Alternative Excipients for Protein Stabilization in Protein Therapeutics: Overcoming the Limitations of Polysorbates

Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(12), P. 2575 - 2575

Published: Nov. 23, 2022

Given their safety and efficiency in protecting protein integrity, polysorbates (PSs) have been the most widely used excipients for stabilization of therapeutics years. In recent decades, however, there numerous reports about visible or sub-visible particles PS-containing biotherapeutic products, which is a major quality concern parenteral drugs. Alternative that are safe administration, efficient different drugs against various stress conditions, effective high-concentrated liquid formulations, stable under storage conditions duration product's shelf-life, compatible with other formulation components primary packaging highly sought after. The aim this paper to review potential alternative from families, including surfactants, carbohydrate- amino acid-based excipients, synthetic amphiphilic polymers, ionic liquids enable stabilization. For each category, important characteristics such as ability stabilize proteins thermal mechanical stresses, current knowledge related profile interactions components, debated. Based on provided information detailed discussion thereof, may pave way identification development

Language: Английский

---

Osmolytes: Wonder molecules to combat protein misfolding against stress conditions

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 234, P. 123662 - 123662

Published: Feb. 15, 2023

Language: Английский

---

Genome-wide characterization and functional analysis of class III peroxidase gene family in soybean reveal regulatory roles of GsPOD40 in drought tolerance

Genomics, Journal Year: 2021, Volume and Issue: 114(1), P. 45 - 60

Published: Nov. 20, 2021

Class III peroxidases (PODs) are plant-specific glycoproteins, that play essential roles in various plant physiological processes and defence responses. To date, scarce information is available about the POD gene family soybean. Hence, present study first comprehensive report genome-wide characterization of GmPOD soybean (Glycine max L.). Here, we identified a total 124 genes soybean, unevenly distributed across genome. Phylogenetic analysis classified them into six distinct sub-groups (A-F), with one specific subgroup. Exon-intron motif suggested existence structural functional diversity among sub-groups. Duplication 58 paralogous pairs; segmental duplication positive/Darwinian selection were observed as major factors involved evolution GmPODs. Furthermore, RNA-seq revealed 23 out GmPODs showed differential expression between drought-tolerant drought-sensitive genotypes under stress conditions; however, two (GmPOD40 GmPOD42) maximum deregulation all contrasting genotypes. Overexpression (OE) lines GsPOD40 considerably higher drought tolerance compared to wild type (WT) plants treatment. Moreover, OE enhanced photosynthesis enzymatic antioxidant activities stress, resulting alleviation ROS induced oxidative damage. by regulating key biochemical pathways response. Lastly, results our will greatly assist further GsPODs growth

Language: Английский

---

The ability of trimethylamine N-oxide to resist pressure induced perturbations to water structure

Communications Chemistry, Journal Year: 2022, Volume and Issue: 5(1)

Published: Sept. 28, 2022

Trimethylamine N-oxide (TMAO) protects organisms from the damaging effects of high pressure. At molecular level both TMAO and pressure perturb water structure but it is not understood how they act in combination. Here, we use neutron scattering coupled with computational modelling to provide atomistic insight into under at 4 kbar presence absence TMAO. The data reveal that resists pressure-induced perturbation structure, particularly retaining a clear second solvation shell, enhanced hydrogen bonding between molecules strong - bonds. We calculate an 'osmolyte protection' ratio which TMAO-induced energy changes effectively cancel out. Remarkably this translates across scales organism level, matching observed concentration dependence muscle tissue as function depth. Osmolyte protection may therefore offer mechanism for macroscale survival life extreme environments.

