Responses of sediment resistome, virulence factors and potential pathogens to decades of antibiotics pollution in a shrimp aquafarm

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 794, P. 148760 - 148760

Published: June 30, 2021

Language: Английский

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Gnotobiotic rainbow trout (Oncorhynchus mykiss) model reveals endogenous bacteria that protect against Flavobacterium columnare infection

PLoS Pathogens, Journal Year: 2021, Volume and Issue: 17(1), P. e1009302 - e1009302

Published: Jan. 29, 2021

The health and environmental risks associated with antibiotic use in aquaculture have promoted bacterial probiotics as an alternative approach to control fish infections vulnerable larval juvenile stages. However, evidence-based identification of is often hindered by the complexity bacteria-host interactions host variability microbiologically uncontrolled conditions. While these difficulties can be partially resolved using gnotobiotic models harboring no or reduced microbiota, most host-microbe interaction studies are carried out animal little relevance for farming. Here we studied host-microbiota-pathogen a germ-free model rainbow trout ( Oncorhynchus mykiss ), one widely cultured salmonids. We demonstrated that larvae raised sterile conditions displayed significant difference growth after 35 days compared conventionally-raised larvae, but were extremely sensitive infection Flavobacterium columnare , common freshwater pathogen causing major economic losses worldwide. Furthermore, re-conventionalization 11 culturable species from conventional microbiota conferred resistance F . infection. Using mono-re-conventionalized trout, identified this protection determined commensal strain displaying antibacterial activity against Finally, suitable both endogenous exogenous probiotic strains protecting teleostean hosts This study therefore establishes ecologically-relevant host-pathogen colonization farmed fish.

Language: Английский

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Potential aquatic environmental risks of trifloxystrobin: Enhancement of virus susceptibility in zebrafish through initiation of autophagy

动物学研究, Journal Year: 2021, Volume and Issue: 42(3), P. 339 - 349

Published: Jan. 1, 2021

Chronic pollution in aquatic ecosystems can lead to many adverse effects, including a greater susceptibility pathogens among resident biota. Trifloxystrobin (TFS) is strobilurin fungicide widely used Asia control soybean rust. However, it has the potential enter ecosystems, where may impair fish resistance viral infections. To explore environmental risks of TFS, we characterized antiviral capacities chronically exposed TFS and subsequently infected with spring viraemia carp virus (SVCV). Although exhibited no significant cytotoxicity at tested concentrations during challenge, SVCV replication increased significantly time-dependent manner within epithelioma papulosum cyprini (EPC) cells zebrafish 25 μg/L TFS. Results showed that highest load was more than 100-fold controls. Intracellular biochemical assays indicated autophagy induced by associated changes included an increase autophagosomes, conversion LC3-II, accumulation Beclin-1, degradation P62 EPC zebrafish. In addition, markedly decreased expression phosphorylation mTOR, indicating activation be mTOR-mediated pathway. This study provides new insights into mechanism immunosuppressive effects on non-target hosts suggests existence environments contribute outbreaks diseases.

Language: Английский

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OTUs and ASVs Produce Comparable Taxonomic and Diversity from Shrimp Microbiota 16S Profiles Using Tailored Abundance Filters

Genes, Journal Year: 2021, Volume and Issue: 12(4), P. 564 - 564

Published: April 13, 2021

The interplay between shrimp immune system, its environment, and microbiota contributes to the organism's homeostasis optimal production. metagenomic composition is typically studied using 16S rDNA profiling by clustering amplicon sequences into operational taxonomic units (OTUs) and, more recently, sequence variants (ASVs). Establish compatibility of taxonomy, α, β diversity described both methods necessary compare past future studies. Here, we used identical survey V3 hypervariable-region 97% 99% OTUs ASVs assess hepatopancreas intestine L. vannamei from two ponds under standardized rearing conditions. We found that applying filters retain clusters >0.1% total abundance per sample enabled a consistent taxonomy comparison while preserving >94% reads. three sets turned comparable at family level, whereas identity OTU set produced divergent genus species profiles. Interestingly, detection organ pond variations was robust method's choice, producing α β-diversity For comparisons on studies, strongly recommend be compared level or use OTUs, tailored frequency filters.

Language: Английский

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Parental exposure to sulfamethazine and nanoplastics alters the gut microbial communities in the offspring of marine madaka (Oryzias melastigma)

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 423, P. 127003 - 127003

Published: Aug. 21, 2021

Language: Английский

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