Single-Cell RNA Sequencing Revealed Functional Conjunctival Keratinocytes Loss via TGF-β-Wnt/β-Catenin Signaling in Sjögren’s Syndrome Related Dry Eye DOI Creative Commons
Bowen Wang, Lihong Liang, Hao Zeng

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(4), P. 43 - 43

Published: April 16, 2025

The role of the conjunctiva in pathophysiology Sjögren's syndrome (SS) related dry eye disease (DED) remains obscure especially view functional conjunctival epithelia. In order to illustrate effects SS, we investigated interactions between parenchymal cells and immune with single-cell RNA sequencing technique. Freshly collected from a canonical SS model was prepared for 10 × Genomics T cell receptor (TCR) sequencing. Conjunctiva Western blot, immunofluorescence, multiplex immunohistochemical (mIHC), flow cytometry. Phenol red thread test, lissamine staining, qRT-PCR were applied evaluate signs DED. DED phenotype validated model. Loss water-secreting keratinocyte projected scRNA-seq data proved by mIHC test mice. proportion Lgr4+ basal epithelial poor ability differentiate into mature increased, Wnt/β-catenin signaling upregulated it under regulation TGF-β derived macrophages. Such macrophages promoted angiogenesis through secretion VEGFA activate endothelial cells. Immuno-fibroblasts had an increased population, which implicated specifically activated chemotaxis. conjunctiva, TGF-β-Wnt/β-catenin axis downregulated formation keratinocytes accompanied infiltration pro-angiogenetic pro-fibrotic macrophage pro-inflammatory cell.

Language: Английский

Single-Cell RNA Sequencing Revealed Functional Conjunctival Keratinocytes Loss via TGF-β-Wnt/β-Catenin Signaling in Sjögren’s Syndrome Related Dry Eye DOI Creative Commons
Bowen Wang, Lihong Liang, Hao Zeng

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(4), P. 43 - 43

Published: April 16, 2025

The role of the conjunctiva in pathophysiology Sjögren's syndrome (SS) related dry eye disease (DED) remains obscure especially view functional conjunctival epithelia. In order to illustrate effects SS, we investigated interactions between parenchymal cells and immune with single-cell RNA sequencing technique. Freshly collected from a canonical SS model was prepared for 10 × Genomics T cell receptor (TCR) sequencing. Conjunctiva Western blot, immunofluorescence, multiplex immunohistochemical (mIHC), flow cytometry. Phenol red thread test, lissamine staining, qRT-PCR were applied evaluate signs DED. DED phenotype validated model. Loss water-secreting keratinocyte projected scRNA-seq data proved by mIHC test mice. proportion Lgr4+ basal epithelial poor ability differentiate into mature increased, Wnt/β-catenin signaling upregulated it under regulation TGF-β derived macrophages. Such macrophages promoted angiogenesis through secretion VEGFA activate endothelial cells. Immuno-fibroblasts had an increased population, which implicated specifically activated chemotaxis. conjunctiva, TGF-β-Wnt/β-catenin axis downregulated formation keratinocytes accompanied infiltration pro-angiogenetic pro-fibrotic macrophage pro-inflammatory cell.

Language: Английский

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