FEBS Open Bio,
Journal Year:
2024,
Volume and Issue:
14(11), P. 1805 - 1824
Published: Oct. 8, 2024
Mushrooms
are
the
fruiting
bodies
of
fungi
and
important
reproductive
structures
that
produce
disseminate
spores.
The
Pri3
gene
was
originally
reported
to
be
specifically
expressed
in
primordia
(a
precursor
mature
body)
edible
mushroom
Cyclocybe
aegerita
.
Here,
we
cloned
a
‐related
cDNA
from
cylindracea
,
another
species
same
genus,
showed
is
at
pileus
surface
immature
body
but
not
primordia.
Immunohistochemistry
translated
protein
secreted
into
polysaccharide
layer
surface.
recombinant
C
‐terminal
Cys‐rich
domain
antifungal
activity
against
three
filamentous
inhibited
hyphal
growth
conidiogenesis.
These
results
suggest
PRI3‐related
C.
named
cylindracin,
plays
an
role
defense
pathogens.
Nature Ecology & Evolution,
Journal Year:
2023,
Volume and Issue:
7(8), P. 1221 - 1231
Published: June 22, 2023
Fungi
are
ecologically
important
heterotrophs
that
have
radiated
into
most
niches
on
Earth
and
fulfil
key
ecological
services.
Despite
intense
interest
in
their
origins,
major
genomic
trends
of
evolutionary
route
from
a
unicellular
opisthokont
ancestor
to
derived
multicellular
fungi
remain
poorly
known.
Here
we
provide
highly
resolved
genome-wide
catalogue
gene
family
changes
across
fungal
evolution
inferred
the
genomes
123
relatives.
We
show
dominant
trend
early
has
been
gradual
shedding
protist
genes
punctuated
emergence
innovation
by
two
main
duplication
events.
find
content
non-Dikarya
resembles
opisthokonts
many
respects,
owing
conservation
genomes.
The
rapidly
duplicating
groups
included
extracellular
proteins
transcription
factors,
as
well
ones
linked
coordination
nutrient
uptake
with
growth,
highlighting
transition
sessile
osmotrophic
feeding
strategy
subsequent
lifestyle
elements
history.
These
results
suggest
pre-fungal
ancestors
evolved
typical
filamentous
genome
combination
loss,
turnover
several
large
events
rather
than
abrupt
changes.
Consequently,
taxonomically
defined
represents
genomically
non-uniform
assemblage
species.
Applied Microbiology and Biotechnology,
Journal Year:
2024,
Volume and Issue:
108(1)
Published: Feb. 19, 2024
Pleurotus
ostreatus,
also
known
as
the
oyster
mushroom,
is
a
popular
edible
mushroom
cultivated
worldwide.
This
review
aims
to
survey
recent
progress
in
molecular
genetics
of
this
fungus
and
demonstrate
its
potential
model
for
future
research.
The
development
modern
genetic
techniques
genome
sequencing
technologies
has
resulted
breakthroughs
science.
With
efficient
transformation
protocols
multiple
selection
markers,
powerful
toolbox,
including
such
gene
knockout
editing,
been
developed,
numerous
new
findings
are
accumulating
P.
ostreatus.
These
include
mechanisms
wood
component
degradation,
sexual
development,
protein
secretion
systems,
cell
wall
structure.
Furthermore,
these
enable
identification
horizons
enzymology,
biochemistry,
biology,
material
science
through
engineering,
fluorescence
microscopy,
breeding.
KEY
POINTS:
•
Various
available
ostreatus
can
be
used
an
alternative
analyses.
New
frontiers
being
developed
using
fungus.
Current Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Lichens
are
composite,
symbiotic
associations
of
fungi,
algae,
and
bacteria
that
result
in
large,
anatomically
complex
organisms
adapted
to
many
the
world's
most
challenging
environments.
How
such
intricate,
self-replicating
lichen
architectures
develop
from
simple
microbial
components
remains
unknown
because
their
recalcitrance
experimental
manipulation.
