LncRNA GAS5 restrains ISO-induced cardiac fibrosis by targeting miR-217 through regulating SIRT1 DOI Creative Commons
Yanhong Zhang,

Tingting Sun,

Zhenhuan Liu

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 18, 2023

Abstract Aims : Considering the SIRT1 has effect of improving myocardial fibrosis and GAS5 can inhibit occurrence development at cellular level, aim present study was to investigate whether LncRNA could attenuate cardiac through regulating miR-217/SIRT1, NLRP3 inflammasome activation involved in this process. Methods Results: Isoprenaline (ISO) given subcutaneously male C57BL/6 mice induce AAV9 vectors were randomly injected into left ventricle each mouse overexpress long-chain non-coding RNA GAS5. Primary fibroblasts (MCFs) derived from neonatal TGF-β1 used fibrosis. And overexpressed MCFs treated with miR-217 mimics inhibitor respectively. Then assays expression levels NLRP3, Caspase-1, IL-1βand conducted. The Results showed that overexpression reduced collagen, Capase-1, IL-1β ISO MCFs. However, significantly weakened after overexpression, but further enhanced knockdown miR-217. Conclusion: down-regulats SIRT1, then aggravates activation-mediated pyroptosis. alleviates induced by pyroptosis via miR-217/SIRT1 pathway. This provides significant experimental evidence suggesting be a potential therapeutic target for other fibrotic diseases.

Language: Английский

The role of the inflammasome and pyroptosis in heart failure DOI Creative Commons
Jin-yi Li,

Y L Ye,

Yingjie Yang

et al.

European Journal of Inflammation, Journal Year: 2023, Volume and Issue: 21

Published: June 1, 2023

Heart failure (HF) is a complicated clinical syndrome caused by abnormal cardiac structure and/or function, leading to ventricular filling ejection dysfunction. Pyroptosis newly identified form of programmed cell death often accompanied inflammation during HF. The NLRP3 inflammasome, acting as caspase-1 activation platform, plays critical role in the production key pro-inflammatory cytokines and pyroptosis. Therefore, inflammasomes are involved pathophysiological process Our paper will review latest research progress on relationship among pyroptosis, inflammasomes,

Language: Английский

Citations

1
LncRNA GAS5 restrains ISO-induced cardiac fibrosis by targeting miR-217 through regulating SIRT1 DOI Creative Commons
Yanhong Zhang,

Tingting Sun,

Zhenhuan Liu

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 18, 2023

Abstract Aims : Considering the SIRT1 has effect of improving myocardial fibrosis and GAS5 can inhibit occurrence development at cellular level, aim present study was to investigate whether LncRNA could attenuate cardiac through regulating miR-217/SIRT1, NLRP3 inflammasome activation involved in this process. Methods Results: Isoprenaline (ISO) given subcutaneously male C57BL/6 mice induce AAV9 vectors were randomly injected into left ventricle each mouse overexpress long-chain non-coding RNA GAS5. Primary fibroblasts (MCFs) derived from neonatal TGF-β1 used fibrosis. And overexpressed MCFs treated with miR-217 mimics inhibitor respectively. Then assays expression levels NLRP3, Caspase-1, IL-1βand conducted. The Results showed that overexpression reduced collagen, Capase-1, IL-1β ISO MCFs. However, significantly weakened after overexpression, but further enhanced knockdown miR-217. Conclusion: down-regulats SIRT1, then aggravates activation-mediated pyroptosis. alleviates induced by pyroptosis via miR-217/SIRT1 pathway. This provides significant experimental evidence suggesting be a potential therapeutic target for other fibrotic diseases.

Language: Английский

Citations

0