Language: Английский

---

Bacillus sp. and Pseudacidovorax intermedius colonization effect on biochemical and metabolites expression in drought-stressed Sorghum bicolor (L.) Moench

Plant Stress, Journal Year: 2024, Volume and Issue: 11, P. 100424 - 100424

Published: March 1, 2024

Drought stress severely affects the physiology and metabolism of plants. Fortunately, using plant growth-promoting bacteria can provide an environmentally friendly solution. Hence, present experiment was conducted on sorghum for drought amelioration by utilizing Bacillus sp. Pseudacidovorax intermedius bacterization to seeds under greenhouse conditions. induced withholding water after 52 days emergence (flowering stage). However, inoculation could significantly improve tolerance capacity through production different antioxidant enzymes metabolites in plants recovery The results declared that reduced membrane leakage 37% minimizing malondialdehyde (49 & 47%) hydrogen peroxide (32 36%) content. In addition protecting leakage, bacterial positively modulates namely catalase, ascorbate peroxidase, glutathione superoxide dismutase, phenol content system. Metabolomic revealed consortia up regulate carboxylic acid, organic, amino acids biomolecules expression inside inoculated study concluded is assured technology mitigating effect compounds, leaf during Further, research needs comprehend underlying molecular mechanism bacterial-induced drought-tolerant It will also create a road map develop bestselling bioinoculant climate-smart agriculture.

Language: Английский

---

Osmolyte-specific counteraction of ethanol-induced aggregation and structure-function distortion of catalase

Journal of Molecular Liquids, Journal Year: 2024, Volume and Issue: 400, P. 124506 - 124506

Published: March 16, 2024

Language: Английский

---

Taurine, a Naturally Occurring Amino Acid, as a Physical Stability Enhancer of Different Monoclonal Antibodies

The AAPS Journal, Journal Year: 2024, Volume and Issue: 26(1)

Published: Feb. 14, 2024

Language: Английский

---

Proline Accumulation in Pollen Grains as Potential Target for Improved Yield Stability Under Salt Stress

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: Oct. 28, 2020

Seed yield, a major determinant for the commercial success of grain crops, critically depends on pollen viability, which is dramatically reduced by environmental stresses, such as drought, salinity, and extreme temperatures. Salinity, in particular, problem crop yield known to affect about 20% all arable land cause huge economic losses worldwide. Flowering plants are particularly sensitive stress during sexual reproduction, even short exposure stressing conditions can severely hamper reproductive success, thus reduce yield. Since proline required fertility accumulates plant tissues response different abiotic role protection under salt be envisaged. In this perspective, we analyze old new data evaluate importance development saline conditions, discuss possibility raising levels grains biotechnological strategy stabilize seed presence stress. The overall confirm that necessary preserve limit loss stressful conditions. However, at present, have not enough conclude whether or over wildtype effectively ameliorate further work still required.

Language: Английский

---

Evidence of microplastics release from polythene and paper cups exposed to hot and cold: A case study on the compromised kinetics of catalase

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 454, P. 131496 - 131496

Published: April 27, 2023

Language: Английский

---

Advancing Cu/Zn Superoxide Dismutase (SOD1) production in Pichia pastoris : challenges, strategies, current research status, and future directions

Preparative Biochemistry & Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 21

Published: March 27, 2025

Cu/Zn Superoxide Dismutase (SOD1) plays a critical role in alleviating oxidative stress by catalyzing the conversion of superoxide radicals into oxygen and hydrogen peroxide. This review presents an in-depth analysis challenges strategies involved optimizing SOD1 production, with focus on Pichia pastoris as expression system. Key approaches such strategic selection vectors, codon optimization, fine-tuning fermentation parameters to maximize yield are thoroughly explored. Advances protein engineering, co-expression molecular chaperones, use stabilizers additives also examined for their improving stability functionality. The highlights significant biomedical industrial applications overexpressed thermostable SOD1, uncovering novel opportunities therapeutic interventions biotechnological innovations. Additionally, emerging technologies omics-based approaches, advanced engineering tools, alternative host systems discussed, offering new avenues future research. comprehensive underscores transformative potential positioning it at forefront scientific technological advancements.

Language: Английский

---