Activation of GPER1 alleviates white matter injury by promoting microglia M2 polarization through EGFR/Stat3 pathway in intracerebral hemorrhage mice

Journal of Stroke and Cerebrovascular Diseases, Journal Year: 2025, Volume and Issue: 34(6), P. 108315 - 108315

Published: April 12, 2025

White matter injury (WMI) is a major pathophysiological process after intracerebral hemorrhage (ICH). G protein-coupled estrogen receptor 1 (GPER1) has been validated to exert crucial role in regulating neuroinflammation and microglia polarization. Our previous report reveals activation of GPER1 improves the neurological deficits ICH via inhibition A1 astrocytes. However, on protection WMI modulation polarization remains unclear. In present study, mice model was induced by autologous whole blood injection vitro established treatment BV2 cells with FeSO4. Mice were treated agonist G1, antagonist G15 or EGFR inhibitor AG1478. Besides, conditional medium used intervene MO3.13 oligodendrocytes. Immunostaining, immunoblots, transmission electron microscope PI staining determine WMI, potential molecular mechanism ICH, respectively. data showed G1 ameliorated day 3 ICH. reduced release IL-1β, TNF-α increased produce IL-4, IL-10 as well shifting from proinflammatory M1 anti-inflammatory M2 phenotype vivo vitro. Meanwhile, alleviated oligodendrocytes death mitigating modulating Mechanistic study demonstrated EGFR/Stat3 signaling pathway involved Collectively, our findings through pathway.

Language: Английский

Lupeol mitigates spinal cord injury by modulating microglial M1/M2 polarization via Na+/K+-ATPase-mediated mitophagy

Cellular Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 104955 - 104955

Published: April 1, 2025

Language: Английский