Moldable plastics (polycaprolactone) can be acutely toxic to developing zebrafish and activate nuclear receptors in mammalian cells DOI Creative Commons
Bryan D. James, Alexander V. Medvedev, Sergei S. Makarov

et al.

Published: April 12, 2024

Popularized on social media, hand-moldable plastics are formed by consumers into tools, trinkets, and dental prosthetics. Despite the anticipated dermal oral contact, manufacturers share little information with about these materials. Inherent to their function, moldable pose a risk of exposure unknown leachable substances. We analyzed 12 advertised for modeling applications determined them be polycaprolactone (PCL) or thermoplastic polyurethane (TPU). The bioactivities most popular brands each type polymer were evaluated using zebrafish embryo bioassay. Both products sold as microplastic-sized resin pellets. While water-borne TPU pellets did not affect targeted developmental endpoints at any concentration tested, PCL acutely toxic above 1 pellet/mL. Aqueous leachates demonstrated similar toxicity. Methanolic extracts from assayed bioactivity Attagene FACTORIAL platform. Of 69 measured endpoints, activated nuclear receptors transcription factors xenobiotic metabolism (pregnane X receptor, PXR), lipid (peroxisome proliferator-activated receptor gamma, PPARg), oxidative stress (nuclear factor erythroid 2-related 2, NRF2). By non-targeted high-resolution comprehensive two-dimensional gas chromatography (GC×GC-HRT), we tentatively identified several compounds in methanolic extracts, including oligomers, phenolic antioxidant, residues suspected anti-hydrolysis crosslinking additives. In follow-up bioassay, because its stated high purity, biomedical grade was tested mitigate confounding effects due chemical additives pellets; it elicited comparable acute From orthogonal complementary experiments, suggest that toxicity oligomers nanoplastics released rather than These results challenge perceived assumed inertness highlight multiple sources

Language: Английский

Moldable plastics (polycaprolactone) can be acutely toxic to developing zebrafish and activate nuclear receptors in mammalian cells DOI Creative Commons
Bryan D. James, Alexander V. Medvedev, Sergei S. Makarov

et al.

Published: April 12, 2024

Popularized on social media, hand-moldable plastics are formed by consumers into tools, trinkets, and dental prosthetics. Despite the anticipated dermal oral contact, manufacturers share little information with about these materials. Inherent to their function, moldable pose a risk of exposure unknown leachable substances. We analyzed 12 advertised for modeling applications determined them be polycaprolactone (PCL) or thermoplastic polyurethane (TPU). The bioactivities most popular brands each type polymer were evaluated using zebrafish embryo bioassay. Both products sold as microplastic-sized resin pellets. While water-borne TPU pellets did not affect targeted developmental endpoints at any concentration tested, PCL acutely toxic above 1 pellet/mL. Aqueous leachates demonstrated similar toxicity. Methanolic extracts from assayed bioactivity Attagene FACTORIAL platform. Of 69 measured endpoints, activated nuclear receptors transcription factors xenobiotic metabolism (pregnane X receptor, PXR), lipid (peroxisome proliferator-activated receptor gamma, PPARg), oxidative stress (nuclear factor erythroid 2-related 2, NRF2). By non-targeted high-resolution comprehensive two-dimensional gas chromatography (GC×GC-HRT), we tentatively identified several compounds in methanolic extracts, including oligomers, phenolic antioxidant, residues suspected anti-hydrolysis crosslinking additives. In follow-up bioassay, because its stated high purity, biomedical grade was tested mitigate confounding effects due chemical additives pellets; it elicited comparable acute From orthogonal complementary experiments, suggest that toxicity oligomers nanoplastics released rather than These results challenge perceived assumed inertness highlight multiple sources

Language: Английский

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