Comparison of radial immunodiffusion, turbidimetric immunoassay, and Brix refractometry for determining bovine colostrum quality

JDS Communications, Journal Year: 2024, Volume and Issue: 5(6), P. 679 - 683

Published: July 14, 2024

Determining the concentration of IgG in colostrum is critical for assessment quality. On-farm use a Brix refractometer to estimate widespread whereas radial immunodiffusion (RID) laboratory reference method. Turbidimetric immunoassay (TIA) might offer an alternative method quantify colostrum, but agreement with RID, as well thresholds determine high-quality remain uncertain. The objective this study was level between %, and TIA evaluation Composite samples (n = 58) from Holstein cows were evaluated using digital at time collection stored −20°C until analysis. determined RID TIA. Data analyzed Passing-Bablok regression Bland-Altman plots. Critical measurements identify ≥ 50 100 g/L based on logistic receiver operating characteristic curves. Results revealed that both (Kendall's tau (Τ) 0.91) % (Τ 0.78) had strong correlation respectively. identified systematic [6.91 (4.33 8.98) g/L] proportional [0.69 (0.67 0.72) bias optimum threshold 40.6 (area under curve (AUC): 1.0; Sensitivity (Se): 100; Specificity (Sp): 100) 85.8 (AUC: 0.99; Se: 96.6; Sp: 96.6) 18.4% 25.8% 82.8; 93.1) Using thresholds, our results show highly accurate identifying because bias, direct comparison obtained by are cautioned.

Language: Английский

Invited Review: Factors influencing colostrum production of multiparous Holstein and Jersey cows*

Applied Animal Science, Journal Year: 2024, Volume and Issue: 40(6), P. 791 - 801

Published: Dec. 1, 2024

Language: Английский