Self-Assembled Peptide-Gold Nanoparticle 1D Nanohybrids Functionalized with GHK Tripeptide for Enhanced Wound-Healing and Photothermal Therapy

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Glycyl-l-histidyl-l-lysine (GHK) tripeptides are known for their remarkable therapeutic potential, including wound-healing, anti-inflammatory activity, and cellular regeneration. However, clinical application has been significantly hindered by poor biological stability limited efficacy in a physiological medium. In this study, we introduce sophisticated approach to overcome these limitations developing supramolecular peptide nanofiber-gold (Au) nanoparticle (NP) hybrids functionalized with GHK tripeptides. By strategically manipulating self-assembly NP integration, demonstrated useful platform that enhances both material stability. Our methodology involves the precise engineering of 9-fluorenylmethoxycarbonyl-diphenylalanine scaffolds KHG tripeptides, enabling robust nanofibril formation through π–π stacking hydrogen bonding. Critically, discovered specific amino acid sequence influences surface exposure lysine, directly impacting nanohybrid's wound-healing capabilities. The resultant nanohybrids exhibit exceptional characteristics: Au NPs spatially confined within nanofibers, achieving remarkably uniform size distribution approximately 3 nm. These demonstrate superior near-infrared (NIR) light absorption photothermal conversion efficiency, effective eradication cancer cells organoids killing under NIR irradiation. This dual-functional nanohybrid integrates biocompatible enzymatically degradable achieve synergistic cancer-killing effects. mitigating cytotoxicity biodegradability issues associated conventional agents, our system provides promising strategy improve postoperative therapy promote tissue work highlights potential peptide-inorganic advancing multifunctional platforms treatment repair.

Language: Английский

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Green Synthesis of Gold Nanoparticles Using Clerodendrum trichotomum Thunberg for Antibacterial and Anticancer Applications

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 2645 - 2658

Published: March 1, 2025

The potential use of gold nanoparticles (AuNPs) in healthcare research has increased dramatically recent years, owing to advancements their synthesis techniques, including green synthesis. Although Clerodendrum trichotomum is a popular medicinal plant that harbors many bioactive phytochemicals various parts the world, AuNPs using this precious still under investigation. Therefore, study aimed explore from C. Thunberg leaves (CTT-AuNPs) and antibacterial cytotoxic properties. were synthesized characterized microscopic spectroscopic techniques. A serial dilution technique was used determine activity CTT-AuNPs against two bacterial species, Klebsiella pneumoniae Staphylococcus aureus. cytotoxicity assay carried out breast cancer cell line (MCF-7), whereas biocompatibility test performed kidney (293T). Multiple characterization techniques confirmed successful CTT-AuNPs. average size 19.1±2.2 nm. results revealed showed strong S. aureus K. pneumoniae, which further live/dead dual staining assay. also significantly reduced proliferation MCF-7 cells 32.67% at 120 µg/mL after 24 hours incubation. These green-synthesized exhibited excellent cytobiocompatibility with 293T cells. method compared chemically AuNPs. This work will pave way for production biocompatible can be different disease treatments.

Language: Английский

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BMP2 peptide-modified polycaprolactone-collagen nanosheets for periodontal tissue regeneration

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 5, 2025

Periodontitis leads to the degradation of tooth-supporting tissues, ultimately causing tooth mobility and loss. Guided tissue regeneration (GTR) surgery employs barrier membranes facilitate regeneration. However, conventional lack bone-inducing properties, thereby limiting their efficacy. Our objective was develop a bifunctional GTR membrane that combines mechanical stability with capabilities. To achieve this, we engineered BMP2 peptide-modified polycaprolactone-collagen nanosheets (BPCNs) enhance periodontal by improving cell adhesion, osteogenesis, anti-inflammatory activity. BPCNs nanoscale thickness were fabricated using spin-coating technique, incorporating peptides, collagen, polycaprolactone (PCL), polyvinyl alcohol (PVA). Successful conjugation verified through UV spectrophotometry confocal laser scanning microscopy. The biocompatibility adhesion properties rigorously assessed CCK-8 assays, microscopic imaging, quantitative counting. In vitro osteogenic efficacy evaluated Alizarin Red S (ARS) staining reverse transcription polymerase chain reaction (qRT-PCR) analyze marker gene expression. A rat defect model established assess in vivo regenerative performance, outcomes analyzed micro-CT, hematoxylin-eosin (H&E) staining, Masson's trichrome confirming enhanced absence systemic toxicity. mechanistic pathways underlying BPCNs-mediated elucidated via RNA sequencing (RNA-seq), revealing activation signaling cascades suppression proinflammatory pathways. demonstrated excellent biocompatibility, promoted fibroblast bone marrow stem (BMSC) BMSC osteogenesis. Furthermore, significantly model. Mechanistically, RNA-seq analysis revealed upregulated genes involved downregulated This study introduced novel osteoinductive nanosheet, termed BPCNs, which provides groundbreaking material-based approach for repair defects. These findings position as highly promising candidate surgery, significant potential improve clinical medicine.