Here,
we
report
a
metagenomic
metatranscriptomic
analysis
Xanthoria
parietina
at
different
developmental
stages.
We
identified
168
genomes
symbionts
lichen-associated
microbes
across
sampled
thalli,
including
representatives
green
three
classes
14
bacterial
phyla.
By
analyzing
occurrence
individual
species
thalli
diverse
environments,
defined
both
substrate-specific
core
lichen.
Metatranscriptomic
principal
fungal
symbiont
stages
lichen,
compared
with
axenically
grown
fungus,
revealed
differential
gene
expression
profiles
indicative
lichen-specific
transporter
functions,
specific
cell
signaling,
transcriptional
regulation,
secondary
metabolic
capacity.
Putative
immunity-related
proteins
structurally
conserved
secreted
resembling
pathogen
effectors
were
also
identified,
consistent
role
for
immunity
modulation
morphogenesis.
Fungal Genetics and Biology,
Journal Year:
2024,
Volume and Issue:
172, P. 103893 - 103893
Published: April 23, 2024
Chitin
is
an
essential
structural
component
of
fungal
cell
walls
composed
transmembrane
proteins
called
chitin
synthases
(CHSs),
which
have
a
large
range
reported
effects
in
ascomycetes;
however,
are
poorly
understood
agaricomycetes.
In
this
study,
evolutionary
and
molecular
genetic
analyses
chs
genes
were
conducted
using
genomic
information
from
nine
ascomycete
six
basidiomycete
species.
The
results
support
the
existence
seven
previously
classified
clades
discovery
three
novel
basidiomycete-specific
(BI-BIII).
agaricomycete
fungus
Pleurotus
ostreatus
was
observed
to
putative
genes,
four
basidiomycete-specific.
Three
these
specific
disrupted
P.
20b
strain
(ku80
disruptant)
through
homologous
recombination
transformants
obtained
(Δchsb2,
Δchsb3,
Δchsb4).
Despite
numerous
transformations
Δchsb1
unobtainable,
suggesting
disruption
gene
causes
crucial
negative
effect
ostreatus.
Disruption
chsb2-4
caused
sparser
mycelia
with
rougher
surfaces
shorter
aerial
hyphae.
They
also
increased
sensitivity
wall
membrane
stress,
thinner
walls,
overexpression
other
glucan
synthases.
These
distinct
roles
formation
hyphae
important
for
understanding
evolution
filamentous
fungi.
Applied and Environmental Microbiology,
Journal Year:
2022,
Volume and Issue:
88(23)
Published: Nov. 14, 2022
Cre1
is
an
important
transcription
factor
that
regulates
carbon
catabolite
repression
(CCR)
and
widely
conserved
across
fungi.
The
cre1
gene
has
been
extensively
studied
in
several
Ascomycota
species,
whereas
its
role
expression
regulation
the
Basidiomycota
species
remains
poorly
understood.
Here,
we
identified
investigated
of
Coprinopsis
cinerea,
a
basidiomycete
model
mushroom
can
efficiently
degrade
lignocellulosic
plant
wastes.
We
used
rapid
efficient
deletion
approach
based
on
PCR-amplified
split-marker
DNA
cassettes
together
with
vitro
assembled
Cas9-guide
RNA
ribonucleoproteins
(Cas9
RNPs)
to
generate
C.
cinerea
strains.
Gene
profiling
two
independent
mutants
showed
significant
deregulation
carbohydrate
metabolism,
cell
wall
degrading
enzymes
(PCWDEs),
plasma
membrane
transporter-related
factor-encoding
genes,
among
others.
Our
results
support
notion
that,
like
reports
ascomycetes,
orchestrates
CCR
through
combined
diverse
including
PCWDEs,
factors
positively
regulate
transporters
which
could
import
simple
sugars
induce
PWCDEs.
