EFSA Journal,
Journal Year:
2024,
Volume and Issue:
22(11)
Published: Nov. 1, 2024
Abstract
Genetically
modified
Komagataella
phaffii
strain
MXY0541
was
developed
to
produce
soy
leghemoglobin
by
introducing
the
LGB2
coding
sequence
encoding
from
soybean
(Glycine
max).
The
molecular
characterisation
data
and
bioinformatic
analyses
do
not
raise
any
safety
concerns.
of
as
a
food
additive
has
already
been
assessed
EFSA
FAF
Panel
(EFSA‐Q‐2022‐00031).
GMO
does
identify
concerns
regarding
toxicity
allergenicity
protein
expressed
in
K.
phaffii,
finds
no
evidence
that
genetic
modification
would
change
its
overall
allergenicity.
concludes
LegH
Prep
derived
genetically
is
safe
for
human
consumption
with
regard
effects
modification.
No
environmental
impact
use
this
product
expected
recombinant
DNA
sequences
possibly
remaining
product.
respect
potential
on
health
environment
at
proposed
level
far
concerned.
conclusion
lead
issues.
EFSA Journal,
Journal Year:
2025,
Volume and Issue:
23(4)
Published: April 1, 2025
Abstract
Following
a
request
from
the
European
Commission,
Panel
on
Additives
and
Products
or
Substances
used
in
Animal
Feed
(FEEDAP)
was
asked
to
deliver
scientific
opinion
l‐lysine
sulfate
produced
by
fermentation
with
non‐genetically
modified
strain
of
Corynebacterium
glutamicum
(CGMCC
23982)
when
as
nutritional
additive
feed
for
all
animal
species.
The
active
substance
is
l‐lysine.
FEEDAP
concluded
that
production
qualifies
qualified
presumption
safety
(QPS)
approach
assessment;
therefore,
C.
CGMCC
23982
does
not
pose
any
concern
associated
strain.
l‐Lysine
considered
safe
target
species
administered
via
feed.
When
using
sulfate,
background
sulfur/sulfate
content
compound
should
be
taken
into
account
formulating
diets.
has
concerns
use
water
drinking.
consumer
environment.
With
regard
user
safety,
irritant
skin,
eyes
respiratory
tract.
Any
exposure
risk.
an
efficacious
source
essential
amino
acid
non‐ruminant
For
supplemental
ruminants
species,
it
would
require
protection
against
degradation
rumen.
EFSA Journal,
Journal Year:
2024,
Volume and Issue:
22(7)
Published: July 1, 2024
Abstract
EFSA
was
requested
by
the
European
Commission
(in
accordance
with
Article
29
of
Regulation
(EC)
No
178/2002)
to
provide
a
scientific
opinion
on
application
new
developments
in
biotechnology
(new
genomic
techniques,
NGTs)
viable
microorganisms
and
products
category
4
be
released
into
environment
or
placed
market
as
food
feed,
non‐viable
3
feed.
A
horizon
scanning
exercise
identified
variety
containing
obtained
NGTs
(NGT‐Ms),
falling
within
remit
EFSA,
that
are
expected
(EU)
next
10
years.
novel
potential
hazards/risks
from
NGT‐Ms
were
compared
those
established
techniques
(EGTs),
conventional
mutagenesis.
Due
higher
efficiency,
specificity
predictability
NGTs,
hazards
related
changes
genome
likely
less
frequent
than
modified
EGTs
It
is
concluded
guidances
‘partially
applicable’,
therefore
case‐by‐case
basis
for
specific
NGT‐Ms,
fewer
requirements
may
needed.
Some
‘not
sufficient’
updates
recommended.
Because
possible
relate
genotypic
phenotypic
introduced
not
method
used
modification,
it
recommended
any
guidance
should
take
consistent
risk
assessment
approach
strains/products
derived
produced
mutagenesis,
NGTs.
International Journal of Food Microbiology,
Journal Year:
2024,
Volume and Issue:
426, P. 110922 - 110922
Published: Sept. 26, 2024
Lactiplantibacillus
plantarum
(formerly
Lactobacillus
plantarum)
exhibits
relevant
probiotic
and
technological
features
is
widely
used
in
food
industries,
improving
flavour,
texture
organoleptic
properties
of
fermented
products.
Cell-surface
proteins
have
a
key
role
the
molecular
mechanisms
responsible
for
healthy
effects,
being
first
actors
bacteria
-
host
interactions.
Proteins
present
on
surface
four
L.
strains
(two
isolated
from
vegetable
matrices
two
dairy
products)
were
identified
by
proteomics
with
aim
to
gain
comprehensive
picture
differences
protein
profiles
potentially
related
habitat
origin
specific
analyzed
strains.
