Daptomycin Liposomes Exhibit Enhanced Activity against Staphylococci Biofilms Compared to Free Drug

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(4), P. 459 - 459

Published: March 26, 2024

The purpose of the present study was to investigate anti-staphylococcal activity liposomal daptomycin against four biofilm-producing S. aureus and epidermidis clinical strains, three which are methicillin-resistant. Neutral negatively charged daptomycin-loaded liposomes were prepared using methods, namely, thin-film hydration (TFH), a dehydration–rehydration vesicle (DRV) method, microfluidic mixing (MM); moreover, they characterized for drug encapsulation (EE%), size distribution, zeta-potential, stability, release, integrity. Interestingly, whilst loading in THF DRV nanosized (by extrusion) vesicles around 30–35, very low (~4%) possible MM vesicles, requiring further explanatory investigations. Liposomal protected from degradation preserved its bioactivity. Biofilm mass (crystal violet, CV), biofilm viability (MTT), growth curve (GC) assays evaluated antimicrobial neutral daptomycin-liposomes towards planktonic bacteria biofilms. exhibited dramatically enhanced inhibition bacterial (compared free drug) all species studied, while totally inactive. prevention treatment studies revealed high antibiofilm daptomycin. more active negative charge ones treating established Planktonic as well matured biofilms daptomycin-susceptible, methicillin-resistant Staphylococcus (MRSA) (MRSE) strains almost completely eradicated by liposomal-daptomycin, indicating need their exploration therapeutics.

Language: Английский

Daptomycin Liposomes Exhibit Enhanced Activity against Staphylococci Biofilms Compared to Free Drug

Published: Feb. 26, 2024

The purpose of the present study was to investigate anti-staphylococcal activity liposomal daptomycin, against four biofilm-producing S. aureus and epidermidis clinical strains, three which are methicillin resistant. Neutral negatively charged Daptomycin-loaded liposomes were prepared by methods thin film hydration (TFH), DRV microfluidic mixing (MM), characterized for drug encapsulation (EE%), size distribution, zeta-potential, vesicle stability, release integrity. Interestingly, whilst loading in THF nanosized (by extrusion) vesicles around 30-35, very low (~4%) possible MM vesicles, requiring further explanatory investigations. Liposomal protected daptomycin from degradation preserve its bioactivity. Quantitative microtiter plate (crystal violet, CV), methylthiazoltetrazolium (MTT), growth curve (GC) assays evaluated antimicrobial neutral negative charge daptomycin-liposomes towards planktonic bacteria biofilms. exhibited dramatically enhanced inhibition (compared free drug) all species studied, while totally inactive. Biofilm prevention treatment studies revealed high antibiofilm daptomycin. more active ones treating established Planktonic as well matured biofilms susceptible MRSE MRSA strains almost completely eradicated liposomal-daptomycin indicating need their exploration therapeutics.

Language: Английский

Inhaled combined antibacterials against biofilm-forming antibiotic-resistant bacteria for the management of pulmonary bacterial infections

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 106555 - 106555

Published: Dec. 1, 2024

Language: Английский