Deciphering the Energy Use Channels in Soil Organic Matter: Impacts of Long-term Farmyard Manure Addition and Microbial Necromass Revealed by LC-FT-ICR-MS DOI Creative Commons

Konstantin Stumpf,

Carsten Simon, Anja Miltner

et al.

Published: June 24, 2024

Soil organic matter (SOM) plays a central role in the global carbon cycle and contributes to storage of C energy soils. Farmyard manure (FYM) addition arable soils is measure increase SOM content, microbial activity abundance metabolites (e.g., necromass (NM) markers). However, understanding mechanistic links between soil dynamics hampered due chemical complexity SOM. Non-targeted molecular-level methods like liquid chromatography coupled Fourier transform ion cyclotron resonance mass spectrometry (LC-FT-ICR-MS) can be used explore complex SOM, revealing energetic fingerprints long-term changes FYM addition. We compared water-extractable (WEOM) from four experiments with representative WEOM signatures maize, bacterial fungal NM. Long-term increased WEOM, most pronounced polar, unsaturated, oxidised energy-poor compounds. These were linked 2-3-fold bacterial, plant NM signatures. Especially FYM-amended indicated shift dominant use channels. Control showed much lower overlap all NMs, but higher dominance energy-use channels, especially for N-containing A large fraction signals (79% FYM-amended, 94% control soils) was unrelated any three signatures, also mainly responsible nominal oxidation state (NOSC) fertilisation treatments. LC-FT-ICR-MS provided access ~600 novel markers which are readily soluble compositionally distinct classical (ergosterol, aminosugars, etc.). Overall, we highlight insights into contribution by LC-FT-ICR-MS, how it assist constrain compositional impacts on

Language: Английский

Deciphering the Energy Use Channels in Soil Organic Matter: Impacts of Long-term Farmyard Manure Addition and Microbial Necromass Revealed by LC-FT-ICR-MS DOI Creative Commons

Konstantin Stumpf,

Carsten Simon, Anja Miltner

et al.

Published: June 24, 2024

Soil organic matter (SOM) plays a central role in the global carbon cycle and contributes to storage of C energy soils. Farmyard manure (FYM) addition arable soils is measure increase SOM content, microbial activity abundance metabolites (e.g., necromass (NM) markers). However, understanding mechanistic links between soil dynamics hampered due chemical complexity SOM. Non-targeted molecular-level methods like liquid chromatography coupled Fourier transform ion cyclotron resonance mass spectrometry (LC-FT-ICR-MS) can be used explore complex SOM, revealing energetic fingerprints long-term changes FYM addition. We compared water-extractable (WEOM) from four experiments with representative WEOM signatures maize, bacterial fungal NM. Long-term increased WEOM, most pronounced polar, unsaturated, oxidised energy-poor compounds. These were linked 2-3-fold bacterial, plant NM signatures. Especially FYM-amended indicated shift dominant use channels. Control showed much lower overlap all NMs, but higher dominance energy-use channels, especially for N-containing A large fraction signals (79% FYM-amended, 94% control soils) was unrelated any three signatures, also mainly responsible nominal oxidation state (NOSC) fertilisation treatments. LC-FT-ICR-MS provided access ~600 novel markers which are readily soluble compositionally distinct classical (ergosterol, aminosugars, etc.). Overall, we highlight insights into contribution by LC-FT-ICR-MS, how it assist constrain compositional impacts on

Language: Английский

Citations

1