Morpho-Molecular and Genomic Characterization of Penicillium mexicanum Isolates Retrieved from a Forsaken Gold Mine DOI Creative Commons
João Trovão, Fabiana Soares, Diana Sofia Paiva

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(22), P. 10600 - 10600

Published: Nov. 17, 2024

During the ongoing studies designed to examine fungal diversity present within abandoned and flooded Escádia Grande gold mine (Góis, Portugal), we repeatedly isolated several specimens belonging a Penicillium species. Molecular phylogenetic analysis, coupled with morphological observations, positioned this fungus subgen. sect. Paradoxa, series Atramentosa, pinpointing its identity as mexicanum (the first record for mining soils country). Given limited research conducted on Penicillia from similar environments, species genome was sequenced utilizing Oxford Nanopore® MinION™ methodology studied through bioinformatic analysis. The obtained has size of 29.62 Mb, containing 47.72% GC content, 10,156 genes, 44 rRNAs 178 tRNAs/tmRNAs, providing genomic resource microorganism. Bioinformatic analysis allowed us identify multiple traits that can contribute towards survival in these extreme including presence high levels major facilitator transporters (MFS), Zn (2)-C6 fungal-type DNA-binding domains, P-loop nucleoside triphosphate hydrolases, specific transcription factors sugar transporters. Furthermore, putative advantageous metabolic traits, such methylotrophy, assimilatory nitrate sulfate reduction abilities, were also detected. In addition, results highlighted strong organization investment arsenic detoxification (transport oxidation). Lastly, thirty-two biosynthetic gene clusters predicted, some similarity values monascorubrin, nidulanin A, histidyltryptophanyldiketopiperazine/dehydrohistidyltryptophanyldiketopiperazine/roquefortine D/roquefortine C/glandicoline A/glandicoline B/meleagrine, YWA1 choline. Overall, study expands current knowledge environments while enhancing our understanding regarding resistance.

Language: Английский

Insights from Cutting-edge Diagnostics and Epidemiology of Sporotrichosis and Taxonomic Shifts in Sporothrix DOI

Tiago Ribeiro Machado,

Sarah Santos Gonçalves, Jamile Ambrósio de Carvalho

et al.

Current Fungal Infection Reports, Journal Year: 2025, Volume and Issue: 19(1)

Published: Feb. 18, 2025

Language: Английский

Citations

2
Three New Records of Pathogens Causing Stem Blight on Vaccinium corymbosum in China DOI Creative Commons
Yueyan Zhou,

Linna Wu,

K. J. Ren

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 647 - 647

Published: Feb. 20, 2025

Stem blight is a significant disease affecting blueberries worldwide, caused by various pathogens. This study investigated stem in Ji’an, Jilin Province, China. Fungi isolated from diseased stems were identified as Colletotrichum temperatum, Curvularia austriaca, and Diaporthe unshiuensis based on morphological characters multi-locus phylogenetic analyses using the internal transcribed spacer (ITS) region, glyceraldehyde 3-phosphate dehydrogenase (gapdh), chitin synthase (chs), actin (act), β-tubulin (tub2), translation elongation factor 1-alpha (tef1-α), calmodulin (cal), histone 3 (his3) regions. Pathogenicity test was conducted detached green blueberry shoots, all shoots inoculated mycelium plugs presented necrotic lesions with dark brown margins, while control (PDA plugs) group did not show any symptoms. Koch’s postulates confirmed re-isolating pathogen The provides three new host–pathogen records of fungi associated blight.

Language: Английский

Citations

1
Genetic diversity and molecular evolution of 3-carboxymuconate cyclase (Gp60–70), the major antigen in pathogenic Sporothrix species DOI Creative Commons
Jamile Ambrósio de Carvalho, Thiago Costa Machado,

Alexandre Augusto Sasaki

et al.

