Isolation, identification and biological characterization of bacterial endophytes isolated from Gunnera Perpensa L. DOI Creative Commons
Siphiwe G. Mahlangu, Nodumo Nokulunga Zulu, Mahloro Hope Serepa‐Dlamini

et al.

FEMS Microbiology Letters, Journal Year: 2024, Volume and Issue: 371

Published: Jan. 1, 2024

Abstract In the present study, eleven endophytic bacterial strains, Herbaspirillum sp. (GP-SGM1, GP-SGM2, GP-SGM3, and GP-SGM11), Pseudomonas (GP-SGM4, GP-SGM5), Novosphingobium GP-SGM6, Chryseobacterium GP-SGM7, Labedella GP-SGM8, Brevibacterium GP-SGM9, GP-SGM10, were isolated from rhizomes of Gunnera perpensa L. The growth kinetics, assessed through maximum rates (μmax) optical density (OD) values, revealed that GP-SGM7 exhibited highest μmax values 0.33 ± 0.01 hours (h)−1 with an OD 4.20 0.04. contrast, GP-SGM11 lowest 0.12 0.05 h−1 smallest 1.50 0.00. addition, endophyte crude extracts tested for antibacterial activity against five pathogenic strains using disk diffusion method, exhibiting promising Klebsiella pneumoniae Staphylococcus aureus. Antioxidant was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl) FRAP (ferric reducing antioxidant power) assays. GP-SGM1, GP-SGM10 most effective at scavenging radicals, also a high value 0.54 0.01. These findings emphasize therapeutic potential bacteria G. in addressing skin-related issues, including infections free radicals.

Language: Английский

Isolation, identification and biological characterization of bacterial endophytes isolated from Gunnera Perpensa L. DOI Creative Commons
Siphiwe G. Mahlangu, Nodumo Nokulunga Zulu, Mahloro Hope Serepa‐Dlamini

et al.

FEMS Microbiology Letters, Journal Year: 2024, Volume and Issue: 371

Published: Jan. 1, 2024

Abstract In the present study, eleven endophytic bacterial strains, Herbaspirillum sp. (GP-SGM1, GP-SGM2, GP-SGM3, and GP-SGM11), Pseudomonas (GP-SGM4, GP-SGM5), Novosphingobium GP-SGM6, Chryseobacterium GP-SGM7, Labedella GP-SGM8, Brevibacterium GP-SGM9, GP-SGM10, were isolated from rhizomes of Gunnera perpensa L. The growth kinetics, assessed through maximum rates (μmax) optical density (OD) values, revealed that GP-SGM7 exhibited highest μmax values 0.33 ± 0.01 hours (h)−1 with an OD 4.20 0.04. contrast, GP-SGM11 lowest 0.12 0.05 h−1 smallest 1.50 0.00. addition, endophyte crude extracts tested for antibacterial activity against five pathogenic strains using disk diffusion method, exhibiting promising Klebsiella pneumoniae Staphylococcus aureus. Antioxidant was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl) FRAP (ferric reducing antioxidant power) assays. GP-SGM1, GP-SGM10 most effective at scavenging radicals, also a high value 0.54 0.01. These findings emphasize therapeutic potential bacteria G. in addressing skin-related issues, including infections free radicals.

Language: Английский

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