International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113806 - 113806
Published: Dec. 15, 2024
Language: Английский
PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18945 - e18945
Published: Feb. 13, 2025
mRNA expression-based stemness index (mRNAsi) has been used for prognostic assessment in various cancers, but its application lung adenocarcinoma (LUAD) is limited, which the focus of this study. Low mRNAsi LUAD predicted a better prognosis. Eight genes ( GNG7, EIF5A, ANLN, FKBP4, GAPDH, GNPNAT1, E2F7, CISH ) associated with were screened to establish risk model. The differentially expressed between high and low groups mainly enriched metabolism, cell cycle functions pathway. score group had higher immune scores. Patients lower TIDE scores immunotherapy outcomes. In addition, was effective assessing drug sensitivity LUAD. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) data showed that eight lines, knockdown EIF5A reduced invasion migration ability cells. This study designed model based on mRNAsi-related predicting accurately prognosis survival patients, facilitating patients chemotherapy.
Language: Английский
Citations
0
Heliyon, Journal Year: 2025, Volume and Issue: unknown, P. e42961 - e42961
Published: Feb. 1, 2025
Language: Английский
Citations
0
Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 8, 2025
Hepatocellular carcinoma (HCC) is one of the leading causes cancer-related death worldwide. Although many therapeutic modalities have been established for treating HCC patients, outcomes patients remain unsatisfactory. Development independent prognostic biomarkers thus an important need to allow early diagnosis and timely treatment. MicroRNAs (miRNAs) are most studied class small non-coding RNAs. It has shown that miRNAs play essential roles in multiple steps tumorigenesis progression. Furthermore, baseline expression levels altered tumor tissues blood circulation patients. Therefore, emerged as prediction prognosis. This review provides a comprehensive literature-based summary tissue circulating miRNA with significance HCC.
Language: Английский
Citations
0
International Journal of Genomics, Journal Year: 2025, Volume and Issue: 2025(1)
Published: Jan. 1, 2025
Background: The prognosis for lung adenocarcinoma (LUAD) is poor, and the recurrence rate high. Thus, to evaluate patients’ prognoses direct therapy choices, new prognostic markers are desperately needed. Methods: First, gene modules associated with LUAD were identified by weighted coexpression network analysis (WGCNA) analysis. expression profiles obtained intersected differential expressed genes taken between samples paracancerous samples. Afterward, stepwise regression LASSO used compress further, a risk model was created. Furthermore, nomogram based on scores clinical features created validate model. After that, distinctions pertinent biological processes signaling pathways among various subgroups investigated. Additionally, drug sensitivity testing, immunotherapy, immune infiltration analysis, enrichment carried out. Finally, role of ANLN in explored qPCR, cell scratch assay, transwell. Results: A total 257 taking intersection 2866 paraneoplastic module after we screened two particular that had strongest link WGCNA. , CASS4 NMUR1 found be distinctive development models intersecting find 176 linked LUAD. Based assessments, high‐ low‐risk groups patients divided. Low‐risk exhibited significantly higher overall survival (OS) than those high‐risk group. Expression correlates vast majority cells. Significant differences pathways, microenvironment, abundance groups. study showed group IC 50 values BMS‐754807_2171 Doramapimod_10424. vitro experiments demonstrated knocking down noticeably inhibited viability, migration, invasion A549 Conclusion: This may provide theoretical reference future exploration potential diagnostic biomarkers
Language: Английский
Citations
0
CytoJournal, Journal Year: 2024, Volume and Issue: 21, P. 77 - 77
Published: Dec. 27, 2024
Lung cancer, as a prevalent malignancy, continues to be considerable clinical challenge. This study aimed elucidate the role of microRNA-5703 (miR-5703) in lung cancer progression and assess effect exosomal miR-5703 on tumor-associated endothelial cells (TAECs). We analyzed Gene Expression Omnibus datasets performed quantitative real-time polymerase chain reaction determine expression levels tissues. Exosomes derived from were identified, effects inhibitors or mimics malignant biological behavior evaluated cells. Moreover, understand these TAECs, we assessed angiogenesis, endothelial-mesenchymal transition (EndMT), barrier function after treatment with miR- 5703 exosome-assimilated inhibitor cytochalasin D. Tumor-bearing mouse models used validating tumor-promoting exosomes cells, markers EndMT, examined. Our results showed that was up-regulated patient-derived exosomes. facilitated cell growth, migration, invasion, LC impaired function, which promoted angiogenesis EndMT TAECs by carrying through targeting growth family member 4 (ING4) identified target (P < 0.05). In vivo, cell-derived rescued inhibitors, leading up-regulation ING4 reduction vascular distribution tumor tissues operates an oncogenic factor cancer. After being taken up promotes damage ING4. Hence, is potential microenvironment.
Language: Английский
Citations
1
International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113806 - 113806
Published: Dec. 15, 2024
Language: Английский
Citations
0