Mycology&#58 An International Journal on Fungal Biology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 27

Published: March 8, 2025

Language: Английский

Citations

1
NCBI RefSeq: reference sequence standards through 25 years of curation and annotation DOI Creative Commons

Tamara Goldfarb,

Vamsi K. Kodali, Shashikant Pujar

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 53(D1), P. D243 - D257

Published: Nov. 11, 2024

Reference sequences and annotations serve as the foundation for many lines of research today, from organism sequence identification to providing a core description genes, transcripts proteins found in an organism's genome. Interpretation data including transcriptomics, proteomics, variation comparative analyses based on reference gene informs our understanding function possible disease mechanisms, leading new biomedical discoveries. The Sequence (RefSeq) resource created at National Center Biotechnology Information (NCBI) leverages both automatic processes expert curation create robust set genomic, transcript protein spanning tree life. RefSeq continues refine its annotation quality control utilize better genomes resulting advances sequencing technologies well RNA-Seq produce high-quality annotated genomes, ortholog predictions across more organisms other products that are easily accessible through multiple NCBI resources. This report summarizes current status eukaryotic, prokaryotic viral resources, with focus eukaryotic annotation, increase taxonomic representation effect it will have genomics. is publicly https://www.ncbi.nlm.nih.gov/refseq.

Language: Английский

Citations

8
Exploring the Diversity and Ecological Dynamics of Palm Leaf Spotting Fungi—A Case Study on Ornamental Palms in Portugal DOI Creative Commons
Diana S. Pereira, Alan J. L. Phillips

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 43 - 43

Published: Jan. 7, 2025

Palm trees (Arecaceae) are among the most popular ornamental plants worldwide. Despite extensive research on fungi associated with Arecaceae, diversity and ecological dynamics of affecting palms remain poorly studied, although they have significant impact palm health economic value. Furthermore, while fungal has traditionally focused tropical assemblages, in temperate climates offer a unique opportunity to explore non-native habitats. The present study conducted preliminary assessment ecology potential phytopathogenic foliar lesions various host species Portugal, combining morphological examination, PCR-based genomic fingerprinting, biodiversity data analysis. examination 134 sampled from 100 resulted collection 2064 leaf spotting (PLSF), representing diverse assemblage 320 molecular operational taxonomic units (MOTUs) across 97 genera. overall community composition revealed distinct dominated by Neosetophoma, Alternaria, Phoma, Cladosporium, profusion infrequent rare taxa consistent logseries distribution. Significantly positive co-occurrence (CO) patterns prevalent uncommon suggest synergistic interactions enhancing colonisation, persistence, pathogenicity. structures PLSF contrasted markedly fungi, especially prevalence pleosporalean coelomycetes Didymellaceae Phaeosphaeriaceae, including recently introduced or not previously documented genera Arecaceae. This novel suggests that climatic constraints shape structure communities, resulting distinctive assemblages. In addition, assemblages varied significantly species, temperate-native hosting more diverse, coelomycete-enriched communities. findings highlight as hyperdiverse microhabitats harbouring communities intricate complex interplay climatic, host, factors. With climate change altering environmental conditions, identification thriving inhabiting these becomes crucial for predicting shifts pathogen mitigating future disease outbreaks. Understanding is essential identifying threats developing effective management strategies sustainability plants.

Language: Английский

Citations

0
Morphological and phylogenetic analyses reveal two new species of the Fusarium fujikuroi (Hypocreales, Nectriaceae) species complex in China DOI Creative Commons

Mingwei Zhang,

Cheng Peng, Shuji Li

et al.

MycoKeys, Journal Year: 2025, Volume and Issue: 112, P. 127 - 163

Published: Jan. 16, 2025

The Fusarium fujikuroi species complex (FFSC) encompasses a diverse array of more than 80 phylogenetic with both phytopathological and clinical importance. A stable taxonomy is crucial for in the FFSC due to their economical relevance. Fungal strains used this study were obtained from Castanea mollissima Rubus lambertianus , collected Beijing Shaanxi Province. We employ morphological analyses based on partial gene fragments translation elongation factor 1-alpha ( tef1 ), beta-tubulin tub2 calmodulin CaM RNA polymerase largest subunit rpb1 II second rpb2 as well pairwise homoplasy index tests. Studies have shown that these phylospecies are clustered Asian clade FFSC. present delineates two novel within FFSC, named F. castaneophilum rubicola complemented by illustrations descriptions.

