History of carbon supply shapes the metabolic response of photogranules to light shifts DOI Creative Commons

Oriane Della-Negra,

Anaïs Séguéla,

Camille Guilmineau

et al.

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123557 - 123557

Published: March 1, 2025

Oxygenic photogranules mainly composed of cyanobacteria and heterotrophic bacteria, have gained attention for their ability to treat wastewater (removal C, N, P) without external aeration. Currently, the metabolic dynamics varying nutrient light conditions in treatment systems remains poorly studied. However, understanding how quickly photogranule metabolism changes, whether this change is temporary or permanent important optimal use photogranules. Here, an NMR-based metabolomics approach was applied investigate temporal presence absence acetate with light. Our findings revealed that under carbon-limited conditions, relied on alternative carbon sources, such as N-acetylneuraminate (a constituent EPS) amino acids like hypotaurine L-alanine. This adaptation affected key pathways, including glycolysis, taurine metabolism, tricarboxylic acid cycle. When provided, both phototrophic activities were maintained. Notably, history supply influenced responded shifts. Metabolic indicators showed lag addition altered fatty fixation, leading shifts concentrations distinct profiles when turned off. Thus, NMR identified induced by contrasting lasting several hours, significantly affecting response fluctuations. These results suggest supplementation may shape responses other environmental changes stressors.

Language: Английский

History of carbon supply shapes the metabolic response of photogranules to light shifts DOI Creative Commons

Oriane Della-Negra,

Anaïs Séguéla,

Camille Guilmineau

et al.

Water Research, Journal Year: 2025, Volume and Issue: unknown, P. 123557 - 123557

Published: March 1, 2025

Oxygenic photogranules mainly composed of cyanobacteria and heterotrophic bacteria, have gained attention for their ability to treat wastewater (removal C, N, P) without external aeration. Currently, the metabolic dynamics varying nutrient light conditions in treatment systems remains poorly studied. However, understanding how quickly photogranule metabolism changes, whether this change is temporary or permanent important optimal use photogranules. Here, an NMR-based metabolomics approach was applied investigate temporal presence absence acetate with light. Our findings revealed that under carbon-limited conditions, relied on alternative carbon sources, such as N-acetylneuraminate (a constituent EPS) amino acids like hypotaurine L-alanine. This adaptation affected key pathways, including glycolysis, taurine metabolism, tricarboxylic acid cycle. When provided, both phototrophic activities were maintained. Notably, history supply influenced responded shifts. Metabolic indicators showed lag addition altered fatty fixation, leading shifts concentrations distinct profiles when turned off. Thus, NMR identified induced by contrasting lasting several hours, significantly affecting response fluctuations. These results suggest supplementation may shape responses other environmental changes stressors.

Language: Английский

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