Language: Английский

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Exploration of plant alkaloids as potential inhibitors of HIV–CD4 binding: Insight into comprehensive in silico approaches

Open Chemistry, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 1, 2025

Abstract This study investigates the potential of alkaloids – nitidine, harmine, harmaline, berberine, and magnoflurine as inhibitors HIV –CD4 binding, focusing on their molecular interactions, binding affinities, pharmacokinetic properties. Molecular docking results revealed that all exhibited high-affinity to CD4 receptor showed significant interactions with spike protein ( -SP). Interaction analysis nitidine harmaline formed hydrogen bonds hydrophobic while harmine relied van der Waals forces π-stacking. Pharmacokinetic evaluations, based Lipinski’s Rule Five, favorable oral bioavailability, moderate lipophilicity, blood–brain barrier permeability, having a bioavailability score 0.55, showing promising candidate for development anti- therapeutics, supported by solid stability in MD simulations, profiles. simulations confirmed CD4– -SP complex, magnesium fluoride highest strength minimal structural deviation, berberine stability. MM-GBSA calculations most potent inhibitor free energy.

Language: Английский

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Right Once for All: Inflammation‐Targeted Zinc–Cobalt Metal–Organic Framework Nanozymes with High Stability and Broad‐Spectrum Synergistic Antibacterial Properties for Accelerating Bacteria‐Infected Wound Healing under Physiological Conditions

Small Structures, Journal Year: 2025, Volume and Issue: unknown

Published: April 6, 2025

Zinc‐modified cobalt‐based metal–organic frameworks (ZCM) can effectively generate abundant reactive oxygen species to heal bacterially infected wounds. However, their instability under physiological conditions not only weakens its catalytic function but also leads the accumulation of large amounts metal ions, thereby increasing possibility new wound infections. In this study, synergistic antibacterial nanoparticles (ZCMCC) are synthesized by loading chlorogenic acid (CGA) on ZCM with chitosan modification increase presence carboxyl groups in structure and enhance stability. Meanwhile, ZCMCC exerted broad‐spectrum activities against Gram‐negative bacteria ( Escherichia coli, 98%), Gram‐positive Staphylococcus aureus , 99.4%), methicillin‐resistant S. (99.3%) chemodynamic therapy release CGA. Importantly, promotes healing wounds expression vascular endothelial growth factor α targets infective areas combining CD44. Hence, research presents a novel approach for creating system developing targeted therapies deep tissue/organ infections future.

Language: Английский

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Multifunctional Dual-Layer Microneedles Loaded with Selenium-Doped Carbon Quantum Dots and Astilbin for Ameliorating Diabetic Wound Healing

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101739 - 101739

Published: April 1, 2025

Diabetic wounds (DW) represent a significant clinical challenge due to chronic inflammation, excessive oxidative stress, and impaired angiogenesis, all of which hinder effective tissue regeneration. Existing drug delivery systems often fail achieve sustained targeted therapeutic efficacy. In this study, we developed novel dissolvable dual-layer methacrylated gelatin (GelMA) microneedle (MN) co-loading selenium-doped carbon quantum dots (Se-CQDs) Astilbin (AST) for enhanced DW treatment. The outer layer, enriched with Se-CQDs, rapidly scavenges reactive oxygen species (ROS), effectively alleviating stress at the wound site. Sequentially, inner layer releases AST, exerting potent anti-inflammatory pro-angiogenic effects. Preliminary findings suggest these effects may involve modulation cytoskeletal dynamics peroxisome function, contributing endothelial cell migration angiogenesis. This controlled, sequential release MN establishes low-oxidative, microenvironment, thereby promoting angiogenesis accelerating repair. pioneering integration AST-loaded hydrogels offers synergistic strategy, setting new standard advanced diabetic care substantial promise.