Language: Английский

Citations

0
Carbon-Free Fungicide for Sustainable Agriculture: An Efficient Niobium Compound against Foliar Fungi DOI
Cinthia C. Oliveira, Ana Pacheli Heitmann,

M. Fontana

et al.

ACS Agricultural Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Among the daunting challenges to sustainability of agricultural sector is development environmentally sound technologies driving food security and higher yields together with an integrated "One Health" approach. This work explores a new niobium molecule, PNB53, as potent antifungal agent potential benefits for productivity low environmental impact. In vitro tests were performed evaluate efficiency PNB53 in controlling target nontarget fungi by production reactive oxygen species. Greenhouse field experiments on soybean crops conducted check activity effects productivity, comparison commercial fungicides. The results showed that presented significant decrease damage caused Asian rust (Phakopsora pachyrhizi) crop, 61% efficacy, better against Corynespora cassiicola (42.4%) than fungicides (39.4%). biological control fungus Trichoderma harzianum was not affected doses dose vitro. productivities 4531, 4465, 3446 kg ha–1, respectively, fungicide, untreated check. Thus, 140 g ha–1 promoted yield increase 18.1 bags per hectare over plots, indicating positive physiological effect crops. view growing demand supplies commensurate population growth, niobium-based compounds can offer great contribution become alternative sustainable agriculture.

Language: Английский

Citations

0
Molecular Tracking of Emerging Fusarium Species in Keratitis: F. veterinarium, F. contaminatum, and F. curvatum DOI
Ruan Campos Monteiro, Maria Cecília Zorat Yu, Somayeh Dolatabadi

et al.

Mycopathologia, Journal Year: 2025, Volume and Issue: 190(1)

Published: Feb. 1, 2025

Language: Английский

Citations

0
Would global warming bring an increase of invertebrate-associated cutaneous invasive fungal infections? DOI Creative Commons
Dimitrios P. Kontoyiannis, Arturo Casadevall

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

ABSTRACT Invasive mold-associated cutaneous disease is a rare but potentially catastrophic consequence of trauma. However, invertebrate bites are not well recognized as mechanism for the inoculation fungi into subcutaneous tissue that can also result in severe infections. Invertebrates often carry with human pathogenic potential part their microbiome, and break skin, providing conduit them to penetrate tissues where establishment infection produce serious skin soft fungal diseases. In this essay, we review existing data bite-associated invasive infections (IBA-cIFIs) consider consequences global warming on epidemiology. Climate changes will be associated range invertebrates adaptation microbes warmer temperatures. Fungal higher temperatures defeat mammalian protective barrier both more different IBA-cIFIs.

Language: Английский

Citations

0
Molecular phylogeny and taxonomy reveal two new genera and five new species in Phanerochaetaceae (Polyporales) from Yunnan, Southwest China DOI Creative Commons
Ying Xu, Yang Yang, XIN YANG

et al.

MycoKeys, Journal Year: 2025, Volume and Issue: 113, P. 263 - 294

Published: Feb. 12, 2025

In the present study, two new genera Paradonkia, and Neodonkiella, five species, viz. Paradonkiafarinacea, Neodonkiellayinjiangensis, Phanerochaetealbocremea, Phanerochaetefissurata, Phanerochaetepunctata collected from southern China, are proposed based on a combination of morphological features molecular evidence. Paradonkiafarinacea is characterized by resupinate, membranaceous basidiomata with pale cream to gray hymenial surface monomitic hyphal system simple septa clamp connections; Neodonkiellayinjiangensis soft coriaceous basidiomata, ellipsoid basidiospores (3.5-5 × 2-2.5 µm); Phanerochaetealbocremea resupinate white surface, 2-3 Phanerochaetefissurata gray-brown cracked (4-5.5 µm) farinaceous system, basidiospores. Sequences internal transcribed spacers (ITS) large subunit (nLSU) nuclear ribosomal DNA (rDNA) markers studied samples were generated. Phylogenetic analyses performed using maximum likelihood, parsimony, Bayesian inference methods. The phylogram ITS+nLSUrDNA gene regions, revealed that genera, Paradonkia belong family Phanerochaetaceae, three species genus Phanerochaete in Phanerochaetaceae.

Language: Английский

Citations

0