Disulfidptosis as a key regulator of glioblastoma progression and immune cell impairment

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 30, 2025

Background Glioblastoma, associated with poor prognosis and impaired immune function, shows potential interactions between newly identified disulfidptosis mechanisms T cell exhaustion, yet these remain understudied. Methods Key genes were using Lasso regression, followed by multivariate analysis to develop a prognostic model. Single-cell pseudotemporal explored T-cell exhaustion (Tex) signaling in differentiation. Immune infiltration was assessed via ssGSEA, while transwell assays immunofluorescence examined the effects of disulfidptosis-Tex on glioma behavior response. Results Eleven found critical for glioblastoma survival outcomes. This gene set underpinned model predicting patient prognosis. showed high activity endothelial cells. Memory populations linked genes. SMC4 inhibition reduced LN299 migration increased chemotherapy sensitivity, decreasing CD4 CD8 activation. Conclusions Disulfidptosis-Tex are pivotal progression interactions, offering new avenues improving anti-glioblastoma therapies through modulation exhaustion.

Language: Английский

Potential Biomarkers for IDH-Mutant and IDH-Wild-Type Glioblastomas: A Single-Center Retrospective Study

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(7), P. 2518 - 2518

Published: April 7, 2025

Background/Objectives: Glioblastoma ranks among the most aggressive brain tumors, with poor prognosis. Currently, there are insufficient data regarding prognostic value of isocitrate dehydrogenase (IDH) mutation status and inflammatory markers. This study demonstrates IDH preoperative markers in glioblastoma. Methods: single-center retrospective encompassed 66 glioblastoma patients who had surgical treatment our institution from January 2020 to March 2022. The were categorized into two groups: IDH-mutant (n = 30) IDH-wild-type 36). We made a comparative assessment demographic characteristics, clinical parameters, blood survival outcome across groups. Statistical analyses included Kaplan-Meier curves, ROC analysis, multivariate Cox regression. Results: group demonstrated significantly lower mean age (53.93 ± 12.00) compared wild-type (62.39 10.12) (p 0.003). Median overall was notably longer group, at 16.0 months, versus 6.5 months 0.030). An elevated neutrophil/lymphocyte ratio above 3.39 (sensitivity 95.12%, specificity 52.0%) platelet/lymphocyte exceeding 136.25 80.49%, 64.0%) associated regression analysis identified (HR 2.84, 95% CI: 1.56-5.18) NLR 1.84, 1.16-2.92) as independent factors. Conclusions: show that glioblastomal have poorer In this case, metrics seem be supplied some biomarkers for expansion disease predicting likely outcomes.

Language: Английский

Sennoside A represses the malignant phenotype and tumor immune microenvironment of non-small cell lung cancer cells by inhibiting the TRAF6/NF-κB pathway

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

Language: Английский