Evaluation of the Toxicological Efficacy of Protein Hydrolysates From Camel Milk β-Casein Against Different Types of Cancer Cell Lines in Vitro DOI Open Access
Sharaf Ali Hadi Al-Shaikh,

Kifah Saed Doosh

IOP Conference Series Earth and Environmental Science, Journal Year: 2024, Volume and Issue: 1371(6), P. 062027 - 062027

Published: July 1, 2024

Abstract This study aimed to isolate and purify the β-CN protein from camel milk using ion exchange chromatography gel filtration techniques verify its purity polyacrylamide electrophoresis (PAGE), Then prepare hydrolyzates of isolated by enzymatic hydrolysis enzyme pepsin trypsin their mixture in a ratio (1:1). The cytotoxicity degraders was studied conducting an MTT test determine toxic effect ability inhibit growth cancer cells represented HepG2 liver cells, PC3 prostate MCF-7 breast comparing them with normal HdFn vitro. results showed presence two peaks for on DEAE-Cellulose one peak Sephadex G-100 column appearance clear pure band gel. highest decomposition rate due synergistic action at 1:1 amounted 52.32%. also that have effective inhibitory cell concentration 400µg/mL, as inhibition reached (45.9, 59.5 63.3%) types, respectively. Based test, line chosen it is most affected under study. Cytotoxicity evaluated performing HCS detect some changes may occur measuring cellular indicators, including Viable Cell Count (VCC), total Nuclear Intensity (TNI), Membrane Permeability (CMP), Mitochondrial Potential (MMP), Cytochrome C releasing (CC) level. indicated decrease number (VCC) especially concentrations 100 200 µg/mL, 31.53 47.58%, respectively, increase significantly significance level (P≤0.05), 39.10 51.91%, there (MMP) 20.47 27.39%, significant release 16.16 25.84%, It can be concluded above high activity against

Language: Английский

Synergistic Antiproliferative Effect of Linagliptin-Metformin Combination on the Growth of Hela Cancer Cell Line DOI Creative Commons
A. M. Abu Ahmed,

Ahmed Nihad,

Ghaeeb Sabreen

et al.

Journal of cancer research updates, Journal Year: 2025, Volume and Issue: 14, P. 12 - 23

Published: Feb. 18, 2025

This in-vitro study explores the cytotoxic properties of linagliptin-metformin combination on cervical cancer cells and examines synergistic interaction between two drugs. An MTT assay was used to explore anti-cancer effects mixture a cell line (HeLa line) across 24 72-hour incubation periods. The concentrations metformin, linagliptin, their ranged from 0.1 1000 µg/ml. while in were kept at fifty percentage individually drug. included an estimated index value (CI) dosage reduction (DRI) ascertain possibility effect combined drugs safety. finding exhibited that all studied drugs- mixture- inhibited growth with superior efficacy over individual Inhibition patterns directly proportional drug's concentration time. revealed mixture's metformin linagliptin synergistic. dose lower drug required than when indicating greater potential mixture. findings MTT, CI, DRI indicate is effective, safer, promising anticancer therapy for cancer. Conclusion: combination. highlights effective concentrations, paving way further research using these treatment.

Language: Английский

Citations

0
The Potential Role of HDAC1 and HDAC3 Immunoexpression in P53 Downregulation and Tumor Aggressiveness of Colon and Rectum Carcinomas Patients DOI Creative Commons
Yasir Qaddoori, Ahmed S. Al-Khafaji, Basim Mohammed Khashman

et al.

Experimental Oncology, Journal Year: 2025, Volume and Issue: 46(4), P. 393 - 401

Published: Feb. 20, 2025

Background. Colorectal cancer, ranking second place in global cancer mortality, arises from diverse causes. There is growing recognition of the substantial involvement epigenetic modifications histones at DNA level occurrence CRC. Aim. To assess expression p53, HDAC1, and HDAC3 proteins a cohort CRC patients to analyze potential relationship between their stages progression. Materials Methods. The retrospective investigation was carried out on 95 paraffin-embedded tissue samples. assessed immunohistochemically. Results. Notably, p53 protein samples exhibited prominent correlation with both HDAC1 (p < 0.001, rho = 0.522) 0.411), as well advanced TNM staging 0.002, 0.313). Downregulation correlated underexpressed HDAC3. Nevertheless, observed significant negative age patients. Conclusion. data HDACs-p53 co-expression suggest possible mechanism interaction these proteins.

Language: Английский

Citations

0
Evaluation of the Toxicological Efficacy of Protein Hydrolysates From Camel Milk β-Casein Against Different Types of Cancer Cell Lines in Vitro DOI Open Access
Sharaf Ali Hadi Al-Shaikh,

Kifah Saed Doosh

IOP Conference Series Earth and Environmental Science, Journal Year: 2024, Volume and Issue: 1371(6), P. 062027 - 062027

Published: July 1, 2024

Abstract This study aimed to isolate and purify the β-CN protein from camel milk using ion exchange chromatography gel filtration techniques verify its purity polyacrylamide electrophoresis (PAGE), Then prepare hydrolyzates of isolated by enzymatic hydrolysis enzyme pepsin trypsin their mixture in a ratio (1:1). The cytotoxicity degraders was studied conducting an MTT test determine toxic effect ability inhibit growth cancer cells represented HepG2 liver cells, PC3 prostate MCF-7 breast comparing them with normal HdFn vitro. results showed presence two peaks for on DEAE-Cellulose one peak Sephadex G-100 column appearance clear pure band gel. highest decomposition rate due synergistic action at 1:1 amounted 52.32%. also that have effective inhibitory cell concentration 400µg/mL, as inhibition reached (45.9, 59.5 63.3%) types, respectively. Based test, line chosen it is most affected under study. Cytotoxicity evaluated performing HCS detect some changes may occur measuring cellular indicators, including Viable Cell Count (VCC), total Nuclear Intensity (TNI), Membrane Permeability (CMP), Mitochondrial Potential (MMP), Cytochrome C releasing (CC) level. indicated decrease number (VCC) especially concentrations 100 200 µg/mL, 31.53 47.58%, respectively, increase significantly significance level (P≤0.05), 39.10 51.91%, there (MMP) 20.47 27.39%, significant release 16.16 25.84%, It can be concluded above high activity against

Language: Английский

Citations

0