Insights into the Gene Expression Profile of Classical Hodgkin Lymphoma: A Study towards Discovery of Novel Therapeutic Targets DOI Creative Commons
Abdulaziz A. Aloliqi

Molecules, Journal Year: 2024, Volume and Issue: 29(15), P. 3476 - 3476

Published: July 25, 2024

Classical Hodgkin lymphoma (cHL) is a common B-cell cancer and significant health concern, especially in Western Asian countries. Despite the effectiveness of chemotherapy, many relapse cases are being reported, highlighting need for improved treatments. This study aimed to address this issue by discovering biomarkers through analysis gene expression data specific cHL. Additionally, potential anticancer inhibitors were explored target discovered biomarkers. proceeded retrieving microarray from cHL patients, which was then analyzed identify differentially expressed genes (DEGs). Functional network annotation upregulated revealed active involvement matrix metallopeptidase 12 (MMP12) C-C motif ligand 22 (CCL22) progression mentioned found be actively involved cancer-related pathways, i.e., oxidative phosphorylation, complement pathway, myc_targets_v1 TNFA signaling via NFKB, etc., showed strong associations with other known promote progression. MMP12, topping list logFC value +6.6378, selected inhibition using docking simulation strategies. The compounds docked into site MMP12 molecular structure, revealing binding scores -7.7 kcal/mol -7.6 BDC_24037121 BDC_27854277, respectively. Simulation studies complexes further supported effective ligands, yielding MMGBSA MMPBSA -78.08 -82.05 MMP12-BDC_24037121 -48.79 -49.67 MMP12-BDC_27854277, Our findings highlight role cHL, effectively inhibiting its function potentially halting advancement Further exploration downregulated warranted, as associated may play CCL22 should considered investigation due

Language: Английский

TRIM36 serves as a prognostic indicator linked to immune infiltration in KIRC DOI Creative Commons

Jikai Zhang,

B.S. Zou,

Yun-Feng Geng

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42540 - e42540

Published: Feb. 1, 2025

Renal cell carcinoma (RCC) represents approximately 85% of all renal malignant tumors, with kidney clear (KIRC) being the most typical subtype. The tripartite motif (TRIM) family is involved in cancer initiation, progression, and therapy resistance. While TRIM36 has exhibited anti-tumor effects various cancers, its relationship KIRC remains unclear. In our research, we studied between KIRC. Through a combination bioinformatic analyses validation experiments, noted rise expression KIRC, upregulation associated poorer prognosis Also, findings from wound healing assays transwell migration showed that promotes proliferation cells. To understand underlying mechanisms, screened relevant genes conducted enrichment analysis. We identified may interact 5 hub involve cycle division processes Additionally, through immune infiltration analysis, found 6 tumor-infiltrating lymphocytes (TILs) inhibitors. summary, research identifies as promising biomarker comprehensively explores promoting effect on

Language: Английский

Citations

0
Proteomics and lipidomics of human umbilical cord mesenchymal stem cells exposed to ionizing radiation DOI Creative Commons
Dongmei Han, Ding Li, Xiao-Li Zheng

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 28, 2025

Mesenchymal stem cell (MSC)-based therapies exhibit beneficial effects on various forms of tissue damage, including ionizing radiation-induced lesions. However, whether radiation affects the functions human umbilical cord mesenchymal cells (hucMSCs) remains unclear. This study aimed to investigate effect and possible mechanisms proliferation differentiation hucMSCs. The hucMSCs were divided into 1 Gy group (exposure a single dose (1 Gy) X-ray Gy/min) for 14 days) control (without treatment) group. proliferation, apoptosis, adipogenic osteogenic abilities in two groups evaluated. Moreover, lipidomics proteomics analyses conducted explore crucial lipids proteins by which affected In addition, BYSL radiation-treated explore, as well involved potential mechanisms. treatment inhibited promoted decreased Key lipids, such triglyceride (TG) phosphatidylcholine (PC), hub (BYSL, MRTO4, RRP9) exhibited significant differences between BYSL, RRP9 significantly correlated with TG PC. overexpression evidently hucMSCs, protein expression levels p-GSK-3β/GSK-3β β-catenin, while suppressed apoptosis. GSK-3β inhibitor (1-Az) reversed p-GSK-3β/GSK-3β, β-catenin Our findings reveal that are radiation, may be associated changes key (TG PC) RRP9). Furthermore, promotes via GSK-3β/β-catenin pathway. These help explain response have clinical implications improving outcomes MSC-based after radiotherapy.

Language: Английский

Citations

0
Insights into the Gene Expression Profile of Classical Hodgkin Lymphoma: A Study towards Discovery of Novel Therapeutic Targets DOI Creative Commons
Abdulaziz A. Aloliqi

Molecules, Journal Year: 2024, Volume and Issue: 29(15), P. 3476 - 3476

Published: July 25, 2024

Classical Hodgkin lymphoma (cHL) is a common B-cell cancer and significant health concern, especially in Western Asian countries. Despite the effectiveness of chemotherapy, many relapse cases are being reported, highlighting need for improved treatments. This study aimed to address this issue by discovering biomarkers through analysis gene expression data specific cHL. Additionally, potential anticancer inhibitors were explored target discovered biomarkers. proceeded retrieving microarray from cHL patients, which was then analyzed identify differentially expressed genes (DEGs). Functional network annotation upregulated revealed active involvement matrix metallopeptidase 12 (MMP12) C-C motif ligand 22 (CCL22) progression mentioned found be actively involved cancer-related pathways, i.e., oxidative phosphorylation, complement pathway, myc_targets_v1 TNFA signaling via NFKB, etc., showed strong associations with other known promote progression. MMP12, topping list logFC value +6.6378, selected inhibition using docking simulation strategies. The compounds docked into site MMP12 molecular structure, revealing binding scores -7.7 kcal/mol -7.6 BDC_24037121 BDC_27854277, respectively. Simulation studies complexes further supported effective ligands, yielding MMGBSA MMPBSA -78.08 -82.05 MMP12-BDC_24037121 -48.79 -49.67 MMP12-BDC_27854277, Our findings highlight role cHL, effectively inhibiting its function potentially halting advancement Further exploration downregulated warranted, as associated may play CCL22 should considered investigation due

Language: Английский

Citations

0