Reproductive Adaptation of Astyanax mexicanus Under Nutrient Limitation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract Reproduction is a fundamental biological process for the survival and continuity of species. Examining changes in reproductive strategies offers valuable insights into how animals have adapted their life histories to different environments. Since reproduction one most energy-intensive processes female animals, nutrient scarcity expected interfere with ability invest gametes. Lately, new model study adaptation limitation has emerged; Mexican tetra Astyanax mexicanus . This fish species exists as two morphs, surface river morph cave-dwelling morph. The dark, biodiversity, nutrient-limited cave environment consequently evolved an impressive starvation resistance. However, limitations this remains poorly understood. Here, we compared breeding activities maternal contributions between laboratory-raised cavefish. We found that cavefish produce clutch sizes eggs larger yolk fish, indicating greater deposition embryos. To systematically characterize compositions, used untargeted proteomics lipidomics approaches analyze protein lipid profiles 2-cell stage embryos increased proportion sphingolipids fish. Additionally, generated transcriptomic ovaries using combination single cell bulk RNA sequencing examine differences contribution. genes essential hormone regulation were upregulated follicular somatic cells evaluate whether these contribute abilities under natural-occurring stress, induced starved Remarkably, maintained breed starvation, whereas largely lost ability. identified insulin-like growth factor 1a receptor ( igf1ra ) potential candidate gene mediating downregulation ovarian development genes, potentially contributing starvation-resistant fertility Taken together, investigated , which will provide adaptations environments extreme deficit.

Language: Английский

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Reproductive Adaptation of Astyanax mexicanus Under Nutrient Limitation

Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Isotopic variability of δ13C and δ15N signals in cave systems: insights from the blind tetra Astyanax mexicanus

Subterranean Biology, Journal Year: 2025, Volume and Issue: 51, P. 31 - 47

Published: March 17, 2025

Stable isotope analysis allows the study of element cycles in ecosystems and trophic ecology. δ 13 C reflects diversity primary productivity, while 15 N is a good indicator levels organisms. Caves have limited resources due to absence light, reducing chains these ecosystems. These extreme conditions impose strong selection pressures on cave-dwelling organisms, known as troglobites, which exhibit specific adaptations such vision pigment loss, metabolic physiological differences with their surface counterparts. The species Astyanax mexicanus corresponds model organism regressive evolution, presents two different ecotypes, widely distributed morph, morph present at least 34 caves three karst regions San Luis Potosí Tamaulipas, Mexico. In study, we characterized seven cave populations A. , corresponding regions: Sierra de El Abra Guatemala, representing distinct genetic cavefish lineages. We also developed Nutrient Input Index (NI), assess whether geomorphology influences resource availability. found isotopic between analyzed. showed higher values wider niche ranges compared those reflecting more complex network tentatively associated its geological history. addition, relationship was observed proximity pools values, could suggest NI directly geomorphology, impacting selective forces across systems.

Language: Английский

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Optimised methods to image hepatic lipid droplets in zebrafish larvae

Disease Models & Mechanisms, Journal Year: 2024, Volume and Issue: 17(11)

Published: Oct. 7, 2024

ABSTRACT The optical transparency of zebrafish larvae enables visualization subcellular structures in intact organs, and these vertebrates are widely used to study lipid biology liver disease. Lipid droplet (LD) presence is a prevalent feature healthy cells, but, under conditions such as nutrient excess, toxicant exposure or metabolic imbalance, LD accumulation hepatocytes can be harbinger more severe forms We undertook comprehensive analysis approaches useful investigate distribution dynamics physiological pathological the larvae. This comparative dyes Oil Red O, Nile Red, LipidTox LipidSpot, well transgenic reporters that rely on EGFP fusions LD-decorating protein perilipin 2 (PLIN2), demonstrates strengths limitations each approach. These protocols amenable detection methods ranging from low-resolution stereomicroscopy confocal imaging, which measurements hepatic size, number at cellular resolution live fixed animals. resource will benefit investigators studying disease models.

Language: Английский

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Postprandial Sleep in Short‐Sleeping Mexican Cavefish

Journal of Experimental Zoology Part A Ecological and Integrative Physiology, Journal Year: 2024, Volume and Issue: 341(10), P. 1084 - 1096

Published: Nov. 13, 2024

ABSTRACT Interactions between sleep and feeding behaviors are critical for adaptive fitness. Diverse species suppress when food is scarce to increase the time spent foraging. Postprandial sleep, an in following a event, has been documented vertebrate invertebrate animals. While interactions appear be highly conserved, evolution of postprandial response changes availability remains poorly understood. Multiple populations Mexican cavefish, Astyanax mexicanus , have independently evolved loss increased consumption compared surface‐dwelling fish same species, providing opportunity investigate feeding. Here, we effects on larval adult surface fish, two parallelly cave A. . Larval immediately meal, first evidence model. The amount was not correlated meal size occurred time. In contrast larvae, detected or which can survive months without food. Together, these findings reveal that present multiple short‐sleeping suggesting sleep‐feeding retained despite loss. These raise possibility energy conservation survival larvae sensitive deprivation.

Language: Английский

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Postprandial sleep in short-sleeping Mexican cavefish

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 5, 2024

Abstract Interaction between sleep and feeding behaviors are critical for adaptive fitness. Diverse species suppress when food is scarce to increase the time spent foraging. Post-prandial sleep, an in following a event, has been documented vertebrate invertebrate animals. While interactions appear be highly conserved, evolution of postprandial response changes availability remains poorly understood. Multiple populations Mexican cavefish, Astyanax mexicanus, have independently evolved loss increased consumption compared surface-dwelling fish same species, providing opportunity investigate feeding. Here, we effects on larval adult surface fish, two parallelly cave A. mexicanus. Larval mexicanus immediately meal, first evidence model. The amount was not correlated meal size occurred time. In contrast larvae, detected or that can survive months without food. Together, these findings reveal present multiple short-sleeping suggesting sleep-feeding retained despite loss. These raise possibility energy conservation survival larvae sensitive deprivation.

Language: Английский

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Comparative analysis of the effects of dietary supplementation with Ampelopsis grossedentata extract and dihydromyricetin on growth performance, muscle quality, and gut microbiota of Megalobrama hoffmanni

Aquaculture, Journal Year: 2024, Volume and Issue: unknown, P. 742049 - 742049

Published: Dec. 1, 2024

Language: Английский

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