The role of GZMA as a target of cysteine and biomarker in Alzheimer’s disease, pelvic organ prolapse, and tumor progression DOI Creative Commons
Yan Li,

Zhuo Wang,

Min Gyu Kong

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 20, 2024

Objective: This study aims to investigate how changes in peripheral blood metabolites Alzheimer’s Disease (AD) patients affect the development of Pelvic Organ Prolapse (POP) using a multi-omics approach. We specifically explore interactions signaling pathways, gene expression, and protein-metabolite interactions, with focus on GZMA cysteine age-related diseases. Methods: utilized analysis, including metabolomics transcriptomics, evaluate perturbations their effect POP AD patients. Additionally, comprehensive pan-cancer immune infiltration analysis was performed core targets combined POP, exploring potential roles tumor progression elucidating pharmacological relevance solid tumors. Results: identified 47 differential linked 9 significant such as unsaturated fatty acid biosynthesis amino metabolism. A thorough expression revealed numerous differentially expressed genes (DEGs), Gene Set Enrichment Analysis (GSEA) showing profiles POP. Network topology highlighted central nodes AD-POP co-expressed network. Functional analyses indicated involvement critical biological processes pathways. Molecular docking studies showed strong between proteins PTGS2 GZMA, molecular dynamics simulations confirmed stability these complexes. In vitro validation demonstrated that reduced ROS levels protected cell viability. widely various cancers, associated cells, correlated patient survival prognosis. Conclusion: Multi-omics role biomarkers therapeutic targets, emphasizing effectiveness integrative approaches treating concurrently. The findings highlight need for in-depth research novel advance strategies.

Language: Английский

Role of MLIP in burn-induced sepsis and insights into sepsis-associated cancer progression DOI Creative Commons
Zhiwei Li, Qian Wang, Ying Liu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 14, 2025

Introduction Burn-induced sepsis is a critical clinical challenge marked by systemic inflammation, immune dysregulation, and high mortality. Macrophage-driven inflammatory pathways are central to pathogenesis, while cell metabolic reprogramming plays key role in both cancer progression. Methods Bioinformatics analyses using GEO, TCGA, GTEx datasets identified MLIP-modulated genes linked responses prognosis. In vitro , LPS-stimulated HUVEC cells were used study MLIP’s effects on inflammation macrophage function through viability, ROS levels, cytokine expression, qRT-PCR, immunofluorescence assays. Results associated with immune-related cancer. Epigenetic analysis showed MLIP expression regulated promoter methylation chromatin accessibility. Prognostic revealed impact survival outcomes across types. reduced oxidative stress, hyperactivation. Conclusions regulates immune-metabolic dynamics burn-induced sepsis, influencing activity stress. Its suggests as potential therapeutic target linking modulation Further research evasion tumor metabolism may inform novel strategies.

Language: Английский

Citations

0
Regulatory role of CD177+ neutrophils in BMP signaling pathway and its implications for inflammatory bowel disease, sepsis, and intestinal tumors DOI
Lin Yu,

Haoyue Zhang,

Ling Jia

et al.

Translational Oncology, Journal Year: 2025, Volume and Issue: 55, P. 102336 - 102336

Published: April 1, 2025

Language: Английский

Citations

0
Association between polymorphisms of the adenylate cyclase 3 gene rs2241759 and the effect of high-intensity interval training on blood lipid profiles DOI Creative Commons

