnl plays an important role glycolipid and regulates glucose lipid through multiple mechanisms. However, expression level related to remains controversial. can increase utilization by tissue cells reduce adverse effects a high-glucose environment on body; metabolism, it composition blood lipids, accumulation fat, promote browning white fat. The clinical efficacy exogenously administered recombinant needs be further investigated verified. This review presents overview Metrnl, emphasizing its association with diseases. It offers theoretical foundation for treatment metabolism-related conditions.

Language: Английский

METRNL mitigates oxidative stress and inflammatory drawbacks in ovalbumin/lipopolysaccharide-induced allergic airway diseases via the IKK/IκB/NF-κB signaling pathway

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract This study aimed to examine the potential impacts of METRNL as an antioxidant and anti-inflammatory through IκB kinase/inhibitor nuclear factor-kappa B/nuclear factor-kappa-light-chain signaling pathway on many biomarkers lung structure in rats with bronchial asthma induced by ovalbumin/lipopolysaccharide (OVA/LPS). Forty were randomly divided into four equal groups: control group, vehicle diseased (OVA/LPS) group OVA 2.5 ml/kg intratracheal installation/LPS 1.5 mg/kg intraperitoneally, treated (OVA/LPS + METRNL) at a dose 2 mg/rat/day IV. After 4 weeks, plasma tissues analyzed assess oxidative stress inflammatory markers. Additionally, histological assessment was conducted tissues. Bronchial confirmed when increased levels total serum IgE, cell count, neutrophils, eosinophils, macrophages, lymphocyte counts BAL fluid observed. Moreover, OVA/LPS resulted reduction superoxide dismutase (SOD) while raising malondialdehyde (MDA). Furthermore, it elevated concentrations mediators, including tumor necrosis factor-alpha (TNF-alpha), interleukin 17 (IL-17), transforming growth factor beta (TGF-β). The protective effects analyzed. observed are believed result from drug’s properties its action IKK/IκB/NF-κB pathway. investigation indicates that treatment positively improved rats’ biochemical aspects OVA/LPS-induced airway allergic inflammation.

Language: Английский