Language: Английский

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Current Status and Perspectives of Research on Polymer Hydrogels in the Treatment and Protection of Osteoarthritis

Macromolecular Bioscience, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Arthritis is a degenerative disease characterized by chronic cartilage degeneration. It affects hundreds of millions people worldwide and often has serious consequences such as joint pain swelling, limited mobility, deformity. However, conventional treatments still struggle to achieve satisfactory results. Finding more effective for arthritis remains an important clinical challenge. As hydrogels have unique 3D spatial mesh structure, significant material interaction ability, adjustable mechanical properties, good biodegradability, they can provide suitable cellular or tissue microenvironment, their potential in scaffolding effect, lubrication, anti‐inflammatory drug delivery expected be potent therapeutic approach the treatment osteoarthritis. In this review, three aspects hydrogel products osteoarthritis are comprehensively summarized discussed, namely, selection gel design, exploration cross‐linking mechanisms, mechanisms therapy osteoarthritis, focus on advantages limitations applications, which point out direction development strategy innovative field, applied research, transformation.

Language: Английский

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Magnesium Ion/Gallic Acid MOF-Laden Multifunctional Acellular Matrix Hydrogels for Diabetic Wound Healing

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 3, 2025

The main objective for diabetic wound treatment is the design of a functional dressing that scavenges free radicals, alleviates inflammation, and antibacterial while promoting neovascularization. Herein, multifunctional acellular matrix hydrogel was prepared with antimicrobial peptide jelleine-1 magnesium ion/gallic acid metal framework to exhibit antioxidant, anti-inflammatory, proangiogenesis effects in wounds. termed Gel-J-MOF efficiently released gallic acidic microenvironment wound, scavenged excess radicals vitro, effectively reduced levels inflammation by regulating M2 macrophage polarization vivo. composite inhibited S. aureus E. coli suitable healing. In later stage healing, stimulated angiogenesis, accelerating re-epithelialization collagen deposition wound. conclusion, this provides regulated treating wounds and, therefore, has significant potential application promise chronic

Language: Английский

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Core-shell nanofiber dressings with zinc oxide nanoparticles and cell-free fat extract: boosting fibroblast activity and antibacterial efficacy

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: May 19, 2025

This study presents the development and characterization of innovative core-shell nanofiber wound dressings incorporating zinc oxide nanoparticles (nZnO) cell-free fat extract (CEFFE) to enhance fibroblast activity antibacterial efficacy. CEFFE was prepared analyzed, revealing high concentrations essential growth factors, particularly bFGF TGF-β1, supporting its therapeutic potential in tissue regeneration. The fabricated nanofibers (PLCL, nZnO/PLCL, PLCL-CEFFE/HA, nZnO/PLCL-CEFFE/HA) were examined using FE-SEM TEM, demonstrating successful encapsulation morphological variations due nZnO incorporation. XRD analysis confirmed structural integrity effective loading CEFFE. Hydrophilicity assessment via water contact angle measurements showed that CEFFE/HA significantly enhanced hydrophilicity PLCL membranes, crucial for exudate management. Mechanical tests indicated addition maintained scaffold's mechanical robustness, while slightly reduced properties. In vitro release studies revealed a biphasic pattern Zn²⁺ ions factors from nZnO/PLCL-CEFFE/HA nanofibers, ensuring prolonged sustained effects. Antibacterial assays demonstrated significant efficacy against E. coli S. aureus, attributed nZnO. MTT NIH-3T3 cell proliferation adhesion on PLCL-CEFFE/HA controlled factors. scratch assay superior migration healing formulations. These findings underscore as multifunctional dressings, combining properties with regeneration capabilities. However, further are needed assess long-term stability vivo performance, which represent key challenges future research.

Language: Английский

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Multifunctional nitrogen-sulfur codoped carbon quantum dots: Determining reduced glutathione, broad-spectrum antibacterial activity, and cell imaging

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38177 - e38177

Published: Sept. 20, 2024

In this study, nitrogen-sulfur codoped carbon quantum dots (N-S/CQDs) with various functions and properties were synthesized through a one-step method utilizing citric acid cysteine as reaction substrates. The fluorescence of N-S/CQDs can be specifically quenched by permanganate ion (MnO

Language: Английский

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