Junren Lai,

Li Gong, Yan Liu

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19271 - e19271

Published: April 11, 2025

One of the recognized effects systematic physical activity is improvement fitness, with a negative correlation found between fitness and cardiovascular cardiometabolic risk. The purpose this study to analyze influence single nucleotide polymorphisms (SNPs) adenylate cyclase 3 (ADCY3) gene on effect high-intensity interval training (HIIT) blood lipids, simultaneously screen out genetic markers sensitive HIIT in Chinese Han youth. In 12-week program, total 237 college students non-regular exercise habits were recruited, these volunteers participated three times week. Baseline after cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C) low-density (LDL-C) measured, respectively. DNA was extracted from white cells genotyping carried out. PLINK v1.09 software used conduct quality control screening obtained SNPs, linear regression model constructed association ADCY3 SNPs lipids. ANOVA multiple comparison (LSD) performed test difference groups (P < 0.05). (1) Through analysis Illumina CGA chip scanning, 22 identified. Following rigorous screening, 15 included subsequent analysis. Notably, it that rs2241759 locus associated lipid profiles. (2) Among male volunteers, significant differences baseline HDL-C values observed among genotypes at (β = - 0.019, P 0.040). value for GG genotype higher than AA/AG genotypes. (3) After HIIT, levels TC increased significantly contrast, TG LDL-C decreased Further statistical categorized by gender revealed that, exception men, changes TC, TG, HDL-C, across both genders (4) Compared genotype, carrying A allele exhibited more pronounced change following 0.044, 0.038). (5) demonstrated 0.065, 0.04363). implementation regimen can enhance status students. sensitivity HIIT.

Language: Английский

Citations

0
Functions of Coenzyme A and Acyl-CoA in Post-Translational Modification and Human Disease DOI Creative Commons
Jumin Xie, Yu Zhang, Ying Zhu

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(9)

Published: Sept. 24, 2024

Coenzyme A (CoA) is synthesized from pantothenate, L-cysteine and adenosine triphosphate (ATP), plays a vital role in diverse physiological processes. Protein acylation common post-translational modification (PTM) that modifies protein structure, function interactions. It occurs via the transfer of acyl groups acyl-CoAs to various amino acids by acyltransferase. The characteristics effects vary according origin, location group. Acetyl-CoA, formyl-CoA, lactoyl-CoA, malonyl-CoA are typical group donors. major donor, acyl-CoA, enables modifications impart distinct biological functions both histone non-histone proteins. These crucial for regulating gene expression, organizing chromatin, managing metabolism, modulating immune response. Moreover, CoA acyl-CoA play significant roles development progression neurodegenerative diseases, cancer, cardiovascular other health conditions. goal this review was systematically describe types commonly utilized acyl-CoAs, their PTM, human diseases.

Language: Английский

Citations

1
The role of GZMA as a target of cysteine and biomarker in Alzheimer’s disease, pelvic organ prolapse, and tumor progression DOI Creative Commons
Yan Li,

Zhuo Wang,

Min Gyu Kong

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 20, 2024

Objective: This study aims to investigate how changes in peripheral blood metabolites Alzheimer’s Disease (AD) patients affect the development of Pelvic Organ Prolapse (POP) using a multi-omics approach. We specifically explore interactions signaling pathways, gene expression, and protein-metabolite interactions, with focus on GZMA cysteine age-related diseases. Methods: utilized analysis, including metabolomics transcriptomics, evaluate perturbations their effect POP AD patients. Additionally, comprehensive pan-cancer immune infiltration analysis was performed core targets combined POP, exploring potential roles tumor progression elucidating pharmacological relevance solid tumors. Results: identified 47 differential linked 9 significant such as unsaturated fatty acid biosynthesis amino metabolism. A thorough expression revealed numerous differentially expressed genes (DEGs), Gene Set Enrichment Analysis (GSEA) showing profiles POP. Network topology highlighted central nodes AD-POP co-expressed network. Functional analyses indicated involvement critical biological processes pathways. Molecular docking studies showed strong between proteins PTGS2 GZMA, molecular dynamics simulations confirmed stability these complexes. In vitro validation demonstrated that reduced ROS levels protected cell viability. widely various cancers, associated cells, correlated patient survival prognosis. Conclusion: Multi-omics role biomarkers therapeutic targets, emphasizing effectiveness integrative approaches treating concurrently. The findings highlight need for in-depth research novel advance strategies.

Language: Английский

Citations

0