Pooled Antibiotic Susceptibility Testing for Polymicrobial UTI Performs Within CLSI Validation Standards

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 143 - 143

Published: Feb. 1, 2025

Background/Objectives: Urinary tract infections (UTIs) pose an increasing risk of antimicrobial resistance, and novel diagnostic tests have been developed to address the limitations standard urine culture in these cases. It is important that be validated for agreement error rates against antibiotic susceptibility testing (AST) methods. Methods: Polymicrobial (≥two non-fastidious microorganisms) consecutive clinical specimens submitted UTI were included this analysis. Specimens tested with Pooled Antibiotic Susceptibility Testing (P-AST) broth microdilution/disk diffusion (BMD/DD) parallel. Performance characteristics, such as essential (EA%), very major errors (VMEs), (MEs), assessed using Clinical Laboratory Standards Institute (CLSI) standards. P-AST-resistant BMD/DD consensus-sensitive results heteroresistance. Real-world sample data used assess associations between organism counts average “sensitive” count per sample. Results: The P-AST isolate AST was ≥90%, VMEs <2.0%, MEs <3.0%, meeting CLSI guidelines verification validation studies. When heteroresistance accounted for, overall both <1.5%. presence additional organisms dropped number antibiotics from 9.8 one 2.5 five or more organisms. fastidious did not any meaningful impact. Conclusions: P-AST, a component Guidance® assay (Pathnostics, Irvine, CA, USA), performed within standards polymicrobial specimens.

Language: Английский

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Microbiologist in the Clinic: Antibiotic Dependent in her 30 s

International Urogynecology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Language: Английский

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Interconnected microbiomes—insights and innovations in female urogenital health

FEBS Journal, Journal Year: 2024, Volume and Issue: unknown

Published: July 30, 2024

The paradigm that the vaginal microbiota is a protective gateway for urinary and reproductive systems has endured more than century driven decades of probiotic research. Evidence robustly supports notion healthy urogenital microbiomes are predominantly colonized by lactobacilli, particularly Lactobacillus crispatus , which can acidify local environment protect against urogynecologic pathogen colonization. However, recent studies beginning to delve deeper into intricate mechanistic interactions connecting microbiome, its diverse functional potential, host immunity, pathogens, development diseases. Leveraging these emerging insights alongside past successes presents promising opportunities future therapies aimed at enhancing management conditions such as bacterial vaginosis, tract infections, bladder pain, incontinence, beyond.

Language: Английский

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From Awareness to Action: Pioneering Solutions for Women’s UTI Challenges in the Era of Precision Medicine

International Journal of Women s Health, Journal Year: 2024, Volume and Issue: Volume 16, P. 1595 - 1605

Published: Sept. 1, 2024

This article aims to bring clinicians' awareness the widespread impact of urinary tract infection (UTI) on lives women and advances that offer hope for future improvements in diagnosis management UTI. Thanks physiological, anatomical, lifestyle factor differences, face heightened vulnerability UTIs compared men. In fact, are four times more likely than men develop a UTI around half these encounter recurrence, which is significant source both physical psychosocial burdens. Despite current shortcomings management, emerging diagnostic technologies promise identify accurately rapidly, offering revolution management. Meanwhile, clinicians have opportunity reduce burden by recognizing value patients' lived experiences ensuring their care plan alignment with goals expectations medical care.

Language: Английский

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Rapid and accurate testing for urinary tract infection: new clothes for the emperor

Clinical Microbiology Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

SUMMARY Urinary tract infection (UTI) is among the most common infections in clinical practice. In some cases, if left untreated, it can lead to pyelonephritis and urosepsis. other UTI resolves without treatment. Clinical diagnosis typically based on patient symptoms and/or urinalysis, including urine dipsticks. The standard culture method sometimes employed identify suspected urinary pathogen (uropathogen) guide antimicrobial choice, but results are rarely available before 24 h. also misses fastidious, anaerobic, slow-growing uropathogens reports polymicrobial infections. unexplained combination of negative cultures with persistent distressing both patients clinicians. Given broad appreciation advantages provided by rapid testing (e.g., for COVID-19 or influenza A), a rapid, accurate diagnostic test needed deliver timely treatment seeking care that optimizes antibiotic stewardship. Herein, we discuss progress being made toward an accessible, (i.e., within hours), assay clinically useful treating clinician timeframe growth rate pathogen(s)). New emerging often overlooked current techniques reviewed.

Language: Английский

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Pooled Antibiotic Susceptibility Testing Performs Within CLSI Standards for Validation When Measured Against Broth Microdilution and Disk Diffusion Antibiotic Susceptibility Testing of Cultured Isolates

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1214 - 1214

Published: Dec. 14, 2024

Background/Objectives: While new methods for measuring antimicrobial susceptibility have been associated with improved patient outcomes, they should also be validated using standard protocols error rates and other test metrics. The objective of this study was to validate a novel assay complicated recurrent urinary tract infections (UTIs): pooled antibiotic testing (P-AST). This compared broth microdilution (BMD) disk diffusion (DD), following Clinical Laboratory Standards Institute (CLSI) guidelines assessment agreement. Methods: analyzed consecutive fresh clinical urine specimens submitted UTI diagnostic testing. Upon receipt, the samples were subjected in parallel culture multiplex polymerase chain reaction (M-PCR) microbial identification quantification. Specimens same monomicrobial non-fastidious bacteria detected by both M-PCR (SUC) underwent (AST) P-AST Analysis undertaken assess presence heteroresistance P-AST-resistant BMD/DD consensus-susceptible results. Results: performance measures without correction showed essential agreement (EA%) ≥90%, very major errors (VMEs) <1.5%, (MEs) <3.0% P-AST, all meeting threshold established CLSI AST. categorical (CA%) met acceptable criteria (>88%), as majority minor (mEs) decreased <1.0% when accounted for. Conclusions: methodology is within parameters criteria.

Language: Английский

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Interleukins in Urine and Blood as Markers of Infection and as Risk Factors for Systemic Conditions

European Urology Focus, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

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Comparing Prescribing Behaviors and Clinician Experiences Between Multiplex PCR/Pooled Antibiotic Susceptibility Testing and Standard Urine Culture in Complicated UTI Cases

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(23), P. 7453 - 7453

Published: Dec. 7, 2024

We aimed to compare the prescribing behavior and clinical experience of urology providers when using combined multiplex polymerase chain reaction (M-PCR)/Pooled Antibiotic Susceptibility Testing (P-AST) diagnostic test versus standard urine culture (SUC) in same set patients previously reported have improved outcomes with M-PCR/P-AST.

Language: Английский

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Noninferiority of Multiplex Polymerase Chain Reaction Compared to Standard Urine Culture for Urinary Tract Infection Diagnosis in Pediatric Patients at Hackensack Meridian Health Children’s Hospital Emergency Department

Pediatric Health Medicine and Therapeutics, Journal Year: 2024, Volume and Issue: Volume 15, P. 351 - 364

Published: Nov. 1, 2024

To establish the noninferiority of rapid and sensitive multiplex polymerase chain reaction (M-PCR) method versus standard urine culture (SUC) in pediatric urinary tract infection (UTI) diagnostic testing.

Language: Английский

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