Results
highlighted
more
diversified
pattern
compared
those
(>500
vs
about
200
proteins,
respectively).
The
shared
core
143
while
445
specifically
26
peculiar
origin.
Sortase
A,
involved
adhesion,
choloylglycine
hydrolase
(bile
salt
hydrolase)
detected
only
matrices.
functions
suggested
that
these
strains,
particular
S61,
could
significant
biotechnological
potential.
ScienceAsia,
Journal Year:
2024,
Volume and Issue:
50(2), P. 1 - 1
Published: Jan. 1, 2024
This
study
aimed
to
explore
bacteriocin-producing
lactic
acid
bacteria
(LAB),
identify
the
genes
influencing
bacteriocin
synthesis,
and
assess
their
probiotic
potential
via
safety
evaluations
both
in
vitro
silico.The
strain
ZBK1-5,
isolated
from
pickled
ginger,
was
identified
as
Lactiplantibacillus
plantarum.The
ZBK1-5
exhibited
highest
antimicrobial
activity
with
6,400
AU/ml
at
18
h,
predominantly
effective
against
Gram-positive
stable
within
pH
2-9.Significantly,
genome
analysis
confirmed
existence
of
synthesis
genes,
specifically
plantaricin
KJ,
EF,
A,
N.
Simultaneously,
showed
its
low
risk
antibiotic
resistance
gene
transfer,
thereby
emphasizing
profile
ZBK1-5.The
genetic
components
associated
ability
L.
plantarum
survive
adapt
gastrointestinal
conditions
provide
additional
evidence
(16
related
stress
9
bile
resistance),
supporting
a
candidate.These
findings
were
validated
through
digestion
conditions,
where
viability
cells
observed
be
68.26,66.62,
60.91%
during
transit
oral,
gastrointestinal,
small
intestinal
phases,
respectively.Additionally,
90.39%
adhesion
rate
Caco-2
cell
line.The
unique
characteristics
produced
by
exhibit
potent
antibacterial
activity,
rapid
time
production
compared
other
known
plantaricins,
for
application
upscaling.This
research
offers
significant
scientific
insights
into
producer,
promising
future
applications.
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(8), P. 1652 - 1652
Published: Aug. 12, 2024
The
traditional
Mexican
fermented
beverage
pulque
has
been
considered
a
healthy
product
for
treating
gastrointestinal
disorders.
Lactic
acid
bacteria
(LAB)
have
identified
as
one
of
the
most
abundant
microbial
groups
during
fermentation.
As
is
consumed
directly
from
fermentation
vessel,
naturally
associated
LABs
are
ingested,
reaching
consumer’s
small
intestine
alive,
suggesting
their
potential
probiotic
capability.
In
this
contribution,
we
assayed
strain
Lactiplantibacillus
plantarum
LB1_P46
isolated
produced
in
Huitzilac,
Morelos
State,
Mexico.
characterization
included
resistance
to
pH
(3.5)
and
exposure
bile
salts
at
37
°C;
assay
hemolytic
activity
antibiotic
profiling;
functional
traits
cholesterol
reduction
β-galactosidase
activity;
several
cell
surface
properties,
indicating
that
LAB
possesses
properties
comparable
other
LAB.
Additionally,
L.
showed
significance
vitro
antimicrobial
against
Gram-negative
Gram-positive
vivo
preventive
anti-infective
capability
Salmonella
BALB/c
mouse
model.
Several
activities
were
correlated
with
corresponding
enzymes
encoded
complete
genome
strain.
mining
bacteriocins
led
identification
ribosomally
synthesized
post-translationally
modified
peptide
encoding
plantaricin
EF.
Results
indicated
promising
preparing
non-dairy
dairy
beverages.
EFSA Journal,
Journal Year:
2024,
Volume and Issue:
22(8)
Published: Aug. 1, 2024
Abstract
The
conclusions
of
the
European
Food
Safety
Authority
(EFSA)
following
peer
review
initial
risk
assessments
carried
out
by
competent
authority
rapporteur
Member
State,
Netherlands
for
pesticide
active
substance
Phthorimaea
operculella
granulovirus
are
reported.
context
was
that
required
Regulation
(EC)
No
1107/2009
Parliament
and
Council.
were
reached
on
basis
evaluation
representative
uses
as
an
insecticide
tomato
(field
greenhouse
uses)
potato
use)
via
spraying
(tractor
drawn
or
knapsack
sprayers)
overhead
irrigation.
reliable
endpoints,
appropriate
use
in
regulatory
assessment
presented.
Missing
information
identified
being
framework
is
listed.
