Exploring and expanding the natural chemical space of bacterial diterpenes DOI Creative Commons

Xiuting Wei,

Wenbo Ning, Caitlin A. McCadden

et al.

Published: Aug. 30, 2024

Terpenoids are the largest family of natural products but relatively rare in bacteria. Genome mining reveals wide- spread prevalence terpene synthases, enzymes responsible for constructing hydrocarbon skeletons, Using an engineered E. coli diterpene production system, we screened 334 diverse synthases from 8 phyla, 17 classes, and 83 genera bacteria found that 125 (37%) were active as synthases. Isolation structural elucidation 28 bacterial diterpenes 31 TSs revealed three new skeletons previously unseen nature, known other organisms (e.g., coral, sponge, algae) with unknown biosynthetic pathways, fungi, plants) have not been seen bacteria, stereochemical isomers diterpenes. This study will lead to novel products, advances terpenoid biosynthesis roles these products.

Language: Английский

Exploring and expanding the natural chemical space of bacterial diterpenes DOI Creative Commons

Xiuting Wei,

Wenbo Ning, Caitlin A. McCadden

et al.

Published: Aug. 30, 2024

Terpenoids are the largest family of natural products but relatively rare in bacteria. Genome mining reveals wide- spread prevalence terpene synthases, enzymes responsible for constructing hydrocarbon skeletons, Using an engineered E. coli diterpene production system, we screened 334 diverse synthases from 8 phyla, 17 classes, and 83 genera bacteria found that 125 (37%) were active as synthases. Isolation structural elucidation 28 bacterial diterpenes 31 TSs revealed three new skeletons previously unseen nature, known other organisms (e.g., coral, sponge, algae) with unknown biosynthetic pathways, fungi, plants) have not been seen bacteria, stereochemical isomers diterpenes. This study will lead to novel products, advances terpenoid biosynthesis roles these products.

Language: Английский

Citations

1