Phytochemistry and GCMS Analysis of Ethanol and Aqueous Stembark Extracts of Detarium microcarpum Guill. & Perr. Fabaceae
Sciences of Phytochemistry,
Journal Year:
2025,
Volume and Issue:
4(1), P. 9 - 19
Published: Jan. 9, 2025
The
therapeutic
applications
of
medicinal
plants
in
the
treatment
various
diseases
can
be
attributed
to
their
diverse
phytochemical
constituents.
This
study
aimed
investigate
composition
aqueous
and
ethanol
stem
bark
extracts
Detarium
microcarpum.
Qualitative
quantitative
analyses
were
conducted
determine
presence
concentrations
phytochemicals,
followed
by
identification
phytoconstituents
using
gas
chromatography-mass
spectrometry
(GC-MS).
extract
was
found
contain
saponins
(27.11
±
0.22%)
flavonoids
(47.33
0.70%
),
with
alkaloids,
steroids,
glycosides,
terpenoids
absent.
In
contrast,
contained
alkaloids
(10.78
0.59%),
(45.11
0.48%),
glycosides
(5.44
(11.00
0.77%),
while
steroids
not
detected.
GC-MS
analysis
revealed
14
compounds
20
extract.
major
constituents
included
hydroperoxide,
1,4-dioxan-2-yl
(58.32%),
1,2,3-benzenetriol
(16.44%),
cis-p-coumaric
acid
(11.05%).
extract,
predominant
coumarin
(29.41%),
benzofuran
(17.23%),
catechol
(9.23%).
identified
may
serve
as
potential
synthetic
templates
for
development
novel
agents
targeting
diseases.
supports
ethnomedicinal
use
D.
microcarpum
provides
a
scientific
basis
its
role
traditional
medical
practices.
Language: Английский
GC-MS Analysis, Antioxidant, Antidiabetic Activity, and ADMET Study of Diospyros mespiliformis Hochst. Ex A. DC. Ebenaceae Stembark
Hacettepe University Journal of the Faculty of Pharmacy,
Journal Year:
2024,
Volume and Issue:
44(3), P. 198 - 219
Published: Aug. 6, 2024
The
present
study
carried
out
GC-MS
analysis,
antioxidant,
antidiabetic,
and
ADMET
of
the
crude
ethanol
extract
(CRE),
ethyl
acetate
(EAF),
aqueous
(AQF)
fractions
Diospyros
mespiliformis
(DM).
Grandiflorenic
cis,
cis-linoleic
acids
were
most
abundant
59
40
compounds
identified
in
EAF
AQF,
respectively.
CRE
exhibited
significantly
(p<
0.05)
higher
total
antioxidant
capacity
than
AQF.
Furthermore,
a
(p
<
percentage
inhibition
via
ferric
thiocyanate
assay
All
extracts
showed
lower
malondialdehyde
concentrations
AA
thiobarbituric
acid
assay.
Diazoprogesterone
lowest
binding
affinity
constant,
interacting
with
myeloperoxidase
(MPO),
xanthine
(XO),
11-β-hydroxysteroid
dehydrogenase
(HSD1),
sirtuin
6
(SIRT6).
molecular
dynamics
simulations
residue
fluctuations
diazoprogesterone
docked
complexes
highest
observed
at
Ser42,
Cys1325,
Ser281,
Leu78
for
MPO,
XO,
HSD1,
SIRT6,
Moreover,
was
predicted
to
possess
good
properties.
Conclusively,
DM
possesses
significant
antidiabetic
potential,
containing
that
might
be
source
novel
therapeutics
against
oxidative
stress
diabetes.
Language: Английский
An In Vitro Assessment of the Antioxidant Activity of Detarium microcarpum Guill. & Perr. Fabaceae
Sciences of Phytochemistry,
Journal Year:
2024,
Volume and Issue:
3(2), P. 114 - 122
Published: Nov. 27, 2024
Medicinal
plants
are
regarded
as
important
sources
of
exogenous
antioxidants
due
to
their
phytoconstituents’
free
radical
scavenging
potential.
The
present
study
explores
the
phytoconstituents
and
antioxidant
activity
n-hexane
(HFDM)
chloroform
(CFDM)
fractions
Detarium
microcarpum
for
potential
use
in
phytotherapy
oxidative
stress-linked
ailments.
were
qualitatively
determined,
while
was
determined
by
vitro
assays.
Alkaloids,
saponins,
steroids,
flavonoids
detected
both
fractions,
glycosides
terpenoids
absent.
HFDM
(55.40
±
2.66
AAE
µg/mL)
showed
a
significantly
higher
total
capacity
than
CFDM
(34.84
1.22
µg/mL,
p<0.05)
at
tested
concentration
(100
(57.84
2.16
exhibited
ferric
reducing
power
(46.11
1.91
µg/mL).
In
thiocyanate
assay,
there
no
significant
(p>0.05)
difference
between
(65.59
2.32%)
(77.42
2.95%).
However,
percentage
inhibition
ascorbic
acid
(9.68
5.41%,
p<0.05).
Moreover,
(0.21
0.01
nmol/mL)
lower
MDA
(0.66
0.06
AA
(0.46
0.02
nmol/mL).
Additionally,
promising
potential,
which
might
be
attributed
identified
phytochemicals
that
have
applications
diseases.
Language: Английский
Phytoconstituents and In Vitro Free Radical Scavenging Potential of n-Hexane and Aqueous Fractions of Cucurbita maxima and Leptadenia hastata
Sciences of Pharmacy,
Journal Year:
2024,
Volume and Issue:
3(4), P. 193 - 202
Published: Nov. 11, 2024
The
present
study
explored
the
phytoconstituents
and
radical
scavenging
activity
of
respective
n-hexane
aqueous
fractions
Cucurbita
maxima
(CMHF
CMAF)
Leptadenia
hastata
(LHHF
LHAF)
for
potential
application
in
oxidative
stress-related
ailments.
were
qualitatively
determined
characterized
using
Fourier-transform
Infrared
(FTIR),
while
antioxidant
was
vitro.
Alkaloids
only
C.
L.
hastata,
saponins,
steroids,
flavonoids
detected
all
fractions.
FTIR
revealed
presence
functional
groups,
including
alcohols,
sulfonates,
alkenes,
alkanes,
amines,
aromatics
both
plant
LHHF
(35.53
±2.11
ascorbic
acid
(AA)
equivalent
µg/mL)
exhibited
a
significantly
(p<0.05)
higher
total
reducing
power
(TRP)
than
other
CMHF
(69.11
±2.56
AAE
demonstrated
capacity
(TAC)
For
ferric
thiocyanate
(FTC)
assay,
highest
inhibition
by
(79.78
±
3.24%),
AA
(26.46
2.12%),
(69.77
3.16%),
CMAF
(43.80
2.12%).
In
thiobarbituric
lowest
MDA
concentration
(0.07
±0.01
nmol/mL),
lower
acid.
Conclusively,
fraction
plants
presents
sources
novel
compounds
with
significant
free
anti-lipid
peroxidation
activities,
applicable
ailments
linked
to
stress.
Language: Английский