L-Arginine and Nitric Oxide in Vascular Regulation—Experimental Findings in the Context of Blood Donation

Nutrients, Journal Year: 2025, Volume and Issue: 17(4), P. 665 - 665

Published: Feb. 13, 2025

This narrative review provides an analysis of the role nitric oxide (NO) and its precursors, particularly L-arginine, in vascular regulation health, with emphasis on findings from our experimental research animal models. NO serves as a critical mediator function, contributing to vasodilation, blood flow, prevention thrombosis. As primary precursor NO, L-arginine is essential for maintaining endothelial integrity, modulating mitochondrial reducing oxidative damage. synthesises data contextualises these within physiological challenges faced by donors, such repeated donation associated stress. It examines effects supplementation respiration, lipid peroxidation, microsomal oxidation different conditions, including differences age, gender, dietary interventions. The mechanisms which enhances production, improves elasticity, alleviates dysfunction caused reduced bioavailability are also investigated. By integrating insights existing literature, this perspective potential address specific needs donors. highlights importance personalised nutritional approaches enhancing donor recovery resilience. In addition, assesses wider implications mitigating stress preserving function. interplay between bioavailability, factors, adaptation donors highlighted, along identification current knowledge gaps recommendations future research. presenting both original evidence synthesis article therapeutic promoting health context donation.

Language: Английский

Recent Issues in the Development and Application of Targeted Therapies with Respect to Individual Animal Variability

Animals, Journal Year: 2025, Volume and Issue: 15(3), P. 444 - 444

Published: Feb. 6, 2025

This literature review explores the impact of molecular, genetic, and environmental factors on efficacy targeted therapies in veterinary medicine. Relevant studies were identified through systematic searches PubMed, Web Science, Scopus, ScienceDirect using keywords such as “species-specific treatment strategies”, “signalling pathways”, “epigenetic paragenetic influences”, “targeted therapies”, “veterinary medicine”, “genetic variation”, “free radicals oxidative stress”. Inclusion criteria included focusing species-specific therapeutic responses, genetic influences, stress. To ensure that only most recent relevant evidence was included, peer-reviewed publications from last two decades considered. Each study selected for analysis critically appraised, with a particular emphasis methodological quality, experimental design, scientific contribution to understanding how biological influence outcomes. A special placed used comparative, cross-species approach assess variability responses potential adverse effects. The synthesises role epigenetic highlights importance understand By highlighting variation, stress, individual species differences, argues personalised approaches. emphasises an would improve care inform future research aimed at optimising therapies, ultimately leading better animal health efficacy. key is its need more protocols take into account profiles factors; it also calls greater integration studies.

Language: Английский

Morphofunctional changes in the immune system in colitis-associated colorectal cancer in tolerant and susceptible to hypoxia mice

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19024 - e19024

Published: Feb. 25, 2025

Background One of the effective strategies for treatment tumor diseases, including colitis-associated colorectal cancer (CAC), is immunotherapy. During inflammation, NF-κB activated, which connected with hypoxia-inducible factor—HIF, regulating immune cells functioning and influences CAC development. Organisms differ according to their hypoxia resistance HIF expression. Therefore, aim study was characterize thymus, spleen mesenteric lymph nodes morphofunctional features, as well changes in subpopulation composition peripheral blood tolerant susceptible C57Bl/6 mice CAC. Methods Hypoxia tolerance assessed by gasping time measurement hypobaric decompression chamber. Based on outcome, were assigned three groups characterized ‘tolerant hypoxia’, ‘normal’, ‘susceptible hypoxia’. A month after determining modeled intraperitoneal azoxymethane (AOM) administration cycles dextran sulfate sodium consumption. Mice sacrificed 141st day AOM administration, a morphological, morphometric immunohistochemical tumors, morphological thymus spleen, assessment carried out. Results Tumors represented glandular intraepithelial neoplasia adenocarcinomas, area larger mice. Immunohistochemical revealed more pronounced Ki-67+ staining tumors In CAC, only mice, expansion thymic cortex observed relative control group, while ones, no detected. Only germinal centers lymphoid follicles enlargement observed. during comparison absolute number B-lymphocytes relative—cytotoxic T-lymphocytes increased. The cytotoxic NK higher compared ones. revealed—only them cells, increased, macrophages decreased. Conclusions Morphofunctional differences reactions indicated response development should be taken into account experimental studies.

Language: Английский