Biotechnological potential of salt tolerant and xerophilic species of Aspergillus DOI Creative Commons
István Pócsi, Jan Dijksterhuis, Jos Houbraken

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Nov. 19, 2024

Abstract Xerophilic fungi occupy versatile environments owing to their rich arsenal helping them successfully adapt water constraints as a result of low relative humidity, high-osmolarity, and high-salinity conditions. The general term xerophilic relates organisms that tolerate and/or require reduced activity, while halophilic osmophilic are applied specialized groups high salt concentrations or increased osmotic pressure, respectively. Species belonging the family Aspergillaceae , especially those classified in Aspergillus subgenus (sections Restricti ) Polypaecilum particularly enriched group salt-tolerant filamentous fungi. They produce an unprecedently wide spectrum tolerant enzymes including proteases, peptidases, glutaminases, γ-glutamyl transpeptidases, various glycosidases such cellulose-decomposing starch-degrading hydrolases, lipases, tannases, oxidareductases. These extremophilic also represent huge untapped treasure chest yet-to-be-discovered, highly valuable, biologically active secondary metabolites. Furthermore, these indispensable agents decolorizing textile dyes, degrading xenobiotics removing excess ions high-salt environments. could play role fermentation processes at activity leading preparation daqu, meju, tea. Considering current future agricultural applications, Aspergilli may contribute biosolubilization phosphate soil amelioration stress crops. Transgenes from halophile find promising applications engineering drought-tolerant spoil feed food raise mycotoxin above permissible doses and, therefore, development novel preservation technologies against spp. is urgently needed. On other hand, some have been shown be biological control mites. Key points • Salt can found resources valuable metabolites Biotechnological expanding

Language: Английский

Morpho-Molecular and Genomic Characterization of Penicillium mexicanum Isolates Retrieved from a Forsaken Gold Mine DOI Creative Commons
João Trovão, Fabiana Soares, Diana Sofia Paiva

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(22), P. 10600 - 10600

Published: Nov. 17, 2024

During the ongoing studies designed to examine fungal diversity present within abandoned and flooded Escádia Grande gold mine (Góis, Portugal), we repeatedly isolated several specimens belonging a Penicillium species. Molecular phylogenetic analysis, coupled with morphological observations, positioned this fungus subgen. sect. Paradoxa, series Atramentosa, pinpointing its identity as mexicanum (the first record for mining soils country). Given limited research conducted on Penicillia from similar environments, species genome was sequenced utilizing Oxford Nanopore® MinION™ methodology studied through bioinformatic analysis. The obtained has size of 29.62 Mb, containing 47.72% GC content, 10,156 genes, 44 rRNAs 178 tRNAs/tmRNAs, providing genomic resource microorganism. Bioinformatic analysis allowed us identify multiple traits that can contribute towards survival in these extreme including presence high levels major facilitator transporters (MFS), Zn (2)-C6 fungal-type DNA-binding domains, P-loop nucleoside triphosphate hydrolases, specific transcription factors sugar transporters. Furthermore, putative advantageous metabolic traits, such methylotrophy, assimilatory nitrate sulfate reduction abilities, were also detected. In addition, results highlighted strong organization investment arsenic detoxification (transport oxidation). Lastly, thirty-two biosynthetic gene clusters predicted, some similarity values monascorubrin, nidulanin A, histidyltryptophanyldiketopiperazine/dehydrohistidyltryptophanyldiketopiperazine/roquefortine D/roquefortine C/glandicoline A/glandicoline B/meleagrine, YWA1 choline. Overall, study expands current knowledge environments while enhancing our understanding regarding resistance.

Language: Английский

Citations

0
Genomic localization bias of secondary metabolite gene clusters and association with histone modifications inAspergillus DOI Creative Commons
Xin Zhang,

Iseult Leahy,

Jérôme Collemare

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 22, 2024

Abstract Fungi are well-known producers of bioactive secondary metabolites (SMs), which have been exploited for decades by humankind various medical applications like therapeutics and antibiotics. SMs synthesized biosynthetic gene clusters (BGCs) – physically co-localized co-regulated genes. Because BGCs often regulated histone post-translational modifications (PTMs), it was suggested that their chromosomal location is important expression. Studies in a few fungal species indicated an enrichment sub-telomeric regions; however, there no evidence with distinct genomic localization different PTMs. Here, we used 174 Aspergillus covering 22 sections to determine the correlation between BGC localization, expression We found high abundance diversity SM backbone genes across genus, notable unique within sections. Being or conserved many species, showed strong bias being localized low-synteny regions, regardless position chromosomes. Using chromosome-level assemblies, also confirmed significantly biased regions. Notably, regions about half those exhibit higher variability, likely due similar variability H3K4me3 H3K36me3 PTMs; while variations H3 acetylation H3K9me3 not correlated variation, as analyzed two species. Expression four further supports tend be located regulation involves PTMs than most commonly studied modifications. Significance known producing array compounds benefits, yet our understanding how production these remains limited. focused on genus , containing prolific compounds, systematically uncover pathways. By expanding knowledge beyond this research offers novel insights into pathways matters Thanks new view relation modifications, results expected stimulate functional neglected will support discovery harnessing industrial applications.

Language: Английский

Citations

0
Re-identification of Strains from Aspergillus Section Aspergillus and Description of Three Unrecorded Species from Korea DOI Creative Commons
Anbazhagan Mageswari, Daseul Lee, Le Dinh Thao

et al.

Mycobiology, Journal Year: 2024, Volume and Issue: 52(5), P. 267 - 277

Published: Sept. 2, 2024

The section

Language: Английский

Citations

0
Biotechnological potential of salt tolerant and xerophilic species of Aspergillus DOI Creative Commons
István Pócsi, Jan Dijksterhuis, Jos Houbraken

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Nov. 19, 2024

Abstract Xerophilic fungi occupy versatile environments owing to their rich arsenal helping them successfully adapt water constraints as a result of low relative humidity, high-osmolarity, and high-salinity conditions. The general term xerophilic relates organisms that tolerate and/or require reduced activity, while halophilic osmophilic are applied specialized groups high salt concentrations or increased osmotic pressure, respectively. Species belonging the family Aspergillaceae , especially those classified in Aspergillus subgenus (sections Restricti ) Polypaecilum particularly enriched group salt-tolerant filamentous fungi. They produce an unprecedently wide spectrum tolerant enzymes including proteases, peptidases, glutaminases, γ-glutamyl transpeptidases, various glycosidases such cellulose-decomposing starch-degrading hydrolases, lipases, tannases, oxidareductases. These extremophilic also represent huge untapped treasure chest yet-to-be-discovered, highly valuable, biologically active secondary metabolites. Furthermore, these indispensable agents decolorizing textile dyes, degrading xenobiotics removing excess ions high-salt environments. could play role fermentation processes at activity leading preparation daqu, meju, tea. Considering current future agricultural applications, Aspergilli may contribute biosolubilization phosphate soil amelioration stress crops. Transgenes from halophile find promising applications engineering drought-tolerant spoil feed food raise mycotoxin above permissible doses and, therefore, development novel preservation technologies against spp. is urgently needed. On other hand, some have been shown be biological control mites. Key points • Salt can found resources valuable metabolites Biotechnological expanding

Language: Английский

Citations

0