The current economics and throughput of single cell proteomics by liquid chromatography mass spectrometry DOI Creative Commons
Amanda L. Smythers, Benjamin C. Orsburn

Published: June 11, 2024

Single cell proteomics by mass spectrometry (SCP) is an emerging field of study that has captured the interest and imagination biologists in a wide array disciplines. In pursuit this new dizzying technologies techniques have demonstrated ability to quantify hundreds few thousand proteins single mammalian cells typical size. One striking characteristic these methods range relative costs associated with analysis each cell. We attempted estimate cost per across 17 different studies based on quotes we obtained for hardware, reagents instrument support plans relation number can be analyzed day. Before including labor or facilities, find analyze size from less than <$2 over $50 The increase appears directly related decrease throughput as measured theoretical maximum Perhaps most surprising observation average year. This when compared emergence RNA sequencing where increased, cost/cell decreased exponentially first 7 years field’s emergence. While made many assumptions obtain estimates, hope will informative scientists interested obtaining SCP data spectrometrists who are considering entering field. provided spreadsheet simple calculator supplemental allow others adjust our calculations other variables which inevitably described future.

Language: Английский

Formaldehyde Fixation Helps Preserve the Proteome State during Single-Cell Proteomics Sample Processing and Analysis DOI
Ilaria Piga, Claire Koenig, Maico Lechner

et al.

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Mass spectrometry-based single-cell proteomics (SCP) is gaining momentum but remains limited to a few laboratories due the high costs and specialized expertise required. The ability send samples core facilities would benefit nonspecialist popularize SCP for biological applications. However, no methods have been tested in "freeze" proteome state while maintaining cell integrity transfer between or prolonged sorting using fluorescence-activated (FACS). This study evaluates whether short-term formaldehyde (FA) fixation can maintain states. We demonstrate that FA does not substantially affect protein recovery, even without heating strong detergents, maintains analytical depth compared with classical workflows. Fixation also preserves drug-induced specific perturbations of abundance during sample preparation analysis. Our findings suggest facilitate by enabling shipping sorting, potentially democratizing access technology expanding its application research, thereby accelerating discoveries biology personalized medicine.

Language: Английский

Citations

0
The current economics and throughput of single cell proteomics by liquid chromatography mass spectrometry DOI Creative Commons
Amanda L. Smythers, Benjamin C. Orsburn

Published: June 11, 2024

Single cell proteomics by mass spectrometry (SCP) is an emerging field of study that has captured the interest and imagination biologists in a wide array disciplines. In pursuit this new dizzying technologies techniques have demonstrated ability to quantify hundreds few thousand proteins single mammalian cells typical size. One striking characteristic these methods range relative costs associated with analysis each cell. We attempted estimate cost per across 17 different studies based on quotes we obtained for hardware, reagents instrument support plans relation number can be analyzed day. Before including labor or facilities, find analyze size from less than <$2 over $50 The increase appears directly related decrease throughput as measured theoretical maximum Perhaps most surprising observation average year. This when compared emergence RNA sequencing where increased, cost/cell decreased exponentially first 7 years field’s emergence. While made many assumptions obtain estimates, hope will informative scientists interested obtaining SCP data spectrometrists who are considering entering field. provided spreadsheet simple calculator supplemental allow others adjust our calculations other variables which inevitably described future.

Language: Английский

Citations

2