Somewhat
paradoxically,
though
accordance
other
Agaricomycetes,
genes
related
lignin
degradation
were
mostly
downregulated
mutants,
indicating
they
fall
under
different
than
PCWDEs.
data
presented
here
will
expand
our
knowledge
provide
functional
hypotheses
biomass
degradation.
IMPORTANCE
Mushroom-forming
fungi
include
some
most
degraders.
They
dead
materials
by
battery
lignin-,
cellulose-,
hemicellulose-,
pectin-degrading
enzymes,
encoding
are
tight
transcriptional
control.
One
highest-level
regulations
these
metabolic
known
as
repression,
orchestrated
Cre1,
ensures
costly
lignocellulose-degrading
enzyme
expressed
only
when
sources
(e.g.,
glucose)
not
available.
ortholog
litter
decomposer
Agaricomycete,
knocked
it
out,
characterized
changes
mutants.
dozen
lignocellulolytic
well
putative
target
cre1.
These
extend
Basidiomycota.
Microbiological Research,
Journal Year:
2024,
Volume and Issue:
284, P. 127736 - 127736
Published: April 22, 2024
Blue
light
is
an
important
signal
for
fungal
development.
In
the
mushroom-forming
basidiomycete
Schizophyllum
commune,
blue
detected
by
White
Collar
complex,
which
consists
of
WC-1
and
WC-2.
Most
our
knowledge
on
this
complex
derived
from
ascomycete
Neurospora
crassa,
where
both
WC-2
contain
GATA
zinc-finger
transcription
factor
domains.
basidiomycetes,
truncated
does
not
a
domain,
but
are
still
We
show
that
dimerization
happens
independent
in
S.
induction
required
promoter
binding
complex.
Furthermore,
transcription,
alone
always
sufficient
to
initiate
transcription.
For
its
function,
associates
directly
with
promoters
structural
genes
involved
mushroom
development,
like
hydrophobins,
also
promotes
expression
other
factors
play
role
Applied Microbiology and Biotechnology,
Journal Year:
2024,
Volume and Issue:
108(1)
Published: June 18, 2024
Abstract
Submerged
cultivation
of
edible
filamentous
fungi
(
Agaricomycetes
)
in
bioreactors
enables
maximum
mass
transfer
nutrients
and
has
the
potential
to
increase
volumetric
productivity
fungal
biomass
compared
solid
state
cultivation.
These
aspects
are
paramount
if
one
wants
range
bioactives
(e.g.
glucans)
convenient
time
frames.
In
this
study,
Trametes
versicolor
(M9911)
outperformed
four
other
tested
strains
(during
batch
cultivations
an
airlift
bioreactor).
This
strain
was
therefore
further
semi-continuous
Continuous
(driven
by
dilution
rate,
D
preferred
bioprocess
strategies
for
production.
We
examined
T.
at
rates
between
0.02
0.1
h
−1
.
A
0.87
g/L/h
obtained
with
a
but
lower
total
production
(cell
dry
weight,
CDW
8.7
g/L)
than
(12.3
g/L
0.04
vs
13.4
g/L,
).
However,
growth
resulted
very
short
fermentation
(18
h)
which
terminated
due
washout
(the
specific
exceeded
rate
biomass).
At
,
12.3
achieved
without
compromising
residence
(184
fermentation.
While
0.07
comparable
productivities
(0.5
g/L/h),
duration
only
85
h.
The
highest
glucan
content
cells
(27.8
as
percentage
CDW)
while
lowest
observed
grown
Key
points
•
reported
g/L/h.
Semi-continuous
h−1
biomass.
28%
glucans
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(32), P. 18271 - 18282
Published: July 29, 2024
(enokitake)
is
widely
recognized
for
its
nutritional
and
medicinal
properties.
Understanding
the
biochemical
processes,
such
as
lipid
metabolism
during
fruiting
body
formation,
essential
enhancing
mushroom
cultivation
utilization.
This
study
aimed
at
elucidating
dynamic
lipidomic
changes
seven
growth
stages
of
