Expression of 9-O-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells DOI Creative Commons

Hugo Sánchez-Martínez,

Victoria Jiménez-Castillo,

Daniela Illescas-Barbosa

et al.

Life, Journal Year: 2025, Volume and Issue: 15(4), P. 663 - 663

Published: April 17, 2025

Oral squamous cell carcinoma (OSCC) is a common type of head and neck malignancy that represents significant global health issue. Sialylations are events in tumor transformation, proliferation, metastasis, immune evasion. Modifications sialylation can be detected by lectins, whose changes OSCC have been related to grade, invasion, metastasis. The presence 9-O-acetylated sialic acid (Neu5,9Ac2) cells its potential expression, modification, role unknown. This study aimed analyze the expression Neu5,9Ac2 using Macrobrachium rosenbergii lectin (MrL) recognizes this (Neu5Ac) residue also compare effect on SCC-152 line (CRL-3240, ATCC) immortalized keratinocytes (HaCaT) as control. We observed immunocytochemistry expressed more compared HaCaT cells; specificity MrL was confirmed after sialidase treatment which loss lectin’s recognition observed. electrophoretic profile similar between both types; however, Western blot showed differences glycoprotein patterns recognized for each type. increased proliferation cells, well integrity morphology colonies. Therefore, our results suggest glycosylated receptors could involved survival offers promising avenue developing diagnostic prognostic tools (tumor markers) against oral future.

Language: Английский

Snake venom galactoside-binding lectin from Bothrops jararacussu: Special role in leukocytes activation and function DOI
Juliana P. Zuliani, Fábio Henrique Kwasniewski, Yoda Janaina Ikenohuchi

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 296, P. 139742 - 139742

Published: Jan. 10, 2025

Language: Английский

Citations

0
Glycosylation profiling of triple-negative breast cancer: clinical and immune correlations and identification of LMAN1L as a biomarker and therapeutic target DOI Creative Commons
Qianru Yu,

Hanyi Zhong,

Xinhao Zhu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Breast cancer (BC) is the most prevalent malignant tumor in women, with triple-negative breast (TNBC) showing poorest prognosis among all subtypes. Glycosylation increasingly recognized as a critical biomarker microenvironment, particularly BC. However, glycosylation-related genes associated TNBC have not yet been defined. Additionally, their characteristics and relationship deeply investigated. Transcriptomic analyses were used to identify signature (GRS) prognosis. A machine learning-based prediction model was constructed validated across multiple independent datasets. The model's predictive capability extended evaluate of individuals, immune microenvironment immunotherapy response. LMAN1L (Lectin, Mannose Binding 1 Like) identified novel prognostic marker TNBC, its biological effects through experimental assays. GRS showed significant relevance for patients. risk effectively predicted molecular features, including cell infiltration potential responses immunotherapy. Experimental validation confirmed gene, low expression significantly inhibiting proliferation migration. Our demonstrates robust This offers promising strategy personalized treatment improved clinical outcomes TNBC.

Language: Английский

Citations

0
The application of snake venom in anticancer drug discovery: an overview of the latest developments DOI

J. R. da Silva,

Juliana Castro-Amorim, Ashis K. Mukherjee

et al.

Expert Opinion on Drug Discovery, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 19

Published: Feb. 26, 2025

Snake venom is a rich source of toxins with great potential for therapeutic applications. In addition to its efficacy in treating hypertension, acute coronary syndrome, and other heart conditions, research has shown that this potent enzymatic cocktail capable selectively targeting destroying cancer cells many cases while sparing healthy cells. The authors begin by acknowledging the emerging trends snake-derived targeted therapies battling cancer. An extensive literature review examining effects various snake on cell lines, highlighting specific hallmarks each toxin targets presented. Furthermore, emphasize artificial intelligence accelerating venom-based drug discovery treatment, showcasing several innovative software applications field. Research indicates promising treatment as discussed can specifically target Nevertheless, variations composition venoms, ethical issues, delivery barriers limit their development into effective therapies. Thus, advances biotechnology, molecular engineering, silico methods are crucial refinement venom-derived compounds, improving specificity, overcoming these challenges, ultimately enhancing therapy.

Language: Английский

Citations

0
Maackia amurensis seed lectin (MASL) structure and sequence comparison with other Maackia amurensis lectins DOI Creative Commons
Ashok R. Nayak,

Cayla J. Holdcraft,

Ariel C. Yin

et al.

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108466 - 108466

Published: March 1, 2025

Maackia amurensis lectins, including MASL, MAA, and MAL2, are widely utilized in biochemical medicinal research. However, the structural functional differences between these lectins have not been defined. Here, we present a high-resolution cryo-EM structure of MASL revealing that its tetrameric assembly is directed by two intersubunit disulfide bridges. These bridges, formed C272 residues, central to dimer-of-dimers tetramer. This also identifies residues involved stabilizing dimer interface, multiple glycosylation sites, calcium manganese atoms sugar-binding pockets MASL. Notably, our analysis reveals Y250 carbohydrate-binding site adopts flipped conformation, likely acting as gatekeeper obstructs access non-cognate substrates, feature may contribute MASL's substrate specificity. Sequence suggests MAA truncated version while MAL2 represents homologous isoform. Unlike neither nor contains cysteine residue required for bridge formation. Accordingly, proteins using reducing nonreducing SDS-PAGE confirms drives intermolecular findings provide critical insights into unique features distinguish it from other offering foundation further exploration biological therapeutic potential.

Language: Английский

Citations

0
Expression of 9-O-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells DOI Creative Commons

Hugo Sánchez-Martínez,

Victoria Jiménez-Castillo,

Daniela Illescas-Barbosa

et al.

Life, Journal Year: 2025, Volume and Issue: 15(4), P. 663 - 663

Published: April 17, 2025

Oral squamous cell carcinoma (OSCC) is a common type of head and neck malignancy that represents significant global health issue. Sialylations are events in tumor transformation, proliferation, metastasis, immune evasion. Modifications sialylation can be detected by lectins, whose changes OSCC have been related to grade, invasion, metastasis. The presence 9-O-acetylated sialic acid (Neu5,9Ac2) cells its potential expression, modification, role unknown. This study aimed analyze the expression Neu5,9Ac2 using Macrobrachium rosenbergii lectin (MrL) recognizes this (Neu5Ac) residue also compare effect on SCC-152 line (CRL-3240, ATCC) immortalized keratinocytes (HaCaT) as control. We observed immunocytochemistry expressed more compared HaCaT cells; specificity MrL was confirmed after sialidase treatment which loss lectin’s recognition observed. electrophoretic profile similar between both types; however, Western blot showed differences glycoprotein patterns recognized for each type. increased proliferation cells, well integrity morphology colonies. Therefore, our results suggest glycosylated receptors could involved survival offers promising avenue developing diagnostic prognostic tools (tumor markers) against oral future.

Language: Английский

Citations

0