Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Sept. 23, 2024
Introduction
Chagas
disease
is
a
neglected
tropical
caused
by
the
parasite
Trypanosoma
cruzi
that
transmitted
mainly
feces
of
infected
Triatomines.
In
Ecuador
main
vector
Rhodnius
ecuadoriensis
which
distributed
in
several
provinces
country.
More
than
40%
these
insects
wild
have
T.
as
part
their
intestinal
microbiota.
For
this
reason,
objective
research
was
to
characterize
bacterial
microbiota
R.
.
Methods
The
methodology
used
based
on
DNA
extraction
contents
from
collected
(adults
and
nymphs
V),
well
maintained
at
insectary
CISeAL.
Finally,
samples
were
analyzed
metagenomics
extensions
different
selected
criteria.
Results
presented
marked
divergence
between
laboratory-raised
insects.
This
difference
observed
all
stages
similar
Loja
Manabí.
A
large
loss
microbial
symbionts
Discussion
study
crucial
first
step
investigating
interactions
advancing
new
methodologies.
Journal of Chemical Information and Modeling,
Journal Year:
2023,
Volume and Issue:
64(7), P. 2565 - 2576
Published: Dec. 27, 2023
American
Trypanosomiasis,
also
known
as
Chagas
disease,
is
caused
by
the
protozoan
Trypanosoma
cruzi
and
exhibits
limited
options
for
treatment.
Natural
products
offer
various
structurally
complex
metabolites
with
biological
activities,
including
those
anti-T.
potential.
The
discovery
development
of
prototypes
based
on
natural
frequently
display
multiple
phases
that
could
be
facilitated
machine
learning
techniques
to
provide
a
fast
efficient
method
selecting
new
hit
candidates.
Using
Random
Forest
k-Nearest
Neighbors,
two
models
were
constructed
predict
activity
from
plants
against
intracellular
amastigotes
T.
cruzi.
diterpenoid
andrographolide
was
identified
virtual
screening
promising
compound.
Hereafter,
it
isolated
Cymbopogon
schoenanthus
chemically
characterized
spectral
data
analysis.
Andrographolide
evaluated
trypomastigote
amastigote
forms
cruzi,
showing
IC50
values
29.4
2.9
μM,
respectively,
while
standard
drug
benznidazole
displayed
17.7
5.0
respectively.
Additionally,
compound
exhibited
reduced
cytotoxicity
(CC50
=
92.8
μM)
mammalian
cells
afforded
selectivity
index
(SI)
32,
similar
(SI
39).
From
in
silico
analyses,
we
can
conclude
fulfills
many
requirements
implemented
DNDi
Therefore,
this
work
successfully
obtained
capable
predicting
compounds
Frontiers in Pharmacology,
Journal Year:
2022,
Volume and Issue:
13
Published: April 26, 2022
Background:
The
incidence
of
Nonalcoholic
Fatty
Liver
(NAFL)
is
increasing
year
by
year,
growing
evidence
suggests
that
the
intestinal
flora
plays
a
causative
role
in
NAFL.
Huazhi
Rougan
Granule
(HRG)
commonly
used
clinical
treatment
It
reported
it
can
reduce
lipids
and
protect
liver,
but
no
research
has
confirmed
whether
drug's
effect
related
to
flora.
Therefore,
we
investigated
HRG
regulation
further
explore
mechanism
NAFL
through
Methods:
In
this
study,
C57BL/6J
mice
were
fed
high-fat
diet
for
8
weeks,
plus
or
polyene
phosphatidylcholine
capsules
each
administered
gavage
4
weeks.
High-throughput
sequencing,
network
pharmacology,
molecular
docking
Results:
body
weight
gain,
lipid
accumulation
liver
lipogenesis
serum
biochemical
indexes
high-fat-fed
mice.
Analysis
showed
changed
composition
flora,
which
was
characterized
decrease
Firmicutes/Bacteroidetes
ratio.
Moreover,
species
distribution
significantly
correlated
with
AKP,
HDL-C,
TG.
Metagenetic
analysis
altered
functional
diversity
microorganisms,
mainly
an
increase
abundance
metabolic
pathways.
pharmacology
results
show
reflected
biological
process
gene
function
infectious
diseases,
immune
systems,
signal
transduction
pathways,
such
as
cytokine-cytokine
receptor
interaction,
Chagas
disease,
IL-17
signaling
pathway
other
Conclusion:
These
strongly
suggest
may
alleviate
preventing
IFD.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Sept. 23, 2024
Introduction
Chagas
disease
is
a
neglected
tropical
caused
by
the
parasite
Trypanosoma
cruzi
that
transmitted
mainly
feces
of
infected
Triatomines.
In
Ecuador
main
vector
Rhodnius
ecuadoriensis
which
distributed
in
several
provinces
country.
More
than
40%
these
insects
wild
have
T.
as
part
their
intestinal
microbiota.
For
this
reason,
objective
research
was
to
characterize
bacterial
microbiota
R.
.
Methods
The
methodology
used
based
on
DNA
extraction
contents
from
collected
(adults
and
nymphs
V),
well
maintained
at
insectary
CISeAL.
Finally,
samples
were
analyzed
metagenomics
extensions
different
selected
criteria.
Results
presented
marked
divergence
between
laboratory-raised
insects.
This
difference
observed
all
stages
similar
Loja
Manabí.
A
large
loss
microbial
symbionts
Discussion
study
crucial
first
step
investigating
interactions
advancing
new
methodologies.
