Effects of Acrylamide on Mouse Implantation and Decidualization

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4129 - 4129

Published: April 26, 2025

Acrylamide is a class 2A carcinogen with neurotoxicity and genotoxicity. In addition to industrial production, it ubiquitous in high-temperature heated high-carbohydrate foods. Numerous studies have confirmed the toxicity of ACR on reproduction. Implantation decidualization are crucial processes during establishment pregnancy rodents humans. However, its effect uterine implantation remains poorly understood. The objective this study elucidate mechanism by which affects mice. exposed daily drinking water female mice, dose calculated according body weight After 3 months administration at concentrations 0, 20, 30 mg ACR/kg/d, mice mated male induce pregnancy. Compared control group, treatment significantly reduces number embryo implantations litter size. leads abnormal expression endometrial receptivity-related molecules luminal epithelium day 4 pregnancy, including decrease p-STAT3 level an increase MUC1 MSX1 levels. decidualization-related obviously downregulated ACR. Furthermore, results abnormality oxidative stress- ferroptosis-related protein levels site 5. conclusion, acrylamide can impair mouse disrupting stress ferroptosis.

Language: Английский

Expression and purification of recombinant glutaredoxin 1 and protection against oxidative stress injury during cerebral ischemia-reperfusion injury

Protein Expression and Purification, Journal Year: 2025, Volume and Issue: unknown, P. 106689 - 106689

Published: Feb. 1, 2025

Language: Английский

Tomatidine Alleviates Intervertebral Disc Degeneration by Activating the Nrf2/HO-1/GPX4 Signaling Pathway

Drug Design Development and Therapy, Journal Year: 2024, Volume and Issue: Volume 18, P. 6313 - 6329

Published: Dec. 1, 2024

Intervertebral disc degeneration (IDD) is a leading cause of low back pain, and developing new molecular drugs targets for IDD direction future treatment strategies. The aim this study to investigate the effects mechanisms tomatidine in ameliorating lumbar IDD. Nucleus pulposus cells (NPCs) exposed lipopolysaccharides were used as an vitro model changes expression extracellular matrix components associated signaling pathway molecules. A instability was simulate Tomatidine (Td) then administered intraperitoneally, its evaluated through histopathological analysis. In vitro, Td significantly promoted ECM anabolism, inhibited catabolism, reduced oxidative stress ferroptosis LPS-stimulated NPCs. When Nrf2 inhibited, exacerbated, protective on NPCs lost, suggesting Nrf2/HO-1/GPX4 axis critical therapeutic Td. vivo, analysis demonstrated that ameliorated murine model. alleviates vivo by activating inhibit This mechanism suggests promising candidate treatment.